دورية أكاديمية
Validation of one-step reverse transcription digital PCR assays for Norovirus GI.
| العنوان: | Validation of one-step reverse transcription digital PCR assays for Norovirus GI. |
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| المؤلفون: | Ko B; Bio-Metrology Group, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea; Korea University Graduate School of Life Sciences and Biotechnology, Seoul, Republic of Korea., Shin T; Bio-Metrology Group, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea., Kim B; Bio-Metrology Group, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea., Lee DH; Bio-Metrology Group, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea. Electronic address: dahye.lee04@kriss.re.kr. |
| المصدر: | Analytical biochemistry [Anal Biochem] 2024 Sep; Vol. 692, pp. 115576. Date of Electronic Publication: 2024 May 23. |
| نوع المنشور: | Journal Article; Validation Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0370535 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0309 (Electronic) Linking ISSN: 00032697 NLM ISO Abbreviation: Anal Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : San Diego, CA : Elsevier Original Publication: Orlando Fl : Academic Press |
| مواضيع طبية MeSH: | Norovirus*/genetics , Norovirus*/isolation & purification , Reverse Transcriptase Polymerase Chain Reaction*/methods, RNA, Viral/genetics ; RNA, Viral/analysis ; Humans ; Caliciviridae Infections/diagnosis ; Caliciviridae Infections/virology |
| مستخلص: | Regular monitoring of Norovirus presence in environmental and food samples is crucial due to its high transmission rates and outbreak potential. For detecting Norovirus GI, reverse transcription qPCR method is commonly used, but its sensitivity can be affected by assay performance. This study shows significantly reduced assay performance in digital PCR or qPCR when using primers targeting Norovirus GI genome 5291-5319 (NC_001959), located on the hairpin of the predicted RNA structure. It is highly recommended to avoid this region in commercial kit development or diagnosis to minimizing potential risk of false negatives. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Droplet based digital PCR; Nanoplate based digital PCR; Norovirus GI assay; One-step reverse transcription digital PCR; Reference material; Secondary structure |
| المشرفين على المادة: | 0 (RNA, Viral) |
| تواريخ الأحداث: | Date Created: 20240525 Date Completed: 20240614 Latest Revision: 20240614 |
| رمز التحديث: | 20240615 |
| DOI: | 10.1016/j.ab.2024.115576 |
| PMID: | 38796118 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-0309 |
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| DOI: | 10.1016/j.ab.2024.115576 |