دورية أكاديمية
Whole-genome sequencing, multilocus sequence typing, and resistance mechanism of the carbapenem-resistant Pseudomonas aeruginosa in China.
| العنوان: | Whole-genome sequencing, multilocus sequence typing, and resistance mechanism of the carbapenem-resistant Pseudomonas aeruginosa in China. |
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| المؤلفون: | Zhao X; Department of Clinical Laboratory, Weifang People's Hospital, Weifang, Shandong Province, China., Qin J; Department of Clinical Laboratory, Weifang People's Hospital, Weifang, Shandong Province, China., Chen G; Department of Clinical Laboratory, Weifang People's Hospital, Weifang, Shandong Province, China., Yang C; The Center for Microbes, Development and Health, CAS Key Laboratory of Molecular Virology and Immunology, Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, Shanghai, China., Wei J; Department of Clinical Laboratory, Weifang People's Hospital, Weifang, Shandong Province, China., Li W; Department of Clinical Laboratory, Weifang People's Hospital, Weifang, Shandong Province, China., Jia W; Department of Clinical Laboratory, Weifang People's Hospital, Weifang, Shandong Province, China. Electronic address: wfrywsw@163.com. |
| المصدر: | Microbial pathogenesis [Microb Pathog] 2024 Jul; Vol. 192, pp. 106720. Date of Electronic Publication: 2024 May 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; Orlando : Academic Press, c1986- |
| مواضيع طبية MeSH: | Pseudomonas aeruginosa*/genetics , Pseudomonas aeruginosa*/drug effects , Pseudomonas aeruginosa*/classification , Pseudomonas aeruginosa*/isolation & purification , Multilocus Sequence Typing* , Whole Genome Sequencing* , Carbapenems*/pharmacology , Anti-Bacterial Agents*/pharmacology , Bacterial Proteins*/genetics , Microbial Sensitivity Tests* , Pseudomonas Infections*/microbiology , beta-Lactamases*/genetics, China ; Humans ; Retrospective Studies ; Porins/genetics ; Genome, Bacterial/genetics ; Membrane Transport Proteins/genetics ; Tertiary Care Centers ; Bacterial Outer Membrane Proteins/genetics |
| مستخلص: | Pseudomonas aeruginosa is a significant pathogen responsible for severe multisite infections with high morbidity and mortality rates. This study analyzed carbapenem-resistant Pseudomonas aeruginosa (CRPA) at a tertiary hospital in Shandong, China, using whole-genome sequencing (WGS). The objective was to explore the mechanisms and molecular characteristics of carbapenem resistance. A retrospective analysis of 91 isolates from January 2022 to March 2023 was performed, which included strain identification and antimicrobial susceptibility testing. WGS was utilized to determine the genome sequences of these CRPA strains, and the species were precisely identified using average nucleotide identification (ANI), with further analysis on multilocus sequence typing and strain relatedness. Some strains were found to carry the ampD and oprD genes, while only a few harbored carbapenemase genes or related genes. Notably, all strains possessed the mexA, mexE, and mexX genes. The major lineage identified was ST244, followed by ST235. The study revealed a diverse array of carbapenem resistance mechanisms among hospital isolates, differing from previous studies in mainland China. It highlighted that carbapenem resistance is not due to a single mechanism but rather a combination of enzyme-mediated resistance, AmpC overexpression, OprD dysfunction, and efflux pump overexpression. This research provides valuable insights into the evolutionary mechanisms and molecular features of CRPA resistance in this region, aiding in the national prevention and control of CRPA, and offering references for targeting and developing new drugs. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Carbapenem resistance; Efflux pumps; Multilocus sequence typing; OprD; Pseudomonas aeruginosa; Whole-genome sequencing |
| المشرفين على المادة: | 0 (Carbapenems) 0 (Anti-Bacterial Agents) 0 (Bacterial Proteins) EC 3.5.2.6 (beta-Lactamases) 148412-32-2 (OprD protein, Pseudomonas aeruginosa) EC 3.5.2.6 (carbapenemase) 0 (Porins) 0 (Membrane Transport Proteins) 0 (Bacterial Outer Membrane Proteins) 0 (MexA protein, Pseudomonas aeruginosa) |
| تواريخ الأحداث: | Date Created: 20240530 Date Completed: 20240615 Latest Revision: 20240615 |
| رمز التحديث: | 20240616 |
| DOI: | 10.1016/j.micpath.2024.106720 |
| PMID: | 38815778 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-1208 |
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| DOI: | 10.1016/j.micpath.2024.106720 |