دورية أكاديمية

A Large Proportion of the Neonatal Iron Pool Is Acquired from the Gestational Diet in a Murine Model.

التفاصيل البيبلوغرافية
العنوان: A Large Proportion of the Neonatal Iron Pool Is Acquired from the Gestational Diet in a Murine Model.
المؤلفون: Lueschow-Guijosa SR; Stead Family Department of Pediatrics, University of Iowa, Iowa City, IA, United States; Iowa Inflammation Program, University of Iowa, Iowa City, IA, United States., Michels KR; UmojaBiopharma, Seattle, WA, United States., Latta DE; Department of Civil and Environmental Engineering, University of Iowa, Iowa City, IA, United States., Bermick JR; Stead Family Department of Pediatrics, University of Iowa, Iowa City, IA, United States; Iowa Inflammation Program, University of Iowa, Iowa City, IA, United States. Electronic address: jennifer-bermick@uiowa.edu.
المصدر: The Journal of nutrition [J Nutr] 2024 Jul; Vol. 154 (7), pp. 2065-2075. Date of Electronic Publication: 2024 May 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0404243 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1541-6100 (Electronic) Linking ISSN: 00223166 NLM ISO Abbreviation: J Nutr Subsets: MEDLINE
أسماء مطبوعة: Publication: 2023- : [New York, NY] : Elsevier
Original Publication: 1928-1933 : Springfield, Ill. : C. C. Thomas
مواضيع طبية MeSH: Animals, Newborn* , Iron*/metabolism , Iron*/blood , Diet*, Animals ; Female ; Pregnancy ; Mice ; Liver/metabolism ; Mice, Inbred C57BL ; Maternal Nutritional Physiological Phenomena ; Iron, Dietary/administration & dosage ; Male ; Iron Isotopes
مستخلص: Background: Iron is crucial for growth and development, but excess iron is harmful. Neonatal mice have elevated concentrations of circulating iron, but the source of this iron is unclear. This lack of understanding makes it difficult to optimize early life iron balance.
Objectives: Identify the origins of neonatal tissue-specific iron pools using dietary manipulation and cross-fostering murine models.
Methods: To determine whether tissue-specific neonatal iron was primarily acquired during gestation or after birth, pups born to iron-sufficient or iron-deficient dams were cross-fostered, and tissues were harvested at postnatal days 3-5 to measure iron content. A separate set of female mice were fed a diet enriched with the stable iron isotope 57 ( 57 Fe) for 4 generations to replace naturally abundant liver iron isotope 56 ( 56 Fe) stores with 57 Fe. To quantify the proportions of neonatal iron acquired during gestation, pups born to dams with 56 Fe or 57 Fe stores were cross-fostered, and tissues were harvested at postnatal day 3-5 to determine 56 Fe: 57 Fe ratios by inductively coupled plasma mass spectrometry. Finally, to quantify the proportion of neonatal iron acquired from the maternal diet, female mice with 56 Fe or 57 Fe stores switched diets upon mating, and pup tissues were harvested on P0 to determine 56 Fe: 57 Fe ratios by inductively coupled plasma mass spectrometry.
Results: Perinatal iron deficiency resulted in smaller pups, and gestational iron deficiency resulted in lower neonatal serum and liver iron. Cross-fostering between dams with 56 Fe and 57 Fe stores demonstrated that ≤70% of neonatal serum, liver, and brain iron were acquired during gestation. Dietary manipulation experiments using dams with 56 Fe and 57 Fe stores showed that over half of neonatal serum, liver, and brain iron were from the dam's gestational diet rather than preconception iron stores.
Conclusions: This study provides quantitative values for the sources of neonatal iron, which may inform approaches to optimize neonatal iron status.
(Copyright © 2024 American Society for Nutrition. Published by Elsevier Inc. All rights reserved.)
معلومات مُعتمدة: R01 AI150687 United States AI NIAID NIH HHS
فهرسة مساهمة: Keywords: dietary iron; iron isotopes; murine model; newborn; pregnancy
المشرفين على المادة: E1UOL152H7 (Iron)
0 (Iron, Dietary)
0 (Iron Isotopes)
تواريخ الأحداث: Date Created: 20240526 Date Completed: 20240705 Latest Revision: 20240705
رمز التحديث: 20240706
DOI: 10.1016/j.tjnut.2024.05.021
PMID: 38797484
قاعدة البيانات: MEDLINE
الوصف
تدمد:1541-6100
DOI:10.1016/j.tjnut.2024.05.021