دورية أكاديمية
The effect of daily usage of Listerine Cool Mint mouthwash on the oropharyngeal microbiome: a substudy of the PReGo trial.
| العنوان: | The effect of daily usage of Listerine Cool Mint mouthwash on the oropharyngeal microbiome: a substudy of the PReGo trial. |
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| المؤلفون: | Laumen JGE; Department of Clinical Sciences, Institute of Tropical Medicine, STI Unit, Antwerp, Belgium.; Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium., Van Dijck C; Department of Clinical Sciences, Institute of Tropical Medicine, STI Unit, Antwerp, Belgium.; Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium., Manoharan-Basil SS; Department of Clinical Sciences, Institute of Tropical Medicine, STI Unit, Antwerp, Belgium., de Block T; Department of Clinical Sciences, Clinical Reference Laboratory, Institute of Tropical Medicine, Antwerp, Belgium., Abdellati S; Department of Clinical Sciences, Clinical Reference Laboratory, Institute of Tropical Medicine, Antwerp, Belgium., Xavier BB; Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium., Malhotra-Kumar S; Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium., Kenyon C; Department of Clinical Sciences, Institute of Tropical Medicine, STI Unit, Antwerp, Belgium.; Department of Medicine, University of Cape Town, Cape Town, South Africa. |
| المصدر: | Journal of medical microbiology [J Med Microbiol] 2024 Jun; Vol. 73 (6). |
| نوع المنشور: | Journal Article; Randomized Controlled Trial |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 0224131 Publication Model: Print Cited Medium: Internet ISSN: 1473-5644 (Electronic) Linking ISSN: 00222615 NLM ISO Abbreviation: J Med Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: 1968-1995: Edinburgh, Livingstone. |
| مواضيع طبية MeSH: | Mouthwashes*/administration & dosage , Mouthwashes*/pharmacology , Salicylates*/pharmacology , Salicylates*/therapeutic use , Salicylates*/administration & dosage , Microbiota*/drug effects , Cross-Over Studies* , Oropharynx*/microbiology , Terpenes*/administration & dosage , Terpenes*/pharmacology, Humans ; Male ; Double-Blind Method ; Adult ; Drug Combinations ; Homosexuality, Male ; Gonorrhea/microbiology ; Gonorrhea/prevention & control ; HIV Infections/prevention & control ; Pre-Exposure Prophylaxis/methods ; Syphilis/prevention & control ; Syphilis/microbiology ; Bacteria/classification ; Bacteria/drug effects ; Bacteria/genetics ; Bacteria/isolation & purification |
| مستخلص: | Introduction. Listerine Ò is a bactericidal mouthwash widely used to prevent oral health problems such as dental plaque and gingivitis. However, whether it promotes or undermines a healthy oral microbiome is unclear. Hypothesis/Gap Statement. We hypothesized that the daily use of Listerine Cool Mint would have a significant impact on the oropharyngeal microbiome. Aim. We aimed to assess if daily usage of Listerine Cool Mint influenced the composition of the pharyngeal microbiome. Methodology. The current microbiome substudy is part of the Preventing Resistance in Gonorrhoea trial. This was a double-blind single-centre, crossover, randomized controlled trial of antibacterial versus placebo mouthwash to reduce the incidence of gonorrhoea/chlamydia/syphilis in men who have sex with men (MSM) taking HIV pre-exposure prophylaxis (PrEP). Fifty-nine MSM taking HIV PrEP were enrolled. In this crossover trial, participants received 3 months of daily Listerine followed by 3 months of placebo mouthwash or vice versa. Oropharyngeal swabs were taken at baseline and after 3 months use of each mouthwash. DNA was extracted for shotgun metagenomic sequencing (Illumina Inc.). Non-host reads were taxonomically classified with MiniKraken and Bracken. The alpha and beta diversity indices were compared between baseline and after each mouthwash use. Differentially abundant bacterial taxa were identified using ANOVA-like differential expression analysis. Results. Streptococcus was the most abundant genus in most samples ( n = 103, 61.7 %) with a median relative abundance of 31.5% (IQR 20.6-44.8), followed by Prevotella [13.5% (IQR 4.8-22.6)] and Veillonella [10.0% (IQR 4.0-16.8)]. Compared to baseline, the composition of the oral microbiome at the genus level (beta diversity) was significantly different after 3 months of Listerine ( P = 0.006, pseudo- F = 2.29) or placebo ( P = 0.003, pseudo- F = 2.49, permutational multivariate analysis of variance) use. Fusobacterium nucleatum and Streptococcus anginosus were significantly more abundant after Listerine use compared to baseline. Conclusion. Listerine use was associated with an increased abundance of common oral opportunistic bacteria previously reported to be enriched in periodontal diseases, oesophageal and colorectal cancer, and systemic diseases. These findings suggest that the regular use of Listerine mouthwash should be carefully considered. |
| فهرسة مساهمة: | Keywords: Listerine; antimicrobial resistance; microbiome; mouthwash |
| المشرفين على المادة: | 0 (Mouthwashes) 51273-66-6 (Listerine) 0 (Salicylates) 0 (Terpenes) 0 (Drug Combinations) |
| تواريخ الأحداث: | Date Created: 20240604 Date Completed: 20240604 Latest Revision: 20240717 |
| رمز التحديث: | 20240718 |
| DOI: | 10.1099/jmm.0.001830 |
| PMID: | 38833520 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1473-5644 |
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| DOI: | 10.1099/jmm.0.001830 |