دورية أكاديمية
أعرض في EDS

Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production.

التفاصيل البيبلوغرافية
العنوان: Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production.
المؤلفون: Silva LMG; Postgraduate Program in Medicine, Universidade Nove de Julho, UNINOVE, São Paulo, Brazil., Gouveia VA; Postgraduate Program in Medicine-Biophotonics, Universidade Nove de Julho, UNINOVE, São Paulo, Brazil., Campos GRS; Postgraduate Program in Medicine, Universidade Nove de Julho, UNINOVE, São Paulo, Brazil., Dale CS; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, São Paulo, Brazil., da Palma RK; Facultad De Ciencias De la Salud de Manresa, Universitat de Vic-Universitat Central De Catalunya (UVic-UCC), Barcelona, Spain.; Tissue Repair and Regeneration Laboratory (TR2Lab), Institute for Research and Innovation in Life and Health Sciences in Central Catalonia (Iris-CC). Vic, Spain., de Oliveira APL; Postgraduate Program in Medicine-Biophotonics, Universidade Nove de Julho, UNINOVE, São Paulo, Brazil., Marcos RL; Postgraduate Program in Medicine-Biophotonics, Universidade Nove de Julho, UNINOVE, São Paulo, Brazil., Duran CCG; Postgraduate Program in Medicine, Universidade Nove de Julho, UNINOVE, São Paulo, Brazil.; Postgraduate Program in Medicine-Biophotonics, Universidade Nove de Julho, UNINOVE, São Paulo, Brazil., Cogo JC; Programa de Mestrado em Bioengenharia do Instituto de Ciências e Tecnologia da Universidade Brasil, São Paulo, Brazil., Silva Junior JA; Postgraduate Program in Medicine, Universidade Nove de Julho, UNINOVE, São Paulo, Brazil.; Postgraduate Program in Medicine-Biophotonics, Universidade Nove de Julho, UNINOVE, São Paulo, Brazil., Zamuner SR; Postgraduate Program in Medicine, Universidade Nove de Julho, UNINOVE, São Paulo, Brazil.; Postgraduate Program in Medicine-Biophotonics, Universidade Nove de Julho, UNINOVE, São Paulo, Brazil.
المصدر: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2024 May 30; Vol. 18 (5), pp. e0012227. Date of Electronic Publication: 2024 May 30 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Bothrops* , Myoblasts*/drug effects , Myoblasts*/radiation effects , Myoblasts*/metabolism , Low-Level Light Therapy*/methods , Cytokines*/metabolism , Crotalid Venoms*/toxicity , Myogenin*/metabolism , Myogenin*/genetics, Animals ; Mice ; Cell Line ; PAX7 Transcription Factor/metabolism ; PAX7 Transcription Factor/genetics ; NF-kappa B/metabolism ; MyoD Protein/metabolism ; MyoD Protein/genetics ; Cell Movement/drug effects ; Cell Movement/radiation effects ; Myogenic Regulatory Factor 5/metabolism ; Myogenic Regulatory Factor 5/genetics ; p38 Mitogen-Activated Protein Kinases/metabolism ; Snake Bites/radiotherapy ; Venomous Snakes
مستخلص: Background: Photobiomodulation has exhibited promise in mitigating the local effects induced by Bothrops snakebite envenoming; however, the mechanisms underlying this protection are not yet fully understood. Herein, the effectiveness of photobiomodulation effects on regenerative response of C2C12 myoblast cells following exposure to Bothrops jararacussu venom (BjsuV), as well as the mechanisms involved was investigated.
Methodology/principal Findings: C2C12 myoblast cells were exposed to BjsuV (12.5 μg/mL) and irradiated once for 10 seconds with laser light of 660 nm (14.08 mW; 0.04 cm2; 352 mW/cm2) or 780 nm (17.6 mW; 0.04 cm2; 440 mW/ cm2) to provide energy densities of 3.52 and 4.4 J/cm2, and total energies of 0.1408 and 0.176 J, respectively. Cell migration was assessed through a wound-healing assay. The expression of MAPK p38-α, NF-Кβ, Myf5, Pax-7, MyoD, and myogenin proteins were assessed by western blotting analysis. In addition, interleukin IL1-β, IL-6, TNF-alfa and IL-10 levels were measured in the supernatant by ELISA. The PBM applied to C2C12 cells exposed to BjsuV promoted cell migration, increase the expression of myogenic factors (Pax7, MyF5, MyoD and myogenin), reduced the levels of proinflammatory cytokines, IL1-β, IL-6, TNF-alfa, and increased the levels of anti-inflammatory cytokine IL-10. In addition, PBM downregulates the expression of NF-kB, and had no effect on p38 MAKP.
Conclusion/significance: These data demonstrated that protection of the muscle cell by PBM seems to be related to the increase of myogenic factors as well as the modulation of inflammatory mediators. PBM therapy may offer a new therapeutic strategy to address the local effects of snakebite envenoming by promoting muscle regeneration and reducing the inflammatory process.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: 0 (Cytokines)
0 (Crotalid Venoms)
0 (Myogenin)
0 (PAX7 Transcription Factor)
0 (NF-kappa B)
0 (MyoD Protein)
0 (Pax7 protein, mouse)
0 (Myogenic Regulatory Factor 5)
0 (Myog protein, mouse)
EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SCR Organism: Bothrops jararacussu
تواريخ الأحداث: Date Created: 20240530 Date Completed: 20240621 Latest Revision: 20240623
رمز التحديث: 20240623
مُعرف محوري في PubMed: PMC11192417
DOI: 10.1371/journal.pntd.0012227
PMID: 38814992
قاعدة البيانات: MEDLINE
الوصف
تدمد:1935-2735
DOI:10.1371/journal.pntd.0012227