دورية أكاديمية
Use of flow cytometry method to detect contaminations of platelet suspensions.
| العنوان: | Use of flow cytometry method to detect contaminations of platelet suspensions. |
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| المؤلفون: | Bolat M; Sakarya University Health Sciences Institute, Sakarya, Turkey., Hatipoğlu H; Medical Microbiology Laboratory, Sakarya Training and Research Hospital, Sakarya, Turkey., Köroğlu M; Faculty of Medicine, Department of Medical Microbiology, Sakarya University, Sakarya, Turkey., Toptan H; Medical Microbiology Laboratory, Sakarya Training and Research Hospital, Sakarya, Turkey. hande_cakar@hotmail.com., Altındiş M; Faculty of Medicine, Department of Medical Microbiology, Sakarya University, Sakarya, Turkey. |
| المصدر: | World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2024 May 30; Vol. 40 (7), pp. 222. Date of Electronic Publication: 2024 May 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 9012472 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-0972 (Electronic) Linking ISSN: 09593993 NLM ISO Abbreviation: World J Microbiol Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2005- : Berlin : Springer Original Publication: Oxford, OX, UK : Published by Rapid Communications of Oxford Ltd in association with UNESCO and in collaboration with the International Union of Microbiological Societies, c1990- |
| مواضيع طبية MeSH: | Blood Safety*/instrumentation , Blood Safety*/methods , Blood Platelets*/microbiology , Flow Cytometry*/standards, Blood Component Removal ; Blood Culture/standards ; Bacteria/isolation & purification ; Humans ; Sensitivity and Specificity |
| مستخلص: | In this study, it was aimed to investigate bacterial contamination in apheresis platelet suspensions (APS) by automated blood culture system and flow cytometry method (FCM).33 spiked APS each using 11 bacterial strains (5 standard strains, 6 clinical isolates), were prepared in three different dilutions (1-10, 10-50, 50-100 cfu/mL), incubated in two different temperatures (35-37 °C and 22-24 °C) and different incubation times (18-96 h) evaluated by FCM. This three different dilutions were also inoculated into special platelet culture bottles (BacT/ALERT® BPA) and loaded into the blood culture system. Additionally 80 APSs routinely prepared in the Transfusion Center were evaluated by both FCM and the blood culture system. Platelets were lysed by freeze-thaw method.All spiked samples were positive with BacT/ALERT® BPA in 12-18 h. In 96 h incubation at 22-24 °C, the presence of bacteria was detected by FCM in all other samples (31/33) except low dilutions (1-10 and 10-100 CFU/ml) of K.pneumoniae standard strain. In the 35-37 °C, the presence of bacteria was detected by FCM in all samples (33/33) after 48 h of incubation. In routine APS one sample detected as positive (Bacillus simplex) with BacT/ALERT® BPA and no positivity was detected by FCM.The freeze-thaw method, which we have optimized for the lysis of platelets, is very practical and can be easily applied. The BacT/ALERT® system has been found to be very sensitive in detecting bacterial contamination in PSs. Flow cytometry method has been found to be successful, fast, easy to use and low cost in detecting bacterial contamination in PSs. (© 2024. The Author(s).) |
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| فهرسة مساهمة: | Keywords: Bacterial contamination of platelet suspension; Blood components; Flow cytometry; Shelf life; Transfusion medicine |
| تواريخ الأحداث: | Date Created: 20240529 Date Completed: 20240529 Latest Revision: 20240623 |
| رمز التحديث: | 20240623 |
| مُعرف محوري في PubMed: | PMC11136822 |
| DOI: | 10.1007/s11274-024-04030-x |
| PMID: | 38811387 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1573-0972 |
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| DOI: | 10.1007/s11274-024-04030-x |