دورية أكاديمية
Klenkia sesuvii sp. nov., isolated from leaves of halophyte Sesuvium portulacastrum .
| العنوان: | Klenkia sesuvii sp. nov., isolated from leaves of halophyte Sesuvium portulacastrum . |
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| المؤلفون: | Chantavorakit T; Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand., Thanompreechachai J; Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand., Suriyachadkun C; Thailand Bioresource Research Center (TBRC), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang, Pathum Thani 12120, Thailand., Duangmal K; Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.; Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, Thailand. |
| المصدر: | International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2024 Jun; Vol. 74 (6). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 100899600 Publication Model: Print Cited Medium: Internet ISSN: 1466-5034 (Electronic) Linking ISSN: 14665026 NLM ISO Abbreviation: Int J Syst Evol Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: Reading, UK : Society for General Microbiology, c2000- |
| مواضيع طبية MeSH: | Phylogeny* , Base Composition* , RNA, Ribosomal, 16S*/genetics , Fatty Acids*/chemistry , Plant Leaves*/microbiology , Salt-Tolerant Plants*/microbiology , DNA, Bacterial*/genetics , Bacterial Typing Techniques* , Sequence Analysis, DNA*, Thailand ; Vitamin K 2/analogs & derivatives ; Vitamin K 2/analysis ; Phospholipids/analysis ; Whole Genome Sequencing ; Genome, Bacterial |
| مستخلص: | During a study on the diversity of culturable actinobacteria from coastal halophytes in Thailand, strain LSe6-5 T was isolated from leaves of sea purslane ( Sesuvium portulacastrum L.), and a polyphasic approach was employed to determine its taxonomic position. The 16S rRNA gene sequences analysis indicated that the strain was most closely related to Klenkia brasiliensis Tu 6233 T (99.2 %), Klenkia marina YIM M13156 T (99.1 %), and Klenkia terrae PB261 T (98.7 %). The genome of strain LSe6-5 T was estimated to be 4.33 Mbp in size, with DNA G+C contents of 74.3%. A phylogenomic tree based on whole-genome sequences revealed that strain LSe6-5 T formed a clade with Klenkia marina DSM 45722 T , indicating their close relationship. However, the average nucleotide identity (ANI)-blast, ANI-MUMmer, and dDDH values between strain LSe6-5 T with K. marina DSM 45722 T (87.1, 88.9, and 33.0 %) were below the thresholds of 95-96 % ANI and 70 % dDDH for identifying a novel species. Furthermore, strain LSe6-5 T showed morphological and chemotaxonomic characteristics of the genus Klenkia . Cells were motile, rod-shaped, and Gram-stain-positive. Optimal growth of strain LSe6-5 T occurred at 28 °C, pH 7.0, and 0-3 % NaCl. The whole-cell hydrolysates contained meso -diaminopimelic acid as the diagnostic diamino acid, with galactose, glucose, mannose, and ribose as whole-cell sugars. The predominant menaquinones were MK-9(H |
| فهرسة مساهمة: | Keywords: actinomycete; plant leaves; polyphasic taxonomy; sea purslane |
| المشرفين على المادة: | 0 (RNA, Ribosomal, 16S) 0 (Fatty Acids) 0 (DNA, Bacterial) 11032-49-8 (Vitamin K 2) 0 (Phospholipids) |
| تواريخ الأحداث: | Date Created: 20240604 Date Completed: 20240604 Latest Revision: 20240604 |
| رمز التحديث: | 20240604 |
| DOI: | 10.1099/ijsem.0.006410 |
| PMID: | 38832855 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1466-5034 |
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| DOI: | 10.1099/ijsem.0.006410 |