دورية أكاديمية
[Isolation and functional characterization of (iso)eugenol O-methyltransferase (IEMT) gene in Asarum sieboldii].
| العنوان: | [Isolation and functional characterization of (iso)eugenol O-methyltransferase (IEMT) gene in Asarum sieboldii]. |
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| المؤلفون: | Dai RX; School of Pharmacy, Fujian University of Traditional Chinese Medicine Fuzhou 350122, China School of Pharmacy, Naval Medical University Shanghai 200433, China School of Pharmaceutical Sciences, Shanghai Jiao Tong University Shanghai 200240, China., Huang BK; School of Pharmacy, Fujian University of Traditional Chinese Medicine Fuzhou 350122, China School of Pharmacy, Naval Medical University Shanghai 200433, China., Liu Z; School of Pharmaceutical Sciences, Shanghai Jiao Tong University Shanghai 200240, China. |
| المصدر: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2024 May; Vol. 49 (10), pp. 2680-2688. |
| نوع المنشور: | Journal Article; English Abstract |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Country of Publication: China NLM ID: 8913656 Publication Model: Print Cited Medium: Print ISSN: 1001-5302 (Print) Linking ISSN: 10015302 NLM ISO Abbreviation: Zhongguo Zhong Yao Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Original Publication: [Beijing] : Zhongguo yao xue hui : [Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo, 1989- |
| مواضيع طبية MeSH: | Methyltransferases*/genetics , Methyltransferases*/chemistry , Methyltransferases*/metabolism , Eugenol*/analogs & derivatives , Eugenol*/metabolism , Eugenol*/chemistry , Asarum*/genetics , Asarum*/chemistry , Asarum*/enzymology, Plant Proteins/genetics ; Plant Proteins/metabolism ; Plant Proteins/chemistry ; Phylogeny ; Amino Acid Sequence ; Cloning, Molecular |
| مستخلص: | Methyleugenol is one of the main active constituents in the volatile oil of the traditional Chinese medicine Asari Radix et Rhizoma. It possesses various pharmacological effects such as analgesic, anesthetic, and anti-inflammatory properties. In biosynthesis, the initial precursor phenylalanine is finally converted into methyleugenol through a series of intermediate compounds including coniferyl acid, courmaryl acid, caffeic acid, ferulic acid/ferulic-CoA, coniferyl aldehyde, conferyl alcohol, cnfiferyl acetate, and eugenol/isoeugenol, which are produced through catalysis of a large number of enzymes. Eugenol O-methyltransferase(EOMT) is one of the key enzymes in the biosynthesis pathway, capable of methylating eugenol on the para-site hydroxyl group of the benzene ring, thereby generating methyleugenol. Here, an(iso)eugenol O-methyltransferase(IEMT) gene was cloned for the first time from Asarum siebo-ldii, holding an open reading frame that consisted of 1 113 bp and encoded a protein containing 370 amino acid residues. Bioinformatics analysis results showed that this protein was equipped with the characteristic structural domains of methyltransferases such as S-adenosylmethionine(SAM) binding sites and dimerization domains. The prokaryotic expression recombinant plasmid pET28a(+)-AsIEMT was constructed, and the candidate protein was induced and purified. In vitro enzyme assays confirmed that AsIEMT had dual functions. The enzyme could catalyze the production either of methyleugenol from eugenol or of methylisoeugenol from isoeugenol, although the latter was more prevalent. When isoeugenol was used as the substrate, the kinetics parameters K_m and V_(max) of catalytic reaction were(0.90±0.06) mmol·L~(-1) and(1.32±0.04)nmol·s~(-1)·mg~(-1), respectively. This study expanded our understandings of critical enzyme genes involved in phenylpropanoid metabolic pathways, and would facilitate the elucidation of quality formation mechanisms of the TCM Asari Radix et Rhizoma. |
| فهرسة مساهمة: | Keywords: (iso)methyleugenol; Asarum sieboldii; O-methyltransferase; functional characterization |
| المشرفين على المادة: | EC 2.1.1.- (Methyltransferases) 3T8H1794QW (Eugenol) 0 (Plant Proteins) 29T9VA6R7M (methyleugenol) |
| تواريخ الأحداث: | Date Created: 20240530 Date Completed: 20240530 Latest Revision: 20240614 |
| رمز التحديث: | 20240615 |
| DOI: | 10.19540/j.cnki.cjcmm.20240214.103 |
| PMID: | 38812168 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1001-5302 |
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| DOI: | 10.19540/j.cnki.cjcmm.20240214.103 |