دورية أكاديمية
GRSF1 deficiency attenuates mitochondrial function in aging granulosa cells.
| العنوان: | GRSF1 deficiency attenuates mitochondrial function in aging granulosa cells. |
|---|---|
| المؤلفون: | Wen C; Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.; State Key Laboratory of Reproductive Medicine and Offspring Health, Center for Reproductive Medicine, Institute of Women, Children and Reproductive Health,Shandong University, Jinan, Shandong, China.; Guangdong Provincial Clinical Research Center for Obstetrical and Gynecological Diseases, Guangzhou, China., Jiang L; Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.; Guangdong Provincial Clinical Research Center for Obstetrical and Gynecological Diseases, Guangzhou, China., Pan P; Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.; Guangdong Provincial Clinical Research Center for Obstetrical and Gynecological Diseases, Guangzhou, China., Huang J; Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.; Guangdong Provincial Clinical Research Center for Obstetrical and Gynecological Diseases, Guangzhou, China., Xie Y; Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.; Guangdong Provincial Clinical Research Center for Obstetrical and Gynecological Diseases, Guangzhou, China., Ou S; Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.; Guangdong Provincial Clinical Research Center for Obstetrical and Gynecological Diseases, Guangzhou, China., Li Y; Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.; Guangdong Provincial Clinical Research Center for Obstetrical and Gynecological Diseases, Guangzhou, China. |
| المصدر: | Reproduction (Cambridge, England) [Reproduction] 2024 Jun 21; Vol. 168 (2). Date of Electronic Publication: 2024 Jun 21 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published for the Society for Reproduction and Fertility by BioScientifica Country of Publication: England NLM ID: 100966036 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1741-7899 (Electronic) Linking ISSN: 14701626 NLM ISO Abbreviation: Reproduction Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2004->: Bristol, UK : Published for the Society for Reproduction and Fertility by BioScientifica Original Publication: Cambridge, UK : Journals of Reproduction and Fertility, Ltd. c2001- |
| مواضيع طبية MeSH: | Granulosa Cells*/metabolism , Mitochondria*/metabolism , Reactive Oxygen Species*/metabolism , Oxidative Stress* , Aging*/metabolism , Cellular Senescence*, Female ; Humans ; Adult ; Superoxide Dismutase/metabolism ; Superoxide Dismutase/genetics ; Cells, Cultured ; Poly(A)-Binding Proteins |
| مستخلص: | In Brief: Ovarian aging results in reactive oxygen species accumulation and mitochondrial deterioration. During the aging process, GRSF1 deficiency attenuates mitochondrial function in aging granulosa cells. Abstract: Ovarian aging critically influences reproductive potential, with a marked decrease in oocyte quality and quantity and an increase in oxidative stress and mitochondrial dysfunction. This study elucidates the role of guanine-rich RNA sequence binding factor 1 (GRSF1) in the aging of ovarian granulosa cells (GCs). We observed a significant reduction in GRSF1 within GCs correlating with patient age, utilizing clinical samples from IVF patients. Using an siRNA-mediated knockdown technique, we established that diminished GRSF1 expression exacerbates mitochondrial dysfunction, elevates reactive oxygen species, and impairs ATP production. Furthermore, RNA immunoprecipitation revealed GRSF1's interaction with superoxide dismutase 2 (SOD2) mRNA, a key antioxidant enzyme, suggesting a mechanism whereby GRSF1 modulates oxidative stress. Downregulation of SOD2 reversed the protective effects of GRSF1 overexpression on mitochondrial function. These insights into the role of GRSF1 in ovarian aging may guide the development of interventions to improve fertility outcomes in advanced age. |
| المشرفين على المادة: | 0 (Reactive Oxygen Species) 0 (GRSF1 protein, human) EC 1.15.1.1 (superoxide dismutase 2) EC 1.15.1.1 (Superoxide Dismutase) 0 (Poly(A)-Binding Proteins) |
| تواريخ الأحداث: | Date Created: 20240531 Date Completed: 20240621 Latest Revision: 20240630 |
| رمز التحديث: | 20240630 |
| DOI: | 10.1530/REP-24-0015 |
| PMID: | 38819377 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1741-7899 |
|---|---|
| DOI: | 10.1530/REP-24-0015 |