دورية أكاديمية
Spectroscopic diagnosis and metabolite characterization of cisplatin resistance regulated by FDFT1 in bladder cancer tissue.
| العنوان: | Spectroscopic diagnosis and metabolite characterization of cisplatin resistance regulated by FDFT1 in bladder cancer tissue. |
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| المؤلفون: | Kanmalar M; Department of Physics, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia., Kamal R; Department of Physics, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia., Abdul Sani SF; Department of Physics, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: s.fairus@um.edu.my., Pathmanathan D; Department of Mathematic, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia., Bm Said NA; Department of Pharmaceutical Life Sciences, Faculty of Pharmacy, University of Malaya, 50603, Kuala Lumpur, Malaysia., Paramanantham Y; School of Pharmacy, Monash University Malaysia, Selangor Darul Ehsan, 47500, Malaysia., Abd Jamil AH; Department of Pharmaceutical Life Sciences, Faculty of Pharmacy, University of Malaya, 50603, Kuala Lumpur, Malaysia., Mun KS; Department of Pathology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia., Kuppusamy S; Department of Surgery, University of Malaya, 50603, Kuala Lumpur, Malaysia., Almugren KS; Department of Physics, College of Science, Princess Nourah bint Abdulrahman University, Saudi Arabia. Electronic address: ksalmogren@pnu.edu.sa., Almajid HF; Medicine College, Alfaisal University, Riyadh, Kingdom of Saudi Arabia., Bradley DA; Sunway University, Centre for Applied Physics and Radiation Technologies, Jalan Universiti, 46150, PJ, Malaysia; School of Mathematics and Physics, University of Surrey, Guildford, GU2 7XH, UK. |
| المصدر: | Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine [Appl Radiat Isot] 2024 Aug; Vol. 210, pp. 111372. Date of Electronic Publication: 2024 May 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 9306253 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-9800 (Electronic) Linking ISSN: 09698043 NLM ISO Abbreviation: Appl Radiat Isot Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford ; New York : Pergamon Press, c1993- |
| مواضيع طبية MeSH: | Cisplatin*/pharmacology , Cisplatin*/therapeutic use , Drug Resistance, Neoplasm* , Spectrum Analysis, Raman*/methods , Urinary Bladder Neoplasms*/drug therapy , Urinary Bladder Neoplasms*/metabolism , Urinary Bladder Neoplasms*/pathology, Humans ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/therapeutic use ; Farnesyltranstransferase/metabolism ; X-Ray Diffraction ; Farnesyl-Diphosphate Farnesyltransferase/metabolism |
| مستخلص: | As is the case for most solid tumours, chemotherapy remains the backbone in the management of metastatic disease. However, the occurrence of chemotherapy resistance is a cause to worry, especially in bladder cancer. Extensive evidence indicates molecular changes in bladder cancer cells to be the underlying cause of chemotherapy resistance, including the reduced expression of farnesyl-diphosphate farnesyltransferase 1 (FDFT1) - a gene involved in cholesterol biosynthesis. This can likely be a hallmark in examining the resistance and sensitivity of chemotherapy drugs. This work performs spectroscopic analysis and metabolite characterization on resistant, sensitive, stable-disease and healthy bladder tissues. Raman spectroscopy has detected peaks at around 1003 cm -1 (squalene), 1178 cm -1 (cholesterol), 1258 cm -1 (cholesteryl ester), 1343 cm -1 (collagen), 1525 cm -1 (carotenoid), 1575 cm -1 (DNA bases) and 1608 cm -1 (cytosine). The peak parameters were examined, and statistical analysis was performed on the peak features, attaining significant differences between the sample groups. Small-angle x-ray scattering (SAXS) measurements observed the triglyceride peak together with 6th, 7th and 8th - order collagen peaks; peak parameters were also determined. Neutron activation analysis (NAA) detected seven trace elements. Carbon (Ca), magnesium (Mg), chlorine (Cl) and sodium (Na) have been found to have the greatest concentration in the sample groups, suggestive of a role as a biomarker for cisplatin resistance studies. Results from the present research are suggested to provide an important insight into understanding the development of drug resistance in bladder cancer, opening up the possibility of novel avenues for treatment through personalised interventions. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bladder cancer tissue; Diagnostic; Neutron activation analysis; Raman spectroscopy; Small-angle X-ray scattering |
| المشرفين على المادة: | 0 (Antineoplastic Agents) Q20Q21Q62J (Cisplatin) EC 2.5.1.29 (Farnesyltranstransferase) EC 2.5.1.21 (Farnesyl-Diphosphate Farnesyltransferase) |
| تواريخ الأحداث: | Date Created: 20240529 Date Completed: 20240611 Latest Revision: 20240621 |
| رمز التحديث: | 20240621 |
| DOI: | 10.1016/j.apradiso.2024.111372 |
| PMID: | 38810354 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-9800 |
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| DOI: | 10.1016/j.apradiso.2024.111372 |