دورية أكاديمية
أعرض في EDS

First report of V1016I, F1534C and V410L kdr mutations associated with pyrethroid resistance in Aedes aegypti populations from Niamey, Niger.

التفاصيل البيبلوغرافية
العنوان: First report of V1016I, F1534C and V410L kdr mutations associated with pyrethroid resistance in Aedes aegypti populations from Niamey, Niger.
المؤلفون: Maiga AA; Laboratoire d'Entomologie Fondamentale et Appliquée, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso., Sombié A; Laboratoire d'Entomologie Fondamentale et Appliquée, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso., Zanré N; Laboratoire d'Entomologie Fondamentale et Appliquée, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso., Yaméogo F; Laboratoire d'Entomologie Fondamentale et Appliquée, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso., Iro S; Unité de Parasitologie et d'Entomologie Médicale, Centre de Recherche Médicale et Sanitaire, Niamey, Niger., Testa J; Faculté de Médecine, Université Côte d'Azur, Côte d'Azur, France., Sanon A; Laboratoire d'Entomologie Fondamentale et Appliquée, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso., Koita O; Laboratoire de Biologie Moléculaire Appliquée, Université des Sciences, des Techniques et Technologies de Bamako, Bamako, Mali., Kanuka H; Center for Medical Entomology, The Jikei University School of Medicine, Tokyo, Japan.; Department of Tropical Medicine, The Jikei University School of Medicine, Tokyo, Japan., McCall PJ; Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom., Weetman D; Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom., Badolo A; Laboratoire d'Entomologie Fondamentale et Appliquée, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso.
المصدر: PloS one [PLoS One] 2024 May 29; Vol. 19 (5), pp. e0304550. Date of Electronic Publication: 2024 May 29 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Aedes*/genetics , Aedes*/drug effects , Insecticide Resistance*/genetics , Pyrethrins*/pharmacology , Insecticides*/pharmacology , Mutation*, Animals ; Niger ; Mosquito Vectors/genetics ; Mosquito Vectors/drug effects ; Genotype ; Larva/drug effects ; Larva/genetics ; Insect Proteins/genetics ; Insect Proteins/metabolism
مستخلص: Background: Ae. aegypti is the vector of important μ arboviruses, including dengue, Zika, chikungunya and yellow fever. Despite not being specifically targeted by insecticide-based control programs in West Africa, resistance to insecticides in Ae. aegypti has been reported in countries within this region. In this study, we investigated the status and mechanisms of Ae. aegypti resistance in Niamey, the capital of Niger. This research aims to provide baseline data necessary for arbovirus outbreak prevention and preparedness in the country.
Methods: Ovitraps were used to collect Ae. aegypti eggs, which were subsequently hatched in the insectary for bioassay tests. The hatched larvae were then reared to 3-5-day-old adults for WHO tube and CDC bottle bioassays, including synergist tests. The kdr mutations F1534C, V1016I, and V410L were genotyped using allele-specific PCR and TaqMan qPCR methods.
Results: Ae. aegypti from Niamey exhibited moderate resistance to pyrethroids but susceptibility to organophosphates and carbamates. The kdr mutations, F1534C, V1016I and V410L were detected with the resistant tri-locus haplotype 1534C+1016L+410L associated with both permethrin and deltamethrin resistance. Whereas the homozygote tri-locus resistant genotype 1534CC+1016LL+410LL was linked only to permethrin resistance. The involvement of oxidase and esterase enzymes in resistance mechanisms was suggested by partial restoration of mosquitoes' susceptibility to pyrethroids in synergist bioassays.
Conclusion: This study is the first report of Ae. aegypti resistance to pyrethroid insecticides in Niamey. The resistance is underpinned by target site mutations and potentially involves metabolic enzymes. The observed resistance to pyrethroids coupled with susceptibility to other insecticides, provides data to support evidence-based decision-making for Ae. aegypti control in Niger.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Maiga et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
References: Insects. 2018 Feb 14;9(1):. (PMID: 29443870)
Annu Rev Entomol. 2015 Jan 7;60:537-59. (PMID: 25564745)
Lancet. 2006 May 6;367(9521):1473. (PMID: 16683301)
PLoS Negl Trop Dis. 2022 Jul 6;16(7):e0010059. (PMID: 35793379)
Med Vet Entomol. 2022 Sep;36(3):309-319. (PMID: 35869781)
J Travel Med. 2019 Oct 14;26(7):. (PMID: 30407572)
Sci Rep. 2017 Apr 19;7:46549. (PMID: 28422157)
Emerg Infect Dis. 2018 Aug;24(8):1544-1547. (PMID: 30015610)
IJID Reg. 2023 Apr 07;7:230-232. (PMID: 37168517)
Parasit Vectors. 2023 Apr 18;16(1):135. (PMID: 37072865)
PLoS Negl Trop Dis. 2015 Dec 11;9(12):e0004263. (PMID: 26658798)
Nat Microbiol. 2019 Sep;4(9):1508-1515. (PMID: 31182801)
Parasit Vectors. 2022 Feb 19;15(1):61. (PMID: 35183249)
Curr Res Parasitol Vector Borne Dis. 2022;2:100074. (PMID: 35726222)
J Med Entomol. 2019 Apr 16;56(3):811-816. (PMID: 30715464)
Parasit Vectors. 2023 Apr 19;16(1):137. (PMID: 37076920)
PLoS Negl Trop Dis. 2021 May 10;15(5):e0009393. (PMID: 33970904)
Trop Med Health. 2019 Jan 04;47:2. (PMID: 30787670)
BMC Infect Dis. 2019 Aug 28;19(1):750. (PMID: 31455279)
Insects. 2021 Sep 24;12(10):. (PMID: 34680634)
Heliyon. 2020 Jul 14;6(7):e04389. (PMID: 32695907)
PLoS Negl Trop Dis. 2019 May 23;13(5):e0007439. (PMID: 31120874)
PLoS Negl Trop Dis. 2020 May 8;14(5):e0008216. (PMID: 32384079)
J Med Entomol. 2021 Jul 16;58(4):1808-1816. (PMID: 33876233)
Parasit Vectors. 2022 Oct 21;15(1):381. (PMID: 36271451)
Viruses. 2021 May 12;13(5):. (PMID: 34065928)
Malar J. 2008 Sep 25;7:189. (PMID: 18817574)
Sci Rep. 2018 Apr 30;8(1):6747. (PMID: 29712956)
Viruses. 2023 Feb 27;15(3):. (PMID: 36992346)
J Med Entomol. 2022 Sep 14;59(5):1741-1748. (PMID: 35960164)
J Med Entomol. 2020 Jul 4;57(4):1239-1245. (PMID: 32112094)
PLoS Negl Trop Dis. 2021 Jan 19;15(1):e0009084. (PMID: 33465099)
N Engl J Med. 2016 Jul 28;375(4):301-3. (PMID: 27276108)
Heliyon. 2022 May 17;8(5):e09459. (PMID: 35620619)
المشرفين على المادة: 0 (Pyrethrins)
0 (Insecticides)
0 (Insect Proteins)
تواريخ الأحداث: Date Created: 20240529 Date Completed: 20240529 Latest Revision: 20240531
رمز التحديث: 20240531
مُعرف محوري في PubMed: PMC11135682
DOI: 10.1371/journal.pone.0304550
PMID: 38809933
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0304550