دورية أكاديمية
Bacterial vampirism mediated through taxis to serum.
| العنوان: | Bacterial vampirism mediated through taxis to serum. |
|---|---|
| المؤلفون: | Glenn SJ; Washington State University, Department of Veterinary Microbiology and Pathology, Pullman, United States., Gentry-Lear Z; University of Oregon, Institute of Molecular Biology, Eugene, United States., Shavlik M; University of Oregon, Institute of Molecular Biology, Eugene, United States., Harms MJ; University of Oregon, Institute of Molecular Biology, Eugene, United States.; University of Oregon, Department of Chemistry & Biochemistry, Eugene, United States., Asaki TJ; Washington State University, Department of Mathematics and Statistics, Pullman, United States., Baylink A; Washington State University, Department of Veterinary Microbiology and Pathology, Pullman, United States. |
| المصدر: | ELife [Elife] 2024 May 31; Vol. 12. Date of Electronic Publication: 2024 May 31. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, UK : eLife Sciences Publications, Ltd., 2012- |
| مواضيع طبية MeSH: | Chemotaxis* , Serum*, Humans ; Serine/metabolism ; Enterobacteriaceae ; Animals ; Mice ; Salmonella enterica ; Bacterial Proteins/metabolism ; Bacterial Proteins/genetics |
| مستخلص: | Bacteria of the family Enterobacteriaceae are associated with gastrointestinal (GI) bleeding and bacteremia and are a leading cause of death, from sepsis, for individuals with inflammatory bowel diseases. The bacterial behaviors and mechanisms underlying why these bacteria are prone to bloodstream entry remain poorly understood. Herein, we report that clinical isolates of non-typhoidal Salmonella enterica serovars, Escherichia coli , and Citrobacter koseri are rapidly attracted toward sources of human serum. To simulate GI bleeding, we utilized an injection-based microfluidics device and found that femtoliter volumes of human serum are sufficient to induce bacterial attraction to the serum source. This response is orchestrated through chemotaxis and the chemoattractant L-serine, an amino acid abundant in serum that is recognized through direct binding by the chemoreceptor Tsr. We report the first crystal structures of Salmonella Typhimurium Tsr in complex with L-serine and identify a conserved amino acid recognition motif for L-serine shared among Tsr orthologues. We find Tsr to be widely conserved among Enterobacteriaceae and numerous World Health Organization priority pathogens associated with bloodstream infections. Lastly, we find that Enterobacteriaceae use human serum as a source of nutrients for growth and that chemotaxis and the chemoreceptor Tsr provide a competitive advantage for migration into enterohemorrhagic lesions. We define this bacterial behavior of taxis toward serum, colonization of hemorrhagic lesions, and the consumption of serum nutrients as 'bacterial vampirism', which may relate to the proclivity of Enterobacteriaceae for bloodstream infections. Competing Interests: SG, ZG, MS, MH, TA No competing interests declared, AB owns Amethyst Antimicrobials, LLC (© 2023, Glenn, Gentry-Lear et al.) |
| التعليقات: | Update of: bioRxiv. 2024 Jan 24:2023.07.07.548164. doi: 10.1101/2023.07.07.548164. (PMID: 37461633) |
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| معلومات مُعتمدة: | 1K99AI148587 National Institute of Allergy and Infectious Diseases; P30 GM124169 United States GM NIGMS NIH HHS; R00 AI148587 United States AI NIAID NIH HHS; 4R00AI148587-03 National Institute of Allergy and Infectious Diseases; K99 AI148587 United States AI NIAID NIH HHS; Startup fund Washington State University |
| فهرسة مساهمة: | Keywords: E. coli; Salmonella; Tsr; chemoreceptor; chemotaxis; infectious disease; microbiology; molecular biophysics; salmonella enterica typhimurium; serine; serum; structural biology Local Abstract: [plain-language-summary] Sepsis is the leading cause of death in patients with inflammatory bowel disease. Individuals with this condition can experience recurrent episodes of intestinal bleeding, giving intestinal (or enteric) bacteria an entry point into the bloodstream. This puts patients at risk of developing fatal infections – particularly from infections caused by bacteria belonging to the Enterobacteriaceae family. However, it is not well understood why this family of bacteria are particularly prone to entering the bloodstream. Enteric bacteria commonly respond to chemicals (or chemical stimuli) in their environment. This process, known as chemotaxis, helps bacteria with a variety of tasks, such as monitoring their environment, moving to different areas within their environment or colonizing their host. Chemical stimuli are classed as ‘attractants’ or ‘repellents’, with attractants luring the bacteria to an area and repellents discouraging the bacteria from being in a specific place. Intestinal bleeds will release serum (the liquid part of blood) into the gut, which could serve as a source of chemical stimuli to attract Enterobacteriaceae into the bloodstream. To find out more, Glen, Gentry-Lear et al. first used a microfluidic device to simulate an intestinal bleed and tested the response of Enterobacteriaceae bacteria to serum. Using chemotaxis, bacteria were found to be attracted to the amino acid L-serine in the serum to which they were able to attach through a receptor called Tsr. They also consumed nutrients present in the human serum to help them grow. Experiments with intestinal tissue showed that chemotaxis attracted bacteria to bleeding blood vessels and the Tsr receptor helped them to infiltrate the blood vessels. Glen et al. termed this attraction to and feeding upon blood serum as ‘bacterial vampirism’. These findings suggest that chemotaxis of Enterobacteriaceae towards L-serine in serum may be linked to their tendency to enter the bloodstream. Developing therapies that target chemotaxis in Enterobacteriaceae may provide a method for managing bloodstream infections. |
| المشرفين على المادة: | 452VLY9402 (Serine) 0 (Bacterial Proteins) |
| تواريخ الأحداث: | Date Created: 20240531 Date Completed: 20240531 Latest Revision: 20240612 |
| رمز التحديث: | 20240612 |
| مُعرف محوري في PubMed: | PMC11142651 |
| DOI: | 10.7554/eLife.93178 |
| PMID: | 38820052 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2050-084X |
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| DOI: | 10.7554/eLife.93178 |