دورية أكاديمية
Vairimorpha (Nosema) ceranae can promote Serratia development in honeybee gut: an underrated threat for bees?
| العنوان: | Vairimorpha (Nosema) ceranae can promote Serratia development in honeybee gut: an underrated threat for bees? |
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| المؤلفون: | Braglia C; Dipartimento di Scienze e Tecnologie Agro-Alimentari (DISTAL), University of Bologna, Bologna, Italy., Alberoni D; Dipartimento di Scienze e Tecnologie Agro-Alimentari (DISTAL), University of Bologna, Bologna, Italy., Garrido PM; Centro de Investigación en Abejas Sociales (CIAS), Faculty of Exact and Natural Sciences (FCEyN), National University of Mar del Plata (UNMdP), Mar del Plata, Buenos Aires, Argentina.; Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM), National Scientific and Technical Research Council (CONICET), UNMdP, Centro Asoc. Simple Scientific research Commission Buenos Aires Province (CIC PBA), Mar del Plata, Buenos Aires, Argentina., Porrini MP; Centro de Investigación en Abejas Sociales (CIAS), Faculty of Exact and Natural Sciences (FCEyN), National University of Mar del Plata (UNMdP), Mar del Plata, Buenos Aires, Argentina.; Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM), National Scientific and Technical Research Council (CONICET), UNMdP, Centro Asoc. Simple Scientific research Commission Buenos Aires Province (CIC PBA), Mar del Plata, Buenos Aires, Argentina., Baffoni L; Dipartimento di Scienze e Tecnologie Agro-Alimentari (DISTAL), University of Bologna, Bologna, Italy., Scott D; Advance Science Ltd., Galway, Ireland., Eguaras MJ; Centro de Investigación en Abejas Sociales (CIAS), Faculty of Exact and Natural Sciences (FCEyN), National University of Mar del Plata (UNMdP), Mar del Plata, Buenos Aires, Argentina.; Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM), National Scientific and Technical Research Council (CONICET), UNMdP, Centro Asoc. Simple Scientific research Commission Buenos Aires Province (CIC PBA), Mar del Plata, Buenos Aires, Argentina., Di Gioia D; Dipartimento di Scienze e Tecnologie Agro-Alimentari (DISTAL), University of Bologna, Bologna, Italy., Mifsud D; Institute of Earth Systems, L-Universita ta' Malta, Msida, Malta. |
| المصدر: | Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2024 May 13; Vol. 14, pp. 1323157. Date of Electronic Publication: 2024 May 13 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media SA |
| مواضيع طبية MeSH: | Serratia*/pathogenicity , Serratia*/genetics , Serratia*/growth & development , Nosema*/pathogenicity , Nosema*/growth & development , Nosema*/physiology , Nosema*/genetics, Animals ; Bees/microbiology ; Serratia marcescens/pathogenicity ; Serratia marcescens/growth & development ; Serratia marcescens/genetics ; Gastrointestinal Tract/microbiology ; Serratia Infections/microbiology ; Cyclohexanes/pharmacology ; Serratia liquefaciens/growth & development ; Serratia liquefaciens/genetics ; Fatty Acids, Unsaturated ; Sesquiterpenes |
| مستخلص: | The genus Serratia harbors opportunistic pathogenic species, among which Serratia marcescens is pathogenic for honeybees although little studied. Recently, virulent strains of S. marcescens colonizing the Varroa destructor mite's mouth were found vectored into the honeybee body, leading to septicemia and death. Serratia also occurs as an opportunistic pathogen in the honeybee's gut with a low absolute abundance. The Serratia population seems controlled by the host immune system, but its presence may represent a hidden threat, ready to arise when honeybees are weakened by biotic and abiotic stressors. To shed light on the Serratia pathogen, this research aims at studying Serratia 's development dynamics in the honeybee body and its interactions with the co-occurring fungal pathogen Vairimorpha ceranae . Firstly, the degree of pathogenicity and the ability to permeate the gut epithelial barrier of three Serratia strains, isolated from honeybees and belonging to different species ( S. marcescens , Serratia liquefaciens , and Serratia nematodiphila ), were assessed by artificial inoculation of newborn honeybees with different Serratia doses (10 4 , 10 6 , and 10 8 cells/mL). The absolute abundance of Serratia in the gut and in the hemocoel was assessed in qPCR with primers targeting the luxS gene. Moreover, the absolute abundance of Serratia was assessed in the gut of honeybees infected with V. ceranae at different development stages and supplied with beneficial microorganisms and