دورية أكاديمية
Protocol to quantify immune cell distribution from the vasculature to the glioma microenvironment on sequential immunofluorescence multiplex images.
| العنوان: | Protocol to quantify immune cell distribution from the vasculature to the glioma microenvironment on sequential immunofluorescence multiplex images. |
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| المؤلفون: | Najem H; Department of Neurological Surgery, Northwestern University, Chicago, IL 60611, USA; Malnati Brain Tumor Institute of the Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA., Pacheco S; Department of Neurological Surgery, Northwestern University, Chicago, IL 60611, USA; Malnati Brain Tumor Institute of the Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA., Kowal J; Lunaphore Technologies SA, 1131 Tolochenaz, Switzerland., Winkowski D; Visiopharm A/S, 2970 Horsholm, Denmark., Burks JK; MD Anderson Cancer Center, Houston, TX 77030, USA., Heimberger AB; Department of Neurological Surgery, Northwestern University, Chicago, IL 60611, USA; Malnati Brain Tumor Institute of the Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. Electronic address: amy.heimberger@northwestern.edu. |
| المصدر: | STAR protocols [STAR Protoc] 2024 Jun 21; Vol. 5 (2), pp. 103079. Date of Electronic Publication: 2024 May 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Tumor Microenvironment*/immunology , Glioma*/immunology , Glioma*/pathology , Glioma*/blood supply , Fluorescent Antibody Technique*/methods, Humans ; Animals ; Brain Neoplasms/immunology ; Brain Neoplasms/pathology ; Brain Neoplasms/blood supply ; Image Processing, Computer-Assisted/methods ; Mice |
| مستخلص: | Although myeloid-derived immune cells can be dispersed throughout the tumor microenvironment (TME), anti-tumor effector cells are confined to the perivascular space. Here, we present a protocol to quantify immune cell distribution from tumor vasculature to its glioma microenvironment on sequential immunofluorescence multiplex images. We describe steps for sequential immunofluorescence multiplex staining, image generation, and storage. We then detail the procedures for tissue, vessel, and nuclei segmentation; cell phenotyping; data extraction; and training using RStudio and Spyder. Competing Interests: Declaration of interests A.B.H. serves on the advisory board of Caris Life Sciences. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| معلومات مُعتمدة: | P30 CA016672 United States CA NCI NIH HHS; R50 CA243707 United States CA NCI NIH HHS |
| فهرسة مساهمة: | Keywords: Cancer; Immunology; Neuroscience |
| تواريخ الأحداث: | Date Created: 20240525 Date Completed: 20240625 Latest Revision: 20240702 |
| رمز التحديث: | 20240702 |
| مُعرف محوري في PubMed: | PMC11144811 |
| DOI: | 10.1016/j.xpro.2024.103079 |
| PMID: | 38795354 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2024.103079 |