التفاصيل البيبلوغرافية
| العنوان: |
Glove decontamination procedures to prevent pathogen and DNA cross-contamination among frogs. |
| المؤلفون: |
Noelker JE; Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA., Abreu Ruozzi V; Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA., Craig HM; Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA., Sckrabulis JP; Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA., Raffel TR; Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA. |
| المصدر: |
Diseases of aquatic organisms [Dis Aquat Organ] 2024 May 30; Vol. 158, pp. 173-178. Date of Electronic Publication: 2024 May 30. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: Inter-Research Country of Publication: Germany NLM ID: 8807037 Publication Model: Electronic Cited Medium: Print ISSN: 0177-5103 (Print) Linking ISSN: 01775103 NLM ISO Abbreviation: Dis Aquat Organ Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Oldendorf/Luhe, Federal Republic of Germany : Inter-Research, c1985- |
| مواضيع طبية MeSH: |
Decontamination*/methods , Gloves, Protective*/microbiology, Animals ; Batrachochytrium ; DNA, Fungal ; Mycoses/veterinary ; Mycoses/prevention & control ; Mycoses/microbiology |
| مستخلص: |
Working with aquatic organisms often requires handling multiple individuals in a single session, potentially resulting in cross-contamination by live pathogens or DNA. Most researchers address this problem by disposing of gloves between animals. However, this generates excessive waste and may be impractical for processing very slippery animals that might be easier to handle with cotton gloves. We tested methods to decontaminate cotton or nitrile gloves after contamination with cultured Batrachochytrium dendrobatidis (Bd) or after handling heavily Bd-infected Xenopus laevis with layered cotton and nitrile gloves. Bleach eliminated detectable Bd DNA from culture-contaminated nitrile gloves, but gloves retained detectable Bd DNA following ethanol disinfection. After handling a Bd-infected frog, Bd DNA contamination was greatly reduced by removal of the outer cotton glove, after which either bleach decontamination or ethanol decontamination followed by drying hands with a paper towel lowered Bd DNA below the detection threshold of our assay. These results provide new options to prevent pathogen or DNA cross-contamination, especially when handling slippery aquatic organisms. However, tradeoffs should be considered when selecting an animal handling procedure, such as the potential for cotton gloves to abrade amphibian skin or disrupt skin mucus. Disposing of gloves between animals should remain the gold standard for maintaining biosecurity in sensitive situations. |
| فهرسة مساهمة: |
Keywords: African clawed frog; Chytrid; Chytridiomycosis; Cross-detection; DNA clearance; qPCR assay |
| المشرفين على المادة: |
0 (DNA, Fungal) |
| تواريخ الأحداث: |
Date Created: 20240530 Date Completed: 20240530 Latest Revision: 20240530 |
| رمز التحديث: |
20240530 |
| DOI: |
10.3354/dao03793 |
| PMID: |
38813857 |
| قاعدة البيانات: |
MEDLINE |
