دورية أكاديمية
Adaptor protein 14-3-3zeta promotes corneal wound healing via regulating cell homeostasis, a potential novel therapy for corneal injury.
| العنوان: | Adaptor protein 14-3-3zeta promotes corneal wound healing via regulating cell homeostasis, a potential novel therapy for corneal injury. |
|---|---|
| المؤلفون: | Jiang Y; Medical School of Chinese PLA, Beijing, 100089, China; Department of Ophthalmology, The Third Medical Center, Chinese PLA General Hospital, Beijing, 100089, China; National Key Laboratory of Kidney Diseases, Chinese PLA General Hospital, Beijing, 100089, China., Chen M; School of Medicine, Nankai University, Tianjin, 300071, China; National Key Laboratory of Kidney Diseases, Chinese PLA General Hospital, Beijing, 100089, China., Xu N; Medical School of Chinese PLA, Beijing, 100089, China; Department of Ophthalmology, The Third Medical Center, Chinese PLA General Hospital, Beijing, 100089, China; National Key Laboratory of Kidney Diseases, Chinese PLA General Hospital, Beijing, 100089, China., Li Z; Medical School of Chinese PLA, Beijing, 100089, China; Department of Ophthalmology, The Third Medical Center, Chinese PLA General Hospital, Beijing, 100089, China; National Key Laboratory of Kidney Diseases, Chinese PLA General Hospital, Beijing, 100089, China., Li X; Medical School of Chinese PLA, Beijing, 100089, China; Department of Ophthalmology, The Third Medical Center, Chinese PLA General Hospital, Beijing, 100089, China; National Key Laboratory of Kidney Diseases, Chinese PLA General Hospital, Beijing, 100089, China., Yu H; Medical School of Chinese PLA, Beijing, 100089, China; Department of Ophthalmology, The Third Medical Center, Chinese PLA General Hospital, Beijing, 100089, China; National Key Laboratory of Kidney Diseases, Chinese PLA General Hospital, Beijing, 100089, China., Sun J; Medical School of Chinese PLA, Beijing, 100089, China; Department of Ophthalmology, The Third Medical Center, Chinese PLA General Hospital, Beijing, 100089, China; National Key Laboratory of Kidney Diseases, Chinese PLA General Hospital, Beijing, 100089, China., Wang A; Medical School of Chinese PLA, Beijing, 100089, China; Department of Ophthalmology, The Third Medical Center, Chinese PLA General Hospital, Beijing, 100089, China; National Key Laboratory of Kidney Diseases, Chinese PLA General Hospital, Beijing, 100089, China., Huang Y; Department of Ophthalmology, The Third Medical Center, Chinese PLA General Hospital, Beijing, 100089, China., Wang L; Department of Ophthalmology, The Third Medical Center, Chinese PLA General Hospital, Beijing, 100089, China; School of Medicine, Nankai University, Tianjin, 300071, China; National Key Laboratory of Kidney Diseases, Chinese PLA General Hospital, Beijing, 100089, China. Electronic address: liqiangw@301hospital.com.cn. |
| المصدر: | Experimental eye research [Exp Eye Res] 2024 Jul; Vol. 244, pp. 109948. Date of Electronic Publication: 2024 May 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 0370707 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0007 (Electronic) Linking ISSN: 00144835 NLM ISO Abbreviation: Exp Eye Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Academic Press Original Publication: London. |
| مواضيع طبية MeSH: | 14-3-3 Proteins*/metabolism , 14-3-3 Proteins*/genetics , 14-3-3 Proteins*/biosynthesis , Burns, Chemical*/metabolism , Burns, Chemical*/pathology , Burns, Chemical*/drug therapy , Corneal Injuries*/metabolism , Corneal Injuries*/pathology , Corneal Injuries*/genetics , Wound Healing*/drug effects , Wound Healing*/physiology, Animals ; Humans ; Male ; Mice ; Blotting, Western ; Cell Movement ; Cell Proliferation ; Cells, Cultured ; Disease Models, Animal ; Epithelium, Corneal/metabolism ; Epithelium, Corneal/drug effects ; Epithelium, Corneal/injuries ; Eye Burns/chemically induced ; Gene Expression Regulation ; Homeostasis ; Mice, Inbred C57BL ; Sodium Hydroxide |
| مستخلص: | Severe corneal injury can lead to blindness even after prompt treatment. 14-3-3zeta, a member of an adaptor protein family, contributes to tissue repair by enhancing cellular viability and inhibiting fibrosis and inflammation in renal disease or arthritis. However, its role in corneal regeneration is less studied. In this study, filter disc of 2-mm diameter soaked in sodium hydroxide with a concentration of 0.5 N was placed at the center of the cornea for 30 s to establish a mouse model of corneal alkali injury. We found that 14-3-3zeta, which is mainly expressed in the epithelial layer, was upregulated following injury. Overexpression of 14-3-3zeta in ocular tissues via adeno-associated virus-mediated subconjunctival delivery promoted corneal wound healing, showing improved corneal structure and transparency. In vitro studies on human corneal epithelial cells showed that 14-3-3zeta was critical for cell proliferation and migration. mRNA-sequencing in conjunction with KEGG analysis and validation experiments revealed that 14-3-3zeta regulated the mRNA levels of ITGB1, PIK3R1, FGF5, PRKAA1 and the phosphorylation level of Akt, suggesting the involvement of the PI3K-Akt pathway in 14-3-3zeta-mediated tissue repair. 14-3-3zeta is a potential novel therapeutic candidate for treating severe corneal injury. (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 14-3-3zeta; Alkali burn; Corneal epithelial cells; Gene therapy; Wound healing |
| المشرفين على المادة: | 0 (14-3-3 Proteins) 55X04QC32I (Sodium Hydroxide) |
| تواريخ الأحداث: | Date Created: 20240530 Date Completed: 20240614 Latest Revision: 20240716 |
| رمز التحديث: | 20240716 |
| DOI: | 10.1016/j.exer.2024.109948 |
| PMID: | 38815790 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-0007 |
|---|---|
| DOI: | 10.1016/j.exer.2024.109948 |