دورية أكاديمية
Flexible DNA Nanoclaws Offer Multivalent and Powerful Spatial Pattern-Recognition for Tumor Cells.
| العنوان: | Flexible DNA Nanoclaws Offer Multivalent and Powerful Spatial Pattern-Recognition for Tumor Cells. |
|---|---|
| المؤلفون: | Chen K; Department of Laboratory Medicine, Zhongshan City People's Hospital, 528403 Zhongshan, Guangdong, China., Mao M; School of Pharmaceutical Sciences, Sun Yat-Sen University, 510006 Guangzhou, Guangdong, China., Huo L; School of Pharmaceutical Sciences, Sun Yat-Sen University, 510006 Guangzhou, Guangdong, China., Wang G; School of Pharmaceutical Sciences, Sun Yat-Sen University, 510006 Guangzhou, Guangdong, China., Pu Z; School of Pharmaceutical Sciences, Sun Yat-Sen University, 510006 Guangzhou, Guangdong, China., Zhang Y; Department of Laboratory Medicine, Zhongshan City People's Hospital, 528403 Zhongshan, Guangdong, China.; School of Pharmaceutical Sciences, Sun Yat-Sen University, 510006 Guangzhou, Guangdong, China. |
| المصدر: | ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2024 Jun 12; Vol. 16 (23), pp. 29760-29769. Date of Electronic Publication: 2024 May 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society |
| مواضيع طبية MeSH: | Aptamers, Nucleotide*/chemistry , Epithelial Cell Adhesion Molecule*/metabolism , Receptor, ErbB-2*/metabolism , DNA*/chemistry, Humans ; Cell Line, Tumor ; Nanostructures/chemistry ; Neoplastic Cells, Circulating/pathology ; Neoplastic Cells, Circulating/metabolism |
| مستخلص: | Multivalent receptor-ligand interactions (RLIs) exhibit excellent affinity for binding when targeting cell membrane receptors with low expression. However, existing strategies only allow for limited control of the valency and spacing of ligands for a certain receptor, lacking recognition patterns for multiple interested receptors with complex spatial distributions. Here, we developed flexible DNA nanoclaws with multivalent aptamers to achieve powerful cell recognition by controlling the spacing of aptamers to match the spatial patterns of receptors. The DNA nanoclaw with spacing-controllable binding sites was constructed via hybrid chain reaction (HCR), enabling dual targeting of HER2 and EpCAM molecules. The results demonstrate that the binding affinity of multivalent DNA nanoclaws to tumor cells is enhanced. We speculate that the flexible structure may conform better to irregularly shaped membrane surfaces, increasing the probability of intermolecular contact. The capture efficiency of circulating tumor cells successfully verified the high affinity and selectivity of this spatial pattern. This strategy will further promote the potential application of DNA frameworks in future disease diagnosis and treatment. |
| فهرسة مساهمة: | Keywords: DNA nanotechnology; cell affinity; cell enrichment; receptor−ligand interactions; tetrahedral framework nucleic acids |
| المشرفين على المادة: | 0 (Aptamers, Nucleotide) 0 (Epithelial Cell Adhesion Molecule) EC 2.7.10.1 (Receptor, ErbB-2) 9007-49-2 (DNA) 0 (EPCAM protein, human) EC 2.7.10.1 (ERBB2 protein, human) |
| تواريخ الأحداث: | Date Created: 20240530 Date Completed: 20240612 Latest Revision: 20240612 |
| رمز التحديث: | 20240612 |
| DOI: | 10.1021/acsami.4c03382 |
| PMID: | 38813974 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1944-8252 |
|---|---|
| DOI: | 10.1021/acsami.4c03382 |