دورية أكاديمية
أعرض في EDS

Metabolism of L-arabinose converges with virulence regulation to promote enteric pathogen fitness.

التفاصيل البيبلوغرافية
العنوان: Metabolism of L-arabinose converges with virulence regulation to promote enteric pathogen fitness.
المؤلفون: Cottam C; Newcastle University Biosciences Institute, Newcastle University, NE2 4HH, Newcastle-upon-Tyne, UK., White RT; Institute of Environmental Science and Research, Wellington, New Zealand.; Australian Infectious Diseases Research Centre and School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, Australia., Beck LC; Newcastle University Translation and Clinical Research Institute, Newcastle University, NE2 4HH, Newcastle-upon-Tyne, UK., Stewart CJ; Newcastle University Translation and Clinical Research Institute, Newcastle University, NE2 4HH, Newcastle-upon-Tyne, UK., Beatson SA; Australian Infectious Diseases Research Centre and School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, Australia., Lowe EC; Newcastle University Biosciences Institute, Newcastle University, NE2 4HH, Newcastle-upon-Tyne, UK., Grinter R; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia., Connolly JPR; Newcastle University Biosciences Institute, Newcastle University, NE2 4HH, Newcastle-upon-Tyne, UK. James.Connolly2@newcastle.ac.uk.
المصدر: Nature communications [Nat Commun] 2024 May 25; Vol. 15 (1), pp. 4462. Date of Electronic Publication: 2024 May 25.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Arabinose*/metabolism , Citrobacter rodentium*/pathogenicity , Citrobacter rodentium*/metabolism , Citrobacter rodentium*/genetics , Enterohemorrhagic Escherichia coli*/pathogenicity , Enterohemorrhagic Escherichia coli*/metabolism , Enterohemorrhagic Escherichia coli*/genetics, Animals ; Mice ; Humans ; Virulence ; Gene Expression Regulation, Bacterial ; Virulence Factors/metabolism ; Virulence Factors/genetics ; Enterobacteriaceae Infections/microbiology ; Escherichia coli Proteins/metabolism ; Escherichia coli Proteins/genetics ; Type III Secretion Systems/metabolism ; Type III Secretion Systems/genetics ; Escherichia coli Infections/microbiology ; Female
مستخلص: Virulence and metabolism are often interlinked to control the expression of essential colonisation factors in response to host-associated signals. Here, we identified an uncharacterised transporter of the dietary monosaccharide ʟ-arabinose that is widely encoded by the zoonotic pathogen enterohaemorrhagic Escherichia coli (EHEC), required for full competitive fitness in the mouse gut and highly expressed during human infection. Discovery of this transporter suggested that EHEC strains have an enhanced ability to scavenge ʟ-arabinose and therefore prompted us to investigate the impact of this nutrient on pathogenesis. Accordingly, we discovered that ʟ-arabinose enhances expression of the EHEC type 3 secretion system, increasing its ability to colonise host cells, and that the underlying mechanism is dependent on products of its catabolism rather than the sensing of ʟ-arabinose as a signal. Furthermore, using the murine pathogen Citrobacter rodentium, we show that ʟ-arabinose metabolism provides a fitness benefit during infection via virulence factor regulation, as opposed to supporting pathogen growth. Finally, we show that this mechanism is not restricted to ʟ-arabinose and extends to other pentose sugars with a similar metabolic fate. This work highlights the importance integrating central metabolism with virulence regulation in order to maximise competitive fitness of enteric pathogens within the host-niche.
(© 2024. The Author(s).)
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معلومات مُعتمدة: SBF005\1029 United Kingdom AMS_ Academy of Medical Sciences; RGS\R2\202100 Royal Society; MR/X007197/1 RCUK | Medical Research Council (MRC); 226644/Z/22/Z Wellcome Trust (Wellcome)
المشرفين على المادة: B40ROO395Z (Arabinose)
0 (Virulence Factors)
0 (Escherichia coli Proteins)
0 (Type III Secretion Systems)
تواريخ الأحداث: Date Created: 20240525 Date Completed: 20240525 Latest Revision: 20240528
رمز التحديث: 20240528
مُعرف محوري في PubMed: PMC11127945
DOI: 10.1038/s41467-024-48933-7
PMID: 38796512
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-024-48933-7