دورية أكاديمية
IL-2Rα KO mice exhibit maternal microchimerism and reveal nuclear localization of IL-2Rα in lymphoid and non-lymphoid cells.
| العنوان: | IL-2Rα KO mice exhibit maternal microchimerism and reveal nuclear localization of IL-2Rα in lymphoid and non-lymphoid cells. |
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| المؤلفون: | Wong VA; Department of Neuroscience, Cell Biology, and Physiology, Boonshoft School of Medicine, Wright State University, Dayton, OH, United States., Dinh KN; Fertility Wellness Institute of Ohio West Chester Township, OH, United States., Chen G; Genomics and Microarray Core Facility, University of Texas Southwestern Medical Center Dallas, TX, United States., Wrenshall LE; Department of Neuroscience, Cell Biology, and Physiology, Boonshoft School of Medicine, Wright State University, Dayton, OH, United States.; Department of Medical Education, Boonshoft School of Medicine, Wright State University, Dayton, OH, United States. |
| المصدر: | Frontiers in immunology [Front Immunol] 2024 May 15; Vol. 15, pp. 1369818. Date of Electronic Publication: 2024 May 15 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
| مواضيع طبية MeSH: | Cell Nucleus*/metabolism , Interleukin-2 Receptor alpha Subunit*/metabolism , Interleukin-2 Receptor alpha Subunit*/genetics , Mice, Knockout*, Animals ; Female ; Mice ; Chimerism ; Lymphocytes/immunology ; Lymphocytes/metabolism ; Mice, Inbred C57BL ; Muscle, Smooth, Vascular/metabolism ; Muscle, Smooth, Vascular/cytology ; Muscle, Smooth, Vascular/immunology ; Myocytes, Smooth Muscle/metabolism |
| مستخلص: | Introduction: IL-2Rα knock out (KO) mice have been instrumental to discovering the immunoregulatory properties of IL-2Rα. While initially thought of only as a stimulatory cytokine, IL-2 and IL-2Rα KO mice revealed that this cytokine-receptor system controls immune responses through restimulation-induced cell death and by promoting the survival of T regulatory cells. Although described mostly in the context of lymphocytes, recent studies by our laboratory showed that IL-2R is expressed in smooth muscle cells. Given this finding, we sought to use IL-2Rα KO to determine the function of this receptor in vascular smooth muscle cells. Surprisingly, we found that IL-2Rα KO vascular smooth muscle cells had detectable IL-2Rα. Methods: We used multiple gene and protein-based methods to determine why IL-2Rα KO vascular smooth muscle cells exhibited IL-2Rα protein. These methods included: genomic sequencing, assessing cells and tissues for evidence of maternal microchimerism, and determining the half-life of IL-2Rα protein. Results: Our studies demonstrated the following: (1) in addition to the cell surface, IL-2Rα is localized to the nucleus; (2) the genetic deletion of IL-2Rα is intact in IL-2Rα KO mice; (3) both IL-2Rα KO and WT tissues show evidence of maternal microchimerism, the likely source of IL-2Rα (4) IL-2Rα is transmitted between cells; (5) IL-2Rα has a long half-life; and (6) nuclear IL-2Rα contributes to the regulation of cell proliferation and size. Conclusion: Our findings suggest that the phenotype of complete IL-2Rα loss is more severe than demonstrated by IL-2Rα KO mice, and that IL-2Rα plays a here-to-fore unrecognized role in regulating cell proliferation in non-lymphoid cells. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Wong, Dinh, Chen and Wrenshall.) |
| التعليقات: | Update of: bioRxiv. 2023 Nov 18:2023.11.03.565571. doi: 10.1101/2023.11.03.565571. (PMID: 37961725) |
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| معلومات مُعتمدة: | R03 AG068696 United States AG NIA NIH HHS |
| فهرسة مساهمة: | Keywords: IL-2Rα; cell proliferation; cell size; knock out; maternal microchimerism; nucleus; vascular smooth muscle cells |
| المشرفين على المادة: | 0 (Interleukin-2 Receptor alpha Subunit) 0 (Il2ra protein, mouse) |
| تواريخ الأحداث: | Date Created: 20240530 Date Completed: 20240530 Latest Revision: 20240610 |
| رمز التحديث: | 20240610 |
| مُعرف محوري في PubMed: | PMC11133634 |
| DOI: | 10.3389/fimmu.2024.1369818 |
| PMID: | 38812502 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-3224 |
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| DOI: | 10.3389/fimmu.2024.1369818 |