دورية أكاديمية
From land to water: "Sunken" T-maze for associated learning in cichlid fish.
| العنوان: | From land to water: "Sunken" T-maze for associated learning in cichlid fish. |
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| المؤلفون: | Gedzun VR; Lactocore Inc, Newton, MA 02460, USA. Electronic address: gedzun@lactocore.com., Sukhanova IA; Lactocore Inc, Newton, MA 02460, USA., Aliper GM; Lactocore Inc, Newton, MA 02460, USA., Kotova MM; Lactocore Inc, Newton, MA 02460, USA., Melnik NO; Lactocore Inc, Newton, MA 02460, USA., Karimova EB; Lactocore Inc, Newton, MA 02460, USA., Voronkova AS; Lactocore Inc, Newton, MA 02460, USA., Coffman A; Lactocore Inc, Newton, MA 02460, USA., Pavshintcev VV; Lactocore Inc, Newton, MA 02460, USA., Mitkin NA; Lactocore Inc, Newton, MA 02460, USA., Doronin II; Lactocore Inc, Newton, MA 02460, USA., Babkin GA; Lactocore Inc, Newton, MA 02460, USA., Malyshev AV; Lactocore Inc, Newton, MA 02460, USA. |
| المصدر: | Behavioural brain research [Behav Brain Res] 2024 Aug 05; Vol. 471, pp. 115077. Date of Electronic Publication: 2024 May 31. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8004872 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7549 (Electronic) Linking ISSN: 01664328 NLM ISO Abbreviation: Behav Brain Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press. |
| مواضيع طبية MeSH: | Cichlids*/physiology , Conditioning, Operant*/drug effects , Conditioning, Operant*/physiology , Maze Learning*/drug effects , Maze Learning*/physiology , Dizocilpine Maleate*/pharmacology, Animals ; Male ; Female ; Reward ; Association Learning/physiology ; Association Learning/drug effects ; Excitatory Amino Acid Antagonists/pharmacology ; Excitatory Amino Acid Antagonists/administration & dosage ; Cues |
| مستخلص: | The study introduced and evaluated learning paradigms for Maylandia callainos cichlids using a modified version of the rodent T-maze, filled with tank water (the "sunken" modification). Both male and female fish underwent training in two distinct conditioning paradigms. Firstly, simple operant conditioning involved placing a food reward in either the right or left compartment. Cichlids demonstrated the ability to purposefully find the bait within 6 days of training, with a persistent place preference lasting up to 6 days. Additionally, the learning dynamics varied with sex: female cichlids exhibited reduction in latency to visit the target compartment and consume the bait, along with a decrease in the number of errors 3 and 4 days earlier than males, respectively. Secondly, visually-cued operant conditioning was conducted, with a food reward exclusively placed in the yellow compartment, randomly positioned on the left or right side of the maze during each training session. Visual learning persisted for 10 days until reaction time improvement plateaued. Color preference disappeared after 4 consecutive check-ups, with no sex-related interference. For further validation of visually-cued operant conditioning paradigm, drugs MK-801 (dizocilpine) and caffeine, known to affect performance in learning tasks, were administered intraperitoneally. Chronic MK-801 (0.17 mg/kg) impaired maze learning, resulting in no color preference development. Conversely, caffeine administration enhanced test performance, increasing precision in fish. This developed paradigm offers a viable approach for studying learning and memory and presents an effective alternative to rodent-based drug screening tools, exhibiting good face and predictive validity. Competing Interests: Declaration of Competing Interest At the time of completing the experiments and writing the manuscript, the team of authors was employed by Lactocore, Inc. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Animal model; Behavioral test; Drug screening; Experimental fish; Pharmacological intervention; Spatial associative learning |
| المشرفين على المادة: | 6LR8C1B66Q (Dizocilpine Maleate) 0 (Excitatory Amino Acid Antagonists) |
| تواريخ الأحداث: | Date Created: 20240602 Date Completed: 20240801 Latest Revision: 20240801 |
| رمز التحديث: | 20240802 |
| DOI: | 10.1016/j.bbr.2024.115077 |
| PMID: | 38825022 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7549 |
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| DOI: | 10.1016/j.bbr.2024.115077 |