دورية أكاديمية
T-cells and CD45-cells discovery in the central nervous system of healthy and nodavirus-infected teleost fish Dicentrarchuslabrax.
| العنوان: | T-cells and CD45-cells discovery in the central nervous system of healthy and nodavirus-infected teleost fish Dicentrarchuslabrax. |
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| المؤلفون: | Pianese V; University of Tuscia, Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), Largo dell'Università, 01100, Viterbo (I), Italy. Electronic address: valeria.pianese@unitus.it., Alvarez-Torres D; University of Málaga, Institute of Biotecnology and Blue Development (IBYDA), 29071, Málaga (E), Spain. Electronic address: dani-alto@uma.es., Gemez-Mata J; University of Málaga, Institute of Biotecnology and Blue Development (IBYDA), Dept. Microbiology, Faculty of Sciences, 29071, Málaga (E), Spain. Electronic address: juangemez@uma.es., Garcia-Rosado E; University of Málaga, Institute of Biotecnology and Blue Development (IBYDA), Dept. Microbiology, Faculty of Sciences, 29071, Málaga (E), Spain. Electronic address: megarciar@uma.es., Moreno P; University of Málaga, Institute of Biotecnology and Blue Development (IBYDA), Dept. Microbiology, Faculty of Sciences, 29071, Málaga (E), Spain. Electronic address: patriciamgarcia@uma.es., Fausto AM; University of Tuscia, Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), Largo dell'Università, 01100, Viterbo (I), Italy. Electronic address: fausto@unitus.it., Taddei AR; University of Tuscia, Section Microscopy (CGA), Largo dell'Università, 01100, Viterbo (I), Italy. Electronic address: artaddei@unitus.it., Picchietti S; University of Tuscia, Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), Largo dell'Università, 01100, Viterbo (I), Italy. Electronic address: picchietti@unitus.it., Scapigliati G; University of Tuscia, Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), Largo dell'Università, 01100, Viterbo (I), Italy. Electronic address: scapigg@unitus.it. |
| المصدر: | Fish & shellfish immunology [Fish Shellfish Immunol] 2024 Aug; Vol. 151, pp. 109646. Date of Electronic Publication: 2024 May 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 9505220 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9947 (Electronic) Linking ISSN: 10504648 NLM ISO Abbreviation: Fish Shellfish Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; New York : Academic Press, c1991- |
| مواضيع طبية MeSH: | Nodaviridae*/physiology , Fish Diseases*/immunology , Fish Diseases*/virology , Bass*/immunology , RNA Virus Infections*/veterinary , RNA Virus Infections*/immunology , RNA Virus Infections*/virology , Leukocyte Common Antigens*/metabolism , Leukocyte Common Antigens*/genetics , Leukocyte Common Antigens*/immunology , T-Lymphocytes*/immunology, Animals ; Fish Proteins/genetics ; Fish Proteins/immunology ; Central Nervous System/immunology ; Central Nervous System/virology ; Brain/virology ; Brain/immunology |
| مستخلص: | To achieve insights in antiviral immune defense of the central nervous system (CNS), we investigated T cells and CD45 cells in the marine fish model Dicentrarchus labrax infected with the CNS-tropic virus betanodavirus. By employing markers for pan-T cells (mAb DLT15) and CD45-cells (mAb DLT22) in immunofluorescence (IIF) of leukocytes from brain, we obtained 3,7 ± 2.3 % of T cells and 7.3 ± 3.2 % of CD45 + cells. Both IIF and immunoelectron microscopy confirmed a leukocyte/glial morphology for the immunoreactive cells. Quantitative immunohistochemistry (qIHC) of brain/eye sections showed 1.9 ± 0.8 % of T + cells and 2 ± 0.9 % of CD45 + cells in the brain, and 3.6 ± 1.9 % and 4.1 ± 2.2 % in the eye, respectively. After in vivo RGNNV infection the number of T cells/CD45 + leukocytes in the brain increased to 8.3 ± 2.1 % and 11.6 ± 4.4 % (by IIF), and 26.1 ± 3.4 % and 45.6 ± 5.9 % (by qIHC), respectively. In the eye we counted after infection 8.5 ± 4.4 % of T cells and 10.2 ± 5.8 % of CD45 cells. Gene transcription analysis of brain mRNA revealed a strong increase of gene transcripts coding for: antiviral proteins Mx and ISG-12; T-cell related CD3ε/δ, TcRβ, CD4, CD8α, CD45; and for immuno-modulatory cytokines TNFα, IL-2, IL-10. A RAG-1 gene product was also present and upregulated, suggesting somatic recombination in the fish brain. Similar transcription data were obtained in the eye, albeit with differences. Our findings provide first evidence for a recruitment and involvement of T cells and CD45 + leukocytes in the fish eye-brain axis during antiviral responses and suggest similarities in the CNS immune defense across evolutionary distant vertebrates. Competing Interests: Declaration of competing interest Authors declare there are no competing financial interests in relation to the work described. (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Brain antiviral responses; Brain immunity; Brain leukocyte responses; Comparative Immunology; Dicentrarchus labrax; Nodavirus; T cells and CD45 cells |
| المشرفين على المادة: | EC 3.1.3.48 (Leukocyte Common Antigens) 0 (Fish Proteins) |
| تواريخ الأحداث: | Date Created: 20240529 Date Completed: 20240720 Latest Revision: 20240720 |
| رمز التحديث: | 20240721 |
| DOI: | 10.1016/j.fsi.2024.109646 |
| PMID: | 38810712 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-9947 |
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| DOI: | 10.1016/j.fsi.2024.109646 |