دورية أكاديمية
أعرض في EDS

Expression pattern of Runt-related transcription factor (RUNX) family members and the role of RUNX1 during kidney development.

التفاصيل البيبلوغرافية
العنوان: Expression pattern of Runt-related transcription factor (RUNX) family members and the role of RUNX1 during kidney development.
المؤلفون: Yano-Sakamoto K; Department of Nephrology, Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan. Electronic address: keikoyano@kuhp.kyoto-u.ac.jp., Kitai Y; Department of Nephrology, Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan. Electronic address: ykitai@kuhp.kyoto-u.ac.jp., Toriu N; Department of Nephrology, Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto, 606-8501, Japan. Electronic address: ntoriu@kuhp.kyoto-u.ac.jp., Yamamoto S; Department of Nephrology, Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan. Electronic address: syamamon@kuhp.kyoto-u.ac.jp., Mizuta K; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto, 606-8501, Japan; Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto, 606-8501, Japan. Electronic address: mizuta@anat2.med.kyoto-u.ac.jp., Saitou M; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto, 606-8501, Japan; Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto, 606-8501, Japan; Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, 606-8397, Japan. Electronic address: saitou@anat2.med.kyoto-u.ac.jp., Tsukiyama T; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto, 606-8501, Japan; Research Center for Animal Life Science, Shiga University of Medical Science, Shiga, 520-2192, Japan. Electronic address: ttsuki@belle.shiga-med.ac.jp., Taniuchi I; Laboratory for Transcriptional Regulation, RIKEN Center for Integrative Medical Sciences, Yokohama, 230-0045, Japan. Electronic address: ichiro.taniuchi@riken.jp., Osato M; International Research Center for Medical Sciences, Kumamoto University, Kumamoto, 860-0811, Japan. Electronic address: motokuma@kumamoto-u.ac.jp., Yanagita M; Department of Nephrology, Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto, 606-8501, Japan. Electronic address: motoy@kuhp.kyoto-u.ac.jp.
المصدر: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Aug 30; Vol. 722, pp. 150155. Date of Electronic Publication: 2024 May 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
أسماء مطبوعة: Publication: <2002- >: San Diego, CA : Elsevier
Original Publication: New York, Academic Press.
مواضيع طبية MeSH: Core Binding Factor Alpha 2 Subunit*/metabolism , Core Binding Factor Alpha 2 Subunit*/genetics , Kidney*/metabolism , Kidney*/embryology , Kidney*/growth & development, Animals ; Mice ; Macaca fascicularis ; Gene Expression Regulation, Developmental ; Core Binding Factor Alpha 1 Subunit/metabolism ; Core Binding Factor Alpha 1 Subunit/genetics ; Core Binding Factor Alpha 3 Subunit/metabolism ; Core Binding Factor Alpha 3 Subunit/genetics ; Core Binding Factor alpha Subunits/metabolism ; Core Binding Factor alpha Subunits/genetics ; Mice, Inbred C57BL ; Mice, Knockout
مستخلص: Runt-related transcription factor (RUNX) family members play critical roles in the development of multiple organs. Mammalian RUNX family members, consisting of RUNX1, RUNX2, and RUNX3, have distinct tissue-specific expression and function. In this study, we examined the spatiotemporal expression patterns of RUNX family members in developing kidneys and analyzed the role of RUNX1 during kidney development. In the developing mouse kidney, RUNX1 protein was strongly expressed in the ureteric bud (UB) tip and weakly expressed in the distal segment of the renal vesicle (RV), comma-shaped body (CSB), and S-shaped body (SSB). In contrast, RUNX2 protein was restricted to the stroma, and RUNX3 protein was only expressed in immune cells. We also analyzed the expression of RUNX family members in the cynomolgus monkey kidney. We found that expression patterns of RUNX2 and RUNX3 were conserved between rodents and primates, whereas RUNX1 was only expressed in the UB tip, not in the RV, CSB, or SSB of cynomolgus monkeys, suggesting a species differences. We further evaluated the roles of RUNX1 using two different conditional knockout mice: Runx1 f/f :HoxB7-Cre and Runx1 f/f :R26-CreER T2 and found no abnormalities in the kidney. Our findings showed that RUNX1, which is mainly expressed in the UB tip, is not essential for kidney development.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Motoko Yanagita reports financial support was provided by Japan Agency for Medical Research and Development. Motoko Yanagita reports a relationship with Mitsubishi Tanabe Pharma Corporation, Boehringer Ingelheim that includes: funding grants. None has patent pending to none. none If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Kidney development; Runt-related transcription factor; Ureteric bud
المشرفين على المادة: 0 (Core Binding Factor Alpha 2 Subunit)
0 (Runx1 protein, mouse)
0 (Core Binding Factor Alpha 1 Subunit)
0 (Core Binding Factor Alpha 3 Subunit)
0 (Core Binding Factor alpha Subunits)
0 (Runx3 protein, mouse)
0 (Runx2 protein, mouse)
تواريخ الأحداث: Date Created: 20240525 Date Completed: 20240614 Latest Revision: 20240621
رمز التحديث: 20240622
DOI: 10.1016/j.bbrc.2024.150155
PMID: 38795454
قاعدة البيانات: MEDLINE
الوصف
تدمد:1090-2104
DOI:10.1016/j.bbrc.2024.150155