دورية أكاديمية
Mechanisms of ingested polystyrene micro-nanoplastics (MNPs) uptake and translocation in an in vitro tri-culture small intestinal epithelium.
| العنوان: | Mechanisms of ingested polystyrene micro-nanoplastics (MNPs) uptake and translocation in an in vitro tri-culture small intestinal epithelium. |
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| المؤلفون: | DeLoid GM; Nanoscience and Advanced Materials Center, Environmental and Occupational Health Sciences Institute (EOHSI), Rutgers University, Piscataway, NJ 08854, USA. Electronic address: gd424@eohsi.rutgers.edu., Yang Z; Nanoscience and Advanced Materials Center, Environmental and Occupational Health Sciences Institute (EOHSI), Rutgers University, Piscataway, NJ 08854, USA; Ernest Mario School of Pharmacy, Rutgers University, Piscataway, NJ 08854, USA., Bazina L; Nanoscience and Advanced Materials Center, Environmental and Occupational Health Sciences Institute (EOHSI), Rutgers University, Piscataway, NJ 08854, USA; School of Public Health, Rutgers University, Piscataway, NJ 08854, USA., Kharaghani D; Nanoscience and Advanced Materials Center, Environmental and Occupational Health Sciences Institute (EOHSI), Rutgers University, Piscataway, NJ 08854, USA., Sadrieh F; Nanoscience and Advanced Materials Center, Environmental and Occupational Health Sciences Institute (EOHSI), Rutgers University, Piscataway, NJ 08854, USA., Demokritou P; Nanoscience and Advanced Materials Center, Environmental and Occupational Health Sciences Institute (EOHSI), Rutgers University, Piscataway, NJ 08854, USA; School of Public Health, Rutgers University, Piscataway, NJ 08854, USA. Electronic address: philip.demokritou@rutgers.edu. |
| المصدر: | Journal of hazardous materials [J Hazard Mater] 2024 Jul 15; Vol. 473, pp. 134706. Date of Electronic Publication: 2024 May 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9422688 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3336 (Electronic) Linking ISSN: 03043894 NLM ISO Abbreviation: J Hazard Mater Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier, |
| مواضيع طبية MeSH: | Polystyrenes*/chemistry , Polystyrenes*/metabolism , Intestinal Mucosa*/metabolism , Intestine, Small*/metabolism , Intestine, Small*/drug effects , Endocytosis*, Microplastics/metabolism ; Humans ; Nanoparticles/chemistry ; Animals |
| مستخلص: | Micro and nanoplastics (MNPs) are now ubiquitous contaminants of food and water. Many cellular and animal studies have shown that ingested MNPs can breach the intestinal barrier to reach the circulation. To date however, the cellular mechanisms involved in intestinal absorption of MNPs have not been investigated with physiologically relevant models, and thus remain unknown. We employed in vitro simulated digestion, a tri-culture small intestinal epithelium model, and a panel of inhibitors to assess the contributions of the possible mechanisms to absorption of 26 nm carboxylated polystyrene (PS26C) MNPs. Inhibition of ATP synthesis reduced translocation by only 35 %, suggesting uptake by both active endocytic pathways and passive diffusion. Translocation was also decreased by inhibition of dynamin and clathrin, suggesting involvement of clathrin mediated endocytosis (CME) and fast endophilin-mediated endocytosis (FEME). Inhibition of actin polymerization also significantly reduced translocation, suggesting involvement of macropinocytosis or phagocytosis. However, inhibition of the Na + -H + exchanger had no effect on translocation, thus ruling out macropinocytosis. Together these results suggest uptake by passive diffusion as well as by active phagocytosis, CME, and FEME pathways. Further studies are needed to assess uptake mechanisms for other environmentally relevant MNPs as a function of polymer, surface chemistry, and size. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Conflict of Interest The authors declare no conflict of Interest. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Absorption; Ingestion; Mechanism; Micro-nanoplastics; Microplastics |
| المشرفين على المادة: | 0 (Polystyrenes) 0 (Microplastics) |
| تواريخ الأحداث: | Date Created: 20240525 Date Completed: 20240611 Latest Revision: 20240611 |
| رمز التحديث: | 20240611 |
| DOI: | 10.1016/j.jhazmat.2024.134706 |
| PMID: | 38795489 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3336 |
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| DOI: | 10.1016/j.jhazmat.2024.134706 |