دورية أكاديمية
أعرض في EDS

Organic matter degradation in the deep, sulfidic waters of the Black Sea: insights into the ecophysiology of novel anaerobic bacteria.

التفاصيل البيبلوغرافية
العنوان: Organic matter degradation in the deep, sulfidic waters of the Black Sea: insights into the ecophysiology of novel anaerobic bacteria.
المؤلفون: Yadav S; Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, 1797AB Den Burg, P.O. Box 59, Texel, The Netherlands.; Department of Microbiology, Radboud Institute for Biological and Environmental Sciences, Radboud University, Nijmegen, The Netherlands., Koenen M; Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, 1797AB Den Burg, P.O. Box 59, Texel, The Netherlands., Bale NJ; Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, 1797AB Den Burg, P.O. Box 59, Texel, The Netherlands., Reitsma W; Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, 1797AB Den Burg, P.O. Box 59, Texel, The Netherlands., Engelmann JC; Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, 1797AB Den Burg, P.O. Box 59, Texel, The Netherlands., Stefanova K; Institute of Oceanology 'Fridtjof Nansen', Bulgarian Academy of Sciences, Varna, Bulgaria., Damsté JSS; Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, 1797AB Den Burg, P.O. Box 59, Texel, The Netherlands.; Faculty of Geosciences, Department of Earth Sciences, Utrecht University, P.O. Box 80.021, 3508 TA, Utrecht, The Netherlands., Villanueva L; Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, 1797AB Den Burg, P.O. Box 59, Texel, The Netherlands. laura.villanueva@nioz.nl.; Faculty of Geosciences, Department of Earth Sciences, Utrecht University, P.O. Box 80.021, 3508 TA, Utrecht, The Netherlands. laura.villanueva@nioz.nl.
المصدر: Microbiome [Microbiome] 2024 May 27; Vol. 12 (1), pp. 98. Date of Electronic Publication: 2024 May 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101615147 Publication Model: Electronic Cited Medium: Internet ISSN: 2049-2618 (Electronic) Linking ISSN: 20492618 NLM ISO Abbreviation: Microbiome Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London: BioMed Central, 2013-
مواضيع طبية MeSH: Seawater*/microbiology , Bacteria, Anaerobic*/metabolism , Bacteria, Anaerobic*/classification , Bacteria, Anaerobic*/genetics, Black Sea ; Phylogeny ; Biodegradation, Environmental ; RNA, Ribosomal, 16S/genetics ; DNA, Bacterial/genetics ; Organic Chemicals/metabolism
مستخلص: Background: Recent studies have reported the identity and functions of key anaerobes involved in the degradation of organic matter (OM) in deep (> 1000 m) sulfidic marine habitats. However, due to the lack of available isolates, detailed investigation of their physiology has been precluded. In this study, we cultivated and characterized the ecophysiology of a wide range of novel anaerobes potentially involved in OM degradation in deep (2000 m depth) sulfidic waters of the Black Sea.
Results: We have successfully cultivated a diverse group of novel anaerobes belonging to various phyla, including Fusobacteriota (strain S5), Bacillota (strains A1 T and A2), Spirochaetota (strains M1 T , M2, and S2), Bacteroidota (strains B1 T , B2, S6, L6, SYP, and M2P), Cloacimonadota (Cloa-SY6), Planctomycetota (Plnct-SY6), Mycoplasmatota (Izemo-BS), Chloroflexota (Chflx-SY6), and Desulfobacterota (strains S3 T and S3-i). These microorganisms were able to grow at an elevated hydrostatic pressure of up to 50 MPa. Moreover, this study revealed that different anaerobes were specialized in degrading specific types of OM. Strains affiliated with the phyla Fusobacteriota, Bacillota, Planctomycetota, and Mycoplasmatota were found to be specialized in the degradation of cellulose, cellobiose, chitin, and DNA, respectively, while strains affiliated with Spirochaetota, Bacteroidota, Cloacimonadota, and Chloroflexota preferred to ferment less complex forms of OM. We also identified members of the phylum Desulfobacterota as terminal oxidizers, potentially involved in the consumption of hydrogen produced during fermentation. These results were supported by the identification of genes in the (meta)genomes of the cultivated microbial taxa which encode proteins of specific metabolic pathways. Additionally, we analyzed the composition of membrane lipids of selected taxa, which could be critical for their survival in the harsh environment of the deep sulfidic waters and could potentially be used as biosignatures for these strains in the sulfidic waters of the Black Sea.
Conclusions: This is the first report that demonstrates the cultivation and ecophysiology of such a diverse group of microorganisms from any sulfidic marine habitat. Collectively, this study provides a step forward in our understanding of the microbes thriving in the extreme conditions of the deep sulfidic waters of the Black Sea. Video Abstract.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 024.002.002 Dutch Ministry of Education, Culture and Science (OCW); 024.002.002 Dutch Ministry of Education, Culture and Science (OCW); 024.002.002 Dutch Ministry of Education, Culture and Science (OCW); 024.002.002 Dutch Ministry of Education, Culture and Science (OCW); 024.002.002 Dutch Ministry of Education, Culture and Science (OCW); 024.002.002 Dutch Ministry of Education, Culture and Science (OCW); 024.002.002 Dutch Ministry of Education, Culture and Science (OCW); 694569 European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program; 694569 European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program
فهرسة مساهمة: Keywords: Cloacimonadota; Clostridiales; Desulfobacterota; Ignavibacteriota; Marinifilaceae; Planctomycetota; Black Sea; Organic matters; Piezotolerant; Sulfidic waters
المشرفين على المادة: 0 (RNA, Ribosomal, 16S)
0 (DNA, Bacterial)
0 (Organic Chemicals)
تواريخ الأحداث: Date Created: 20240526 Date Completed: 20240526 Latest Revision: 20240529
رمز التحديث: 20240529
مُعرف محوري في PubMed: PMC11129491
DOI: 10.1186/s40168-024-01816-x
PMID: 38797849
قاعدة البيانات: MEDLINE
الوصف
تدمد:2049-2618
DOI:10.1186/s40168-024-01816-x