دورية أكاديمية
LINC00520 promotes colorectal cancer progression through miRNA-195-3p / NAT2 axis.
| العنوان: | LINC00520 promotes colorectal cancer progression through miRNA-195-3p / NAT2 axis. |
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| المؤلفون: | Liu H; Department of Gastrointestinal Surgery, China-Japan Friendship Hospital, Beijing, China. dr_haibinliu1971@163.com., Zhang G; Department of Gastrointestinal Surgery, China-Japan Friendship Hospital, Beijing, China. zhgcmd@163.com., Li C; Department of Gastrointestinal Surgery, China-Japan Friendship Hospital, Beijing, China. li-chao-feng@139.com., Song X; Department of Gastrointestinal Surgery, China-Japan Friendship Hospital, Beijing, China. songxinxin1971@163.com. |
| المصدر: | Cellular and molecular biology (Noisy-le-Grand, France) [Cell Mol Biol (Noisy-le-grand)] 2024 Jun 05; Vol. 70 (6), pp. 187-191. Date of Electronic Publication: 2024 Jun 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: C.M.B. Association Country of Publication: France NLM ID: 9216789 Publication Model: Electronic Cited Medium: Internet ISSN: 1165-158X (Electronic) Linking ISSN: 01455680 NLM ISO Abbreviation: Cell Mol Biol (Noisy-le-grand) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2001->: Noisy-Le-Grand, France : C.M.B. Association Original Publication: Noisy-le-Grand, France : R. Wegmann, 1992- |
| مواضيع طبية MeSH: | MicroRNAs*/genetics , MicroRNAs*/metabolism , Colorectal Neoplasms*/genetics , Colorectal Neoplasms*/pathology , Colorectal Neoplasms*/metabolism , Gene Expression Regulation, Neoplastic* , Disease Progression* , RNA, Long Noncoding*/genetics , RNA, Long Noncoding*/metabolism , Arylamine N-Acetyltransferase*/genetics , Arylamine N-Acetyltransferase*/metabolism , Cell Movement*/genetics, Humans ; Cell Line, Tumor ; Neoplasm Invasiveness/genetics ; Cell Proliferation/genetics |
| مستخلص: | In this study, we investigated the role of LINC00520 in colorectal cancer (CRC) progression. We analyzed LINC00520 expression in 15 pairs of CRC tissues and adjacent tissues using qRT-PCR, revealing significantly elevated levels in CRC tissues and cell lines. Lentivirus-mediated up/down-regulation of LINC00520 in CRC cell lines demonstrated that increased LINC00520 expression enhanced cell invasiveness, as confirmed by transwell and wound healing assays. Bioinformatics analysis identified a regulatory axis involving LINC00520, microRNA-195-3p, and NAT2. Luciferase assays confirmed direct binding between LINC00520 and microRNA-195-3p, as well as microRNA-195-3p and NAT2. Overexpression of NAT2 reversed the inhibitory effects on invasion and migration induced by LINC00520 silencing. This suggests that LINC00520, highly expressed in CRC tissues, may modulate tumor biological functions through the microRNA-195-3p/NAT2 axis. Our findings provide insights into the mechanism underlying CRC progression, highlighting the potential of LINC00520 as a therapeutic target. |
| المشرفين على المادة: | 0 (MicroRNAs) 0 (MIRN195 microRNA, human) 0 (RNA, Long Noncoding) EC 2.3.1.5 (Arylamine N-Acetyltransferase) |
| تواريخ الأحداث: | Date Created: 20240605 Date Completed: 20240605 Latest Revision: 20240605 |
| رمز التحديث: | 20240605 |
| DOI: | 10.14715/cmb/2024.70.6.28 |
| PMID: | 38836661 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1165-158X |
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| DOI: | 10.14715/cmb/2024.70.6.28 |