دورية أكاديمية
أعرض في EDS

Auxin-mediated regulation of susceptibility to toxic metabolites, c-di-GMP levels, and phage infection in the rhizobacterium Serratia plymuthica .

التفاصيل البيبلوغرافية
العنوان: Auxin-mediated regulation of susceptibility to toxic metabolites, c-di-GMP levels, and phage infection in the rhizobacterium Serratia plymuthica .
المؤلفون: Rico-Jiménez M; Department of Biotechnology and Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain., Udaondo Z; Department of Biotechnology and Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain.; Department of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, Spain., Krell T; Department of Biotechnology and Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain., Matilla MA; Department of Biotechnology and Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain.
المصدر: MSystems [mSystems] 2024 Jul 23; Vol. 9 (7), pp. e0016524. Date of Electronic Publication: 2024 Jun 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101680636 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-5077 (Electronic) Linking ISSN: 23795077 NLM ISO Abbreviation: mSystems Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : American Society for Microbiology, [2016]-
مواضيع طبية MeSH: Indoleacetic Acids*/metabolism , Serratia*/metabolism , Serratia*/genetics , Serratia*/drug effects , Serratia*/pathogenicity , Cyclic GMP*/metabolism , Cyclic GMP*/analogs & derivatives, Gene Expression Regulation, Bacterial/drug effects ; Bacteriophages ; Signal Transduction/drug effects ; Biofilms/drug effects ; Biofilms/growth & development ; Bacterial Proteins/metabolism ; Bacterial Proteins/genetics ; Plant Growth Regulators/metabolism ; Transcriptome/drug effects
مستخلص: The communication between plants and their microbiota is highly dynamic and involves a complex network of signal molecules. Among them, the auxin indole-3-acetic acid (IAA) is a critical phytohormone that not only regulates plant growth and development, but is emerging as an important inter- and intra-kingdom signal that modulates many bacterial processes that are important during interaction with their plant hosts. However, the corresponding signaling cascades remain largely unknown. Here, we advance our understanding of the largely unknown mechanisms by which IAA carries out its regulatory functions in plant-associated bacteria. We showed that IAA caused important changes in the global transcriptome of the rhizobacterium Serratia plymuthica and multidisciplinary approaches revealed that IAA sensing interferes with the signaling mediated by other pivotal plant-derived signals such as amino acids and 4-hydroxybenzoic acid. Exposure to IAA caused large alterations in the transcript levels of