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Quantum Dot-Fab' Conjugates as Compact Immunolabels for Microtubule Imaging and Cell Classification.

التفاصيل البيبلوغرافية
العنوان: Quantum Dot-Fab' Conjugates as Compact Immunolabels for Microtubule Imaging and Cell Classification.
المؤلفون: Kolossov VL; Department of Bioengineering, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.; Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.; Holonyak Micro and Nanotechnology Laboratory, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States., Kanakaraju K; Department of Bioengineering, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States., Sarkar S; Department of Bioengineering, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.; Holonyak Micro and Nanotechnology Laboratory, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.; Department of Chemistry, Indian Institute of Technology Jodhpur, Rajasthan 342037, India., Arogundade OH; Department of Bioengineering, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.; Holonyak Micro and Nanotechnology Laboratory, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States., Kuo CW; Department of Bioengineering, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.; Holonyak Micro and Nanotechnology Laboratory, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States., Mara NR; Department of Bioengineering, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States., Smith AM; Department of Bioengineering, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.; Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.; Holonyak Micro and Nanotechnology Laboratory, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.; Department of Materials Science and Engineering, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.; Carle Illinois College of Medicine, Urbana, Illinois 61801, United States.
المصدر: ACS nano [ACS Nano] 2024 Jun 11; Vol. 18 (23), pp. 15084-15095. Date of Electronic Publication: 2024 May 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101313589 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1936-086X (Electronic) Linking ISSN: 19360851 NLM ISO Abbreviation: ACS Nano Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington D.C. : American Chemical Society
مواضيع طبية MeSH: Quantum Dots*/chemistry , Immunoglobulin Fab Fragments*/chemistry , Immunoglobulin Fab Fragments*/immunology , Microtubules*/chemistry , Microtubules*/metabolism, Humans ; Animals ; Mice ; Fluorescent Dyes/chemistry
مستخلص: Antibodies and their conjugates of fluorescent labels are widely applied in life sciences research and clinical pathology. Among diverse label types, compact quantum dots (QDs) provide advantages of multispectral multiplexing, bright signals in the deep red and infrared, and low steric hindrance. However, QD-antibody conjugates have random orientation of the antigen-binding domain which may interfere with labeling and are large (20-30 nm) and heterogeneous, which limits penetration into biospecimens. Here, we develop conjugates of compact QDs and Fab' antibody fragments as primary immunolabels. Fab' fragments are conjugated site-specifically through sulfhydryl groups distal to antigen-binding domains, and the multivalent conjugates have small and homogeneous sizes (∼12 nm) near those of full-sized antibodies. Their performance as immunolabels for intracellular antigens is evaluated quantitatively by metrics of microtubule labeling density and connectivity in fixed cells and for cytological identification in fixed brain specimens, comparing results with probes based on spectrally-matched dyes. QD-Fab' conjugates outperformed QD conjugates of full-sized antibodies and could be imaged with bright signals with 1-photon and 2-photon excitation. The results demonstrate a requirement for smaller bioaffinity agents and site-specific orientation for the success of nanomaterial-based labels to enhance penetration in biospecimens and minimize nonspecific staining.
References: J Am Chem Soc. 2016 Mar 16;138(10):3382-94. (PMID: 26863113)
ACS Omega. 2017 Feb 28;2(2):563-572. (PMID: 30023612)
Biosens Bioelectron. 2013 Dec 15;50:460-71. (PMID: 23911661)
Science. 1998 Sep 25;281(5385):2016-8. (PMID: 9748158)
ACS Nano. 2020 Mar 24;14(3):3227-3241. (PMID: 32105448)
Biochem Biophys Res Commun. 2011 Jun 10;409(3):577-82. (PMID: 21609713)
