دورية أكاديمية
The essential role of aggregation for the emulsifying ability of a fungal CYS-rich protein.
| العنوان: | The essential role of aggregation for the emulsifying ability of a fungal CYS-rich protein. |
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| المؤلفون: | Pitocchi R; Department of Chemical Sciences, University of Naples Federico II, Via Cintia, Naples, 80126, Italy., Cicatiello P; Department of Chemical Sciences, University of Naples Federico II, Via Cintia, Naples, 80126, Italy. paola.cicatiello@unina.it., Illiano A; Department of Chemical Sciences, University of Naples Federico II, Via Cintia, Naples, 80126, Italy., Fontanarosa C; Department of Chemical Sciences, University of Naples Federico II, Via Cintia, Naples, 80126, Italy., Spina F; Department of Life Sciences and Systems Biology, University of Turin, Viale P.A. Mattioli 25, Turin, 10125, Italy., Varese GC; Department of Life Sciences and Systems Biology, University of Turin, Viale P.A. Mattioli 25, Turin, 10125, Italy., Amoresano A; Department of Chemical Sciences, University of Naples Federico II, Via Cintia, Naples, 80126, Italy., Piscitelli A; Department of Chemical Sciences, University of Naples Federico II, Via Cintia, Naples, 80126, Italy., Giardina P; Department of Chemical Sciences, University of Naples Federico II, Via Cintia, Naples, 80126, Italy. |
| المصدر: | Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2024 Jun 03; Vol. 108 (1), pp. 358. Date of Electronic Publication: 2024 Jun 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin ; New York : Springer International, c1984- |
| مواضيع طبية MeSH: | Fusarium*/metabolism , Fusarium*/genetics , Fungal Proteins*/metabolism , Fungal Proteins*/chemistry , Fungal Proteins*/genetics , Surface-Active Agents*/metabolism , Surface-Active Agents*/chemistry, Emulsifying Agents/metabolism ; Emulsifying Agents/chemistry ; Soil Microbiology ; Emulsions/chemistry ; Emulsions/metabolism ; Surface Tension ; Cysteine/metabolism ; Cysteine/chemistry ; Olive Oil/metabolism ; Olive Oil/chemistry ; Mycelium/metabolism |
| مستخلص: | Biosurfactants are in demand by the global market as natural commodities suitable for incorporation into commercial products or utilization in environmental applications. Fungi are promising producers of these molecules and have garnered interest also for their metabolic capabilities in efficiently utilizing recalcitrant and complex substrates, like hydrocarbons, plastic, etc. Within this framework, biosurfactants produced by two Fusarium solani fungal strains, isolated from plastic waste-contaminated landfill soils, were analyzed. Mycelia of these fungi were grown in the presence of 5% olive oil to drive biosurfactant production. The characterization of the emulsifying and surfactant capacity of these extracts highlighted that two different components are involved. A protein was purified and identified as a CFEM (common in fungal extracellular membrane) containing domain, revealing a good propensity to stabilize emulsions only in its aggregate form. On the other hand, an unidentified cationic smaller molecule exhibits the ability to reduce surface tension. Based on the 3D structural model of the protein, a plausible mechanism for the formation of very stable aggregates, endowed with the emulsifying ability, is proposed. KEY POINTS: • Two Fusarium solani strains are analyzed for their surfactant production. • A cationic surfactant is produced, exhibiting the ability to remarkably reduce surface tension. • An identified protein reveals a good propensity to stabilize emulsions only in its aggregate form. (© 2024. The Author(s).) |
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| معلومات مُعتمدة: | PE00000004 European Union Next-Generation EU |
| فهرسة مساهمة: | Keywords: Aggregation; Bioemulsifier; CFEM domain; Fungal proteins; Plastic-degrading fungi |
| المشرفين على المادة: | 0 (Fungal Proteins) 0 (Surface-Active Agents) 0 (Emulsifying Agents) 0 (Emulsions) K848JZ4886 (Cysteine) 0 (Olive Oil) |
| تواريخ الأحداث: | Date Created: 20240603 Date Completed: 20240603 Latest Revision: 20240611 |
| رمز التحديث: | 20240611 |
| مُعرف محوري في PubMed: | PMC11147851 |
| DOI: | 10.1007/s00253-024-13182-7 |
| PMID: | 38829381 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1432-0614 |
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| DOI: | 10.1007/s00253-024-13182-7 |