دورية أكاديمية
Inhibition of BMP-mediated SMAD pathway supports the pluripotency of pig embryonic stem cells in the absence of feeder cells.
| العنوان: | Inhibition of BMP-mediated SMAD pathway supports the pluripotency of pig embryonic stem cells in the absence of feeder cells. |
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| المؤلفون: | Choi KH; Research and Development Center, Space F Corporation, Hwaseong, Gyeonggi-do, 18471, Republic of Korea; Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, 08826, Republic of Korea. Electronic address: ckh1122@spacef.biz., Lee DK; Research and Development Center, Space F Corporation, Hwaseong, Gyeonggi-do, 18471, Republic of Korea; Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, 08826, Republic of Korea., Jeong J; Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, 08826, Republic of Korea., Ahn Y; Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, 08826, Republic of Korea., Go DM; Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, 08826, Republic of Korea., Kim DY; Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, 08826, Republic of Korea., Lee CK; Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, 08826, Republic of Korea; Institute of Green Bio Science and Technology, Seoul National University, Pyeong Chang, 25354, Republic of Korea. Electronic address: leeck@snu.ac.kr. |
| المصدر: | Theriogenology [Theriogenology] 2024 Sep 01; Vol. 225, pp. 67-80. Date of Electronic Publication: 2024 May 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: [New York, N.Y.?] : Elsevier Original Publication: Los Altos, Calif., Geron-X. |
| مواضيع طبية MeSH: | Embryonic Stem Cells*/drug effects , Signal Transduction*/drug effects , Pyrazoles*/pharmacology, Animals ; Swine ; Pyrimidines/pharmacology ; Bone Morphogenetic Proteins/metabolism ; Pluripotent Stem Cells/drug effects ; Cell Differentiation/drug effects ; Smad Proteins/metabolism ; Smad Proteins/genetics ; Feeder Cells ; Cell Culture Techniques |
| مستخلص: | Here, we examined the effects of the BMP signaling pathway inhibitor LDN-193189 on the pluripotency of porcine embryonic stem cells (ESCs) in the absence of feeder cells using molecular and transcriptomic techniques. Additionally, the effects of some extracellular matrix components on porcine ESC pluripotency were evaluated to develop an optimized and sustainable feeder-free culture system for porcine ESCs. Feeder cells were found to play an important role in supporting the pluripotency of porcine ESCs by blocking trophoblast and mesodermal differentiation through the inhibition of the BMP pathway. Additionally, treatment with LDN-193189, an inhibitor of the BMP pathway, maintained the pluripotency and homogeneity of porcine ESCs for an extended period in the absence of feeder cells by stimulating the secretion of chemokines and suppressing differentiation, based on transcriptome analysis. Conclusively, these results suggest that LDN-193189 could be a suitable replacement for feeder cells in the maintenance of porcine ESC pluripotency during culture. Additionally, these findings contribute to the understanding of pluripotency gene networks and comparative embryogenesis. Competing Interests: Declaration of competing interest Authors declare that they have no competing interests. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: BMP signaling pathway; Chemokines; Feeder-free culture; LDN-193189; Pig embryonic stem cells |
| المشرفين على المادة: | 0 (LDN 193189) 0 (Pyrazoles) 0 (Pyrimidines) 0 (Bone Morphogenetic Proteins) 0 (Smad Proteins) |
| تواريخ الأحداث: | Date Created: 20240525 Date Completed: 20240606 Latest Revision: 20240606 |
| رمز التحديث: | 20240607 |
| DOI: | 10.1016/j.theriogenology.2024.05.027 |
| PMID: | 38795512 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-3231 |
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| DOI: | 10.1016/j.theriogenology.2024.05.027 |