Cobalt Phthalocyanine-Modified Magnetic Metal-Organic Frameworks for Specific Enrichment of Phosphopeptides.

التفاصيل البيبلوغرافية
العنوان:	Cobalt Phthalocyanine-Modified Magnetic Metal-Organic Frameworks for Specific Enrichment of Phosphopeptides.
المؤلفون:	Jiang D; College of Chemistry and Materials Science, Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), Inner Mongolia Minzu University, Tongliao 028000, P. R. China., Wu S; College of Chemistry and Materials Science, Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), Inner Mongolia Minzu University, Tongliao 028000, P. R. China., Li Y; College of Chemistry and Materials Science, Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), Inner Mongolia Minzu University, Tongliao 028000, P. R. China., Qi R; College of Chemistry and Materials Science, Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), Inner Mongolia Minzu University, Tongliao 028000, P. R. China., Liu J; College of Chemistry and Materials Science, Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), Inner Mongolia Minzu University, Tongliao 028000, P. R. China.
المصدر:	ACS biomaterials science & engineering [ACS Biomater Sci Eng] 2024 Jun 10; Vol. 10 (6), pp. 3739-3746. Date of Electronic Publication: 2024 May 30.
نوع المنشور:	Journal Article; Research Support, Non-U.S. Gov't
اللغة:	English
بيانات الدورية:	Publisher: American Chemical Society Country of Publication: United States NLM ID: 101654670 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2373-9878 (Electronic) Linking ISSN: 23739878 NLM ISO Abbreviation: ACS Biomater Sci Eng Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Washington, DC : American Chemical Society, [2015]-
مواضيع طبية MeSH:	Metal-Organic Frameworks/chemistry , Indoles/chemistry , Organometallic Compounds/chemistry , Phosphopeptides/chemistry , Phosphopeptides/isolation & purification , Phosphopeptides/analysis, Humans ; A549 Cells
مستخلص:	For mass spectrometry (MS)-based phosphoproteomics studies, sample pretreatment is an essential step for efficient identification of low-abundance phosphopeptides. Herein, a cobalt phthalocyanine-modified magnetic metal-organic framework (MOF) (Fe 3 O 4 @MIL-101-CoPc) was prepared and applied to enrich phosphopeptides before MS analysis. Fe 3 O 4 @MIL-101-CoPc exhibited an excellent magnetic response (74.98 emu g ^-1 ) and good hydrophilicity (7.75°), which were favorable for the enrichment. Fe 3 O 4 @MIL-101-CoPc showed good enrichment performance with high selectivity (1:1:5000), sensitivity (0.1 fmol), reusability (10 circles), and recovery (91.3%). Additionally, the Fe 3 O 4 @MIL-101-CoPc-based MS method was able to successfully detect 827 phosphopeptides from the A549 cell lysate, demonstrating a high enrichment efficiency (89.3%). This study promotes the application of postfunctionalized MOFs for phosphoproteomics analysis.
فهرسة مساهمة:	Keywords: cobalt phthalocyanine; magnetic; metal−organic framework; nanocomposite; phosphopeptide enrichment
المشرفين على المادة:	0 (Metal-Organic Frameworks) 0 (Indoles) 0 (Organometallic Compounds) 0 (Phosphopeptides) 3317-67-7 (cobalt phthalocyanine)
تواريخ الأحداث:	Date Created: 20240530 Date Completed: 20240610 Latest Revision: 20240612
رمز التحديث:	20240613
DOI:	10.1021/acsbiomaterials.4c00509
PMID:	38814242
قاعدة البيانات:	MEDLINE

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تدمد:	2373-9878
DOI:	10.1021/acsbiomaterials.4c00509