دورية أكاديمية
Single-Domain Antibody-Gold Nanoparticle Bioconjugates as Immunosensors for the Detection of Hantaviruses.
| العنوان: | Single-Domain Antibody-Gold Nanoparticle Bioconjugates as Immunosensors for the Detection of Hantaviruses. |
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| المؤلفون: | Bastos-Soares EA; Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia, Porto Velho, RO, Brazil.; Programa de Pós-graduação em Biologia Experimental, PGBIOEXP, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil., da Silva Morais MS; Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia, Porto Velho, RO, Brazil.; Programa de Pós-graduação em Biologia Experimental, PGBIOEXP, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil., Funes-Huacca M; Departamento de Química, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil., Sousa RMO; Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia, Porto Velho, RO, Brazil., Brilhante-Da-Silva N; Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia, Porto Velho, RO, Brazil., Roberto SA; Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia, Porto Velho, RO, Brazil.; Programa de Pós-graduação em Biologia Experimental, PGBIOEXP, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil., Prado NDR; Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia, Porto Velho, RO, Brazil., Dos Santos CND; Instituto Carlos Chagas, FIOCRUZ, Fiocruz Paraná, Curitiba, PR, Brazil., Marinho ACM; Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Ceará, Eusébio, CE, Brazil., Soares AM; Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia, Porto Velho, RO, Brazil.; Centro Universitário São Lucas, UniSL, Porto Velho, RO, Brazil.; Instituto Nacional de Ciência e Tecnologia em Epidemiologia da Amazônia Ocidental, INCT-EpiAmO, Porto Velho, RO, Brazil.; Programa de Pós-graduação em Biologia Experimental, PGBIOEXP, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil., Stabeli RG; Fundação Oswaldo Cruz, FIOCRUZ, Plataforma Bi-institucional de Medicina Translacional, Ribeirão Preto, SP, Brazil., Pereira SDS; Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia, Porto Velho, RO, Brazil.; Programa de Pós-graduação em Biologia Experimental, PGBIOEXP, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil., Fernandes CFC; Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Ceará, Eusébio, CE, Brazil. carlaceledonio@fiocruz.br. |
| المصدر: | Molecular diagnosis & therapy [Mol Diagn Ther] 2024 Jul; Vol. 28 (4), pp. 479-494. Date of Electronic Publication: 2024 May 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Adis, Springer International Country of Publication: New Zealand NLM ID: 101264260 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1179-2000 (Electronic) Linking ISSN: 11771062 NLM ISO Abbreviation: Mol Diagn Ther Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Auckland : Adis, Springer International Original Publication: Auckland, N.Z. : Adis International, c2006- |
| مواضيع طبية MeSH: | Gold*/chemistry , Metal Nanoparticles*/chemistry , Single-Domain Antibodies*/immunology , Single-Domain Antibodies*/chemistry , Orthohantavirus*/immunology , Biosensing Techniques*/methods, Humans ; Antibodies, Viral/immunology ; Animals ; Hantavirus Infections/diagnosis |
| مستخلص: | Introduction: Hantavirus, a zoonotic pathogen, causes severe syndromes like hemorrhagic fever with renal syndrome (HFRS), sometimes fatal in humans. Considering the importance of detecting the hantavirus antigen, the construction of an immunosensor is essential. The structural and functional characteristics of camelid nanobodies (VHHs) encourage their application in the areas of nanobiotechnology, therapeutics, diagnostics, and basic research. Therefore, this study aimed to standardize stable bioconjugates using gold nanoparticles (AuNPs) and VHHs, in order to develop immunobiosensors for the diagnosis of hantavirus infection. Methods: Immobilized metal affinity chromatography (IMAC) was performed to obtain purified recombinant anti-hantavirus nucleocapsid nanobodies (anti-prNΔ Results: The bioconjugation stability parameters (VHH-AuNPs), analyzed by spectrophotometry, showed that the ideal pH value and VHH concentration were obtained at 7.4 and 50 μg/mL, respectively, after addition of 1 M NaCl, which induces AuNP aggregation. TEM performed before and after bioconjugation showed uniform, homogeneous, well-dispersed, and spherical AuNPs with an average diameter of ~ 14 ± 0.57 nm. Furthermore, high-resolution images revealed a thin white halo on the surface of the AuNPs, indicating the coating of the AuNPs with protein. A biosensor simulation test (dot blot-like [DB-like]) was performed in stationary phase to verify the binding and detection limits of the recombinant nucleocapsid protein from the Araucária hantavirus strain (prN∆ Discussion: Using AuNPs/VHH bioconjugates, a specific interaction was detected between 5 and 10 min of reaction in a dose-dependent manner. It was observed that this test was sensitive enough to detect prNΔ (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.) |
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| المشرفين على المادة: | 7440-57-5 (Gold) 0 (Single-Domain Antibodies) 0 (Antibodies, Viral) |
| تواريخ الأحداث: | Date Created: 20240525 Date Completed: 20240627 Latest Revision: 20240627 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1007/s40291-024-00713-1 |
| PMID: | 38796660 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1179-2000 |
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| DOI: | 10.1007/s40291-024-00713-1 |