دورية أكاديمية
Recombinant Beauveria bassiana expressing Bacillus thuringiensis toxin Cyt1Aa: a promising approach for enhancing Aedes mosquito control.
| العنوان: | Recombinant Beauveria bassiana expressing Bacillus thuringiensis toxin Cyt1Aa: a promising approach for enhancing Aedes mosquito control. |
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| المؤلفون: | Deng S-Q; Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China.; Anhui Province Key Laboratory of Zoonoses, the Key Laboratory of Zoonoses of High Institutions in Anhui, Anhui Medical University, Hefei, China., Li N; Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China., Yang X-K; Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China.; Anhui Province Key Laboratory of Zoonoses, the Key Laboratory of Zoonoses of High Institutions in Anhui, Anhui Medical University, Hefei, China., Lu H-Z; Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China., Liu J-H; Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China., Peng Z-Y; Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China., Wang L-M; Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China., Zhang M; Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China.; Anhui Province Key Laboratory of Zoonoses, the Key Laboratory of Zoonoses of High Institutions in Anhui, Anhui Medical University, Hefei, China., Zhang C; Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China.; Anhui Province Key Laboratory of Zoonoses, the Key Laboratory of Zoonoses of High Institutions in Anhui, Anhui Medical University, Hefei, China., Chen C; Anhui Province Key Laboratory of Zoonoses, the Key Laboratory of Zoonoses of High Institutions in Anhui, Anhui Medical University, Hefei, China.; Department of Microbiology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China. |
| المصدر: | Microbiology spectrum [Microbiol Spectr] 2024 Jul 02; Vol. 12 (7), pp. e0379223. Date of Electronic Publication: 2024 May 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : ASM Press, 2013- |
| مواضيع طبية MeSH: | Beauveria*/genetics , Beauveria*/pathogenicity , Beauveria*/metabolism , Aedes*/microbiology , Mosquito Control*/methods , Bacillus thuringiensis Toxins*/genetics , Hemolysin Proteins*/genetics , Hemolysin Proteins*/metabolism , Bacillus thuringiensis*/genetics , Bacillus thuringiensis*/metabolism , Endotoxins*/genetics , Endotoxins*/metabolism , Pest Control, Biological*/methods , Larva*/microbiology, Animals ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Virulence/genetics ; Spores, Fungal/genetics ; Insecticides/pharmacology ; Insecticides/metabolism |
| مستخلص: | The entomopathogenic fungus Beauveria bassiana provides an eco-friendly substitute to chemical insecticides for mosquito control. Nevertheless, its widespread application has been hindered by its comparatively slow efficacy in eliminating mosquitoes. To augment the potency of B. bassiana against Aedes mosquitoes, a novel recombinant strain, Bb -Cyt1Aa, was developed by incorporating the Bacillus thuringiensis toxin gene Cyt1Aa into B. bassiana . The virulence of Bb -Cyt1Aa was evaluated against Aedes aegypti and Aedes albopictus using insect bioassays. Compared to the wild-type (WT) strain, the median lethal time (LT Importance: Beauveria bassiana is a naturally occurring fungus that can be utilized as a bioinsecticide against mosquitoes. Cyt1Aa is a delta-endotoxin protein produced by Bacillus thuringiensis that exhibits specific and potent insecticidal activity against mosquitoes. In our study, the expression of this toxin Cyt1Aa in B. bassiana enhances the virulence of B. bassiana against Aedes aegypti and Aedes albopictus , thereby increasing their effectiveness in killing mosquitoes. This novel strain can be used alongside chemical insecticides to reduce dependence on harmful chemicals, thereby minimizing negative impacts on the environment and human health. Additionally, the potential resistance of B. bassiana against mosquitoes in the future could be overcome by acquiring novel combinations of exogenous toxin genes. The presence of B. bassiana that expresses Cyt1Aa is of significant importance in mosquito control as it enhances genetic diversity, creates novel virulent strains, and contributes to the development of safer and more sustainable methods of mosquito control. Competing Interests: The authors declare no conflict of interest. |
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| معلومات مُعتمدة: | 82102432 MOST | National Natural Science Foundation of China (NSFC); 2108085QH347 Anhui Provincial Natural Science Foundation Project |
| فهرسة مساهمة: | Keywords: Aedes aegypti; Aedes albopictus; Beauveria bassiana; Cyt1Aa; mosquito control |
| المشرفين على المادة: | 0 (Bacillus thuringiensis Toxins) 0 (Hemolysin Proteins) 0 (Endotoxins) 0 (insecticidal crystal protein, Bacillus Thuringiensis) 0 (Bacterial Proteins) 0 (Insecticides) |
| تواريخ الأحداث: | Date Created: 20240529 Date Completed: 20240702 Latest Revision: 20240711 |
| رمز التحديث: | 20240711 |
| مُعرف محوري في PubMed: | PMC11218515 |
| DOI: | 10.1128/spectrum.03792-23 |
| PMID: | 38809029 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2165-0497 |
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| DOI: | 10.1128/spectrum.03792-23 |