fumagillin. Our results showed that all tested Serratia strains could pass through the gut epithelial barrier and proliferate in the hemocoel, with S. marcescens being the most pathogenic. Moreover, under cage conditions, Serratia better proliferates when a V. ceranae infection is co-occurring, with a positive and significant correlation. Finally, fumagillin and some of the tested beneficial microorganisms could control both Serratia and Vairimorpha development. Our findings suggest a correlation between the two pathogens under laboratory conditions, a co-occurring infection that should be taken into consideration by researches when testing antimicrobial compounds active against V. ceranae , and the related honeybees survival rate. Moreover, our findings suggest a positive control of Serratia by the environmental microorganism Apilactobacillus kunkeei in a in vivo model, confirming the potential of this specie as beneficial bacteria for honeybees. Competing Interests: Author DS is/was employed by the company Advance Science Forbairt Eolaiochta Teo Limited. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Braglia, Alberoni, Garrido, Porrini, Baffoni, Scott, Eguaras, Di Gioia and Mifsud.) |
| References: | Saudi J Biol Sci. 2021 Jan;28(1):523-530. (PMID: 33424335) Microb Ecol. 2023 Jul;86(1):601-616. (PMID: 35819480) PLoS One. 2016 Dec 21;11(12):e0167752. (PMID: 28002470) BMC Vet Res. 2022 Jan 21;18(1):52. (PMID: 35062935) Sci Total Environ. 2022 Nov 25;849:157941. (PMID: 35952893) Proc Biol Sci. 2020 Aug 26;287(1933):20201184. (PMID: 32842927) Microb Ecol. 2016 Aug;72(2):448-59. (PMID: 27129319) Curr Opin Insect Sci. 2018 Apr;26:97-104. (PMID: 29764668) Parasitology. 2019 Mar;146(3):380-388. (PMID: 30246672) Curr Opin Insect Sci. 2018 Apr;26:114-119. (PMID: 29764650) Appl Environ Microbiol. 2014 Mar;80(5):1679-83. (PMID: 24375136) Annu Rev Microbiol. 1978;32:221-48. (PMID: 360966) Anim Microbiome. 2022 Feb 22;4(1):16. (PMID: 35193702) Int J Food Microbiol. 2012 Jul 2;157(2):156-61. (PMID: 22608658) R Soc Open Sci. 2019 Feb 6;6(2):181803. (PMID: 30891288) PLoS Biol. 2017 Mar 14;15(3):e2001861. (PMID: 28291793) Pathogens. 2021 Sep 01;10(9):. (PMID: 34578150) Rev Sci Tech. 2008 Aug;27(2):485-97, 499-510. (PMID: 18819674) Environ Toxicol Chem. 2020 May;39(6):1244-1256. (PMID: 32170767) Environ Pollut. 2020 Dec;267:115622. (PMID: 33254673) J Insect Physiol. 2016 Mar;86:40-7. (PMID: 26776096) Vet Microbiol. 2015 May 15;177(1-2):1-6. (PMID: 25752367) Appl Environ Microbiol. 1995 Jul;61(7):2643-8. (PMID: 7618877) Sci Total Environ. 2021 Mar 25;762:144116. (PMID: 33383302) PLoS One. 2017 Nov 10;12(11):e0187505. (PMID: 29125851) Environ Microbiol Rep. 2013 Feb;5(1):17-29. (PMID: 23757127) mBio. 2017 Dec 12;8(6):. (PMID: 29233893) Vet Sci. 2020 Dec 08;7(4):. (PMID: 33302502) J Invertebr Pathol. 2010 Jan;103 Suppl 1:S96-119. (PMID: 19909970) Microbiol Spectr. 2021 Sep 3;9(1):e0017621. (PMID: 34378962) Appl Environ Microbiol. 1984 Jan;47(1):22-7. (PMID: 16346457) J Invertebr Pathol. 2013 May;113(1):35-41. (PMID: 23321524) Benef Microbes. 2016 Feb;7(1):45-51. (PMID: 26565084) Sci Total Environ. 2024 Jan 1;906:167530. (PMID: 37832690) Syst Appl Microbiol. 2019 Mar;42(2):205-216. (PMID: 30551956) Int Wound J. 2016 Oct;13(5):668-79. (PMID: 25195876) Nat Rev Microbiol. 2008 Apr;6(4):302-13. (PMID: 18327270) Benef Microbes. 2018 Feb 27;9(2):269-278. (PMID: 29380644) mBio. 2018 Oct 9;9(5):. (PMID: 30301854) Probiotics Antimicrob Proteins. 2024 Feb;16(1):259-274. (PMID: 36637793) Microb Ecol. 2020 Nov;80(4):908-919. (PMID: 32666305) Proc Natl Acad Sci U S A. 2018 Oct 9;115(41):10305-10310. (PMID: 30249635) Microorganisms. 2021 May 07;9(5):. (PMID: 34067140) R Soc Open Sci. 2017 Feb 8;4(2):170003. (PMID: 28386455) Environ Microbiol Rep. 2013 Aug;5(4):506-10. (PMID: 23864563) Trends Microbiol. 2012 Jul;20(7):336-42. (PMID: 22564248) |
| فهرسة مساهمة: | Keywords: Apilactobacillus; Nosema ceranae; Serratia; beneficial bacteria; fumagillin; nosemosis |
| المشرفين على المادة: | 7OW73204U1 (fumagillin) 0 (Cyclohexanes) 0 (Fatty Acids, Unsaturated) 0 (Sesquiterpenes) |
| SCR Organism: | Nosema ceranae |
| تواريخ الأحداث: | Date Created: 20240529 Date Completed: 20240529 Latest Revision: 20240530 |
| رمز التحديث: | 20240530 |
| مُعرف محوري في PubMed: | PMC11131372 |
| DOI: | 10.3389/fcimb.2024.1323157 |
| PMID: | 38808063 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2235-2988 |
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| DOI: | 10.3389/fcimb.2024.1323157 |