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Aflatoxin B1 induces ROS-dependent mitophagy by modulating the PINK1/Parkin pathway in HepG2 cells.

التفاصيل البيبلوغرافية
العنوان: Aflatoxin B1 induces ROS-dependent mitophagy by modulating the PINK1/Parkin pathway in HepG2 cells.
المؤلفون: Wang Y; Institute of Public Health, Chengdu University of Traditional Chinese Medicine, Chengdu, China., Long L; Deyang Center for Disease Control and Prevention, Deyang, China., Luo Q; Institute of Public Health, Chengdu University of Traditional Chinese Medicine, Chengdu, China., Huang X; Institute of Public Health, Chengdu University of Traditional Chinese Medicine, Chengdu, China., Zhang Y; Institute of Public Health, Chengdu University of Traditional Chinese Medicine, Chengdu, China., Meng X; Institute of Public Health, Chengdu University of Traditional Chinese Medicine, Chengdu, China., Chen D; Institute of Public Health, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
المصدر: Basic & clinical pharmacology & toxicology [Basic Clin Pharmacol Toxicol] 2024 Aug; Vol. 135 (2), pp. 195-209. Date of Electronic Publication: 2024 May 28.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Blackwell Country of Publication: England NLM ID: 101208422 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1742-7843 (Electronic) Linking ISSN: 17427835 NLM ISO Abbreviation: Basic Clin Pharmacol Toxicol Subsets: MEDLINE
أسماء مطبوعة: Publication: <2005-> : Oxford : Blackwell
Original Publication: Copenhagen, Denmark : Oxford, UK : Nordic Pharmacological Society Distributed by Blackwell Munksgaard, 2004-
مواضيع طبية MeSH: Mitophagy*/drug effects , Reactive Oxygen Species*/metabolism , Ubiquitin-Protein Ligases*/metabolism , Ubiquitin-Protein Ligases*/genetics , Membrane Potential, Mitochondrial*/drug effects , Protein Kinases*/metabolism , Aflatoxin B1*/toxicity , Adenosine Triphosphate*/metabolism , NF-E2-Related Factor 2*/metabolism , NF-E2-Related Factor 2*/genetics, Humans ; Hep G2 Cells ; Signal Transduction/drug effects ; Phosphoprotein Phosphatases/metabolism ; Phosphoprotein Phosphatases/genetics ; Mitochondria/drug effects ; Mitochondria/metabolism ; Acetylcysteine/pharmacology ; Mitochondrial Proteins/metabolism ; Mitochondrial Proteins/genetics
مستخلص: Aflatoxin B1 (AFB1) is extremely harmful to both humans and animals. Mitophagy is a selective process of self-elimination and has an important role in controlling mitochondrial quality. The present study aimed to investigate the effect of reactive oxygen species (ROS) accumulation on AFB1-induced mitophagy in HepG2 cells to provide a new perspective from which to design novel therapeutic strategies to treat AFB1 poisoning. ROS release was induced in HepG2 cells with AFB1 (10 μmol/L). Cell autophagy activity, mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) levels, Parkin translocation and both the transcription and expression of mitophagy-related proteins were measured when N-acetyl-L-cysteine (NAC) partially decreased the ROS level, while the knockdown of nuclear factor erythroid 2-related factor 2 (Nrf2) resulted in a large accumulation of ROS. The results reveal that NAC pretreatment ameliorated the decline in both the MMP and the ATP levels while also activating phosphoglycerate mutase 5 (PGAM5)-PTEN-induced kinase 1 (PINK1)/Parkin, while the Nrf2 knockdown group exhibited the opposite trend. These results suggest that AFB1-induced mitophagy in HepG2 cells depends on ROS, and proper ROS activates mitophagy to play a protective role.
(© 2024 Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society). Published by John Wiley & Sons Ltd.)
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فهرسة مساهمة: Keywords: Nrf2; PINK1/Parkin; aflatoxins B1; mitophagy; reactive oxygen species
المشرفين على المادة: EC 2.3.2.27 (parkin protein)
0 (Reactive Oxygen Species)
EC 2.3.2.27 (Ubiquitin-Protein Ligases)
EC 2.7.11.1 (PTEN-induced putative kinase)
EC 2.7.- (Protein Kinases)
9N2N2Y55MH (Aflatoxin B1)
8L70Q75FXE (Adenosine Triphosphate)
0 (NF-E2-Related Factor 2)
0 (NFE2L2 protein, human)
EC 3.1.3.16 (PGAM5 protein, human)
EC 3.1.3.16 (Phosphoprotein Phosphatases)
WYQ7N0BPYC (Acetylcysteine)
0 (Mitochondrial Proteins)
تواريخ الأحداث: Date Created: 20240528 Date Completed: 20240715 Latest Revision: 20240715
رمز التحديث: 20240715
DOI: 10.1111/bcpt.14034
PMID: 38804152
قاعدة البيانات: MEDLINE
الوصف
تدمد:1742-7843
DOI:10.1111/bcpt.14034