ACS Nano. 2021 Nov 23;15(11):18374-18384. (PMID: 34694776)
Chem Commun (Camb). 2016 Dec 13;52(100):14423-14425. (PMID: 27901526)
Beilstein J Nanotechnol. 2017 Jun 9;8:1238-1249. (PMID: 28685124)
Biomark Res. 2021 Dec 4;9(1):87. (PMID: 34863296)
J Am Chem Soc. 2008 Aug 27;130(34):11278-9. (PMID: 18680294)
Nanomedicine. 2016 Jul;12(5):1421-30. (PMID: 26945976)
ACS Omega. 2017 Sep 30;2(9):5785-5791. (PMID: 30023752)
Science. 2005 Jan 28;307(5709):538-44. (PMID: 15681376)
Clin Exp Immunol. 2008 Jan;151(1):42-50. (PMID: 18005364)
Nat Rev Neurosci. 2015 Feb;16(2):79-93. (PMID: 25601779)
Nat Rev Mol Cell Biol. 2022 Aug;23(8):541-558. (PMID: 35383336)
Nat Biomed Eng. 2017;1:. (PMID: 29119058)
Bioconjug Chem. 2016 Jan 20;27(1):238-46. (PMID: 26700095)
Chem Mater. 2021 Jul 13;33(13):4877-4889. (PMID: 35221487)
Nat Commun. 2013;4:1619. (PMID: 23511483)
Methods Mol Med. 2000;40:325-31. (PMID: 21337103)
J Am Chem Soc. 2010 Jan 20;132(2):472-83. (PMID: 20025223)
Langmuir. 2012 Feb 7;28(5):2761-72. (PMID: 22201293)
Curr Protoc Neurosci. 2020 Jun;92(1):e96. (PMID: 32497404)
Science. 2003 May 30;300(5624):1434-6. (PMID: 12775841)
Chem Mater. 2022 May 24;34(10):4621-4632. (PMID: 36968145)
Methods Mol Biol. 2020;2064:147-158. (PMID: 31565773)
J Neuropathol Exp Neurol. 1993 Nov;52(6):655-66. (PMID: 8229085)
Sci Rep. 2017 Sep 12;7(1):11379. (PMID: 28900238)
Front Immunol. 2014 Oct 20;5:520. (PMID: 25368619)
MAbs. 2012 Jan-Feb;4(1):17-23. (PMID: 22327427)
Nat Biotechnol. 2009 Aug;27(8):732-3. (PMID: 19668181)
Colloids Surf B Biointerfaces. 2023 Apr;224:113192. (PMID: 36791518)
Coord Chem Rev. 2016 Aug 1;320-321:216-237. (PMID: 28344357)
Chem Sci. 2012 Jun 1;3(6):2028-2034. (PMID: 24932403)
Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22287-92. (PMID: 20018714)
Nanomedicine. 2012 May;8(4):516-25. (PMID: 21839049)
Nat Rev Mol Cell Biol. 2020 Jun;21(6):307-326. (PMID: 32107477)
Semin Cell Dev Biol. 2023 Mar 15;137:87-95. (PMID: 35915025)
PLoS One. 2016 Sep 29;11(9):e0163856. (PMID: 27685858)
Anal Biochem. 2006 Jun 15;353(2):204-8. (PMID: 16564018)
Anal Bioanal Chem. 2012 Sep;404(5):1449-63. (PMID: 22797718)
Diagnostics (Basel). 2017 Oct 17;7(4):. (PMID: 29039819)
Bioconjug Chem. 2014 Dec 17;25(12):2205-11. (PMID: 25397889)
Cell. 2010 Jan 8;140(1):74-87. (PMID: 20074521)
Bioconjug Chem. 2016 Oct 19;27(10):2260-2265. (PMID: 27631603)
Nano Rev. 2012;3:. (PMID: 22833780)
Biointerphases. 2017 Mar 16;12(2):02D301. (PMID: 28301944)
J Immunol Methods. 1983 Nov 11;64(1-2):141-6. (PMID: 6644029)
J Cell Biol. 2015 Jun 8;209(5):633-44. (PMID: 26056137)
Nat Commun. 2019 Feb 22;10(1):909. (PMID: 30796217)
Colloids Surf B Biointerfaces. 2018 Jul 1;167:267-274. (PMID: 29677598)
Chem Sci. 2017 Jan 1;8(1):63-77. (PMID: 28451149)
PLoS One. 2013 May 22;8(5):e64320. (PMID: 23717596)
J Am Soc Mass Spectrom. 2009 Dec;20(12):2258-64. (PMID: 19786356)
Science. 1998 Sep 25;281(5385):2013-6. (PMID: 9748157)
Langmuir. 2012 Oct 30;28(43):15177-84. (PMID: 23006042)
J Phys Chem B. 2012 Sep 13;116(36):11370-8. (PMID: 22900542)
Nat Commun. 2015 Oct 05;6:8210. (PMID: 26437175)
Bioconjug Chem. 2005 Sep-Oct;16(5):1282-90. (PMID: 16173809)
Int J Mol Sci. 2021 Feb 12;22(4):. (PMID: 33673348)
ACS Nano. 2020 Jul 28;14(7):8343-8358. (PMID: 32525656)
Bioconjug Chem. 2014 Jul 16;25(7):1272-81. (PMID: 24936983)
Mod Pathol. 2006 Sep;19(9):1181-91. (PMID: 16778828)
Neuroscience. 2021 May 10;462:4-21. (PMID: 32554107)
J Protein Chem. 1988 Apr;7(2):165-71. (PMID: 3255367)
Nat Methods. 2005 Oct;2(10):743-9. (PMID: 16179920)
Nat Commun. 2018 Oct 26;9(1):4461. (PMID: 30367061)
معلومات مُعتمدة: R01 CA227699 United States CA NCI NIH HHS; R01 GM131272 United States GM NIGMS NIH HHS; R01 NS100019 United States NS NINDS NIH HHS; R01 NS097610 United States NS NINDS NIH HHS; R01 EB032249 United States EB NIBIB NIH HHS; R01 EB032725 United States EB NIBIB NIH HHS; T32 EB019944 United States EB NIBIB NIH HHS
فهرسة مساهمة: Keywords: cytoskeleton; immunofluorescence; nanocrystal; neuron; neuroscience; spatial proteomics
المشرفين على المادة: 0 (Immunoglobulin Fab Fragments)
0 (Fluorescent Dyes)
تواريخ الأحداث: Date Created: 20240530 Date Completed: 20240611 Latest Revision: 20240724
رمز التحديث: 20240725
مُعرف محوري في PubMed: PMC11262708
DOI: 10.1021/acsnano.4c02215
PMID: 38815170
قاعدة البيانات: MEDLINE
الوصف
تدمد:1936-086X
DOI:10.1021/acsnano.4c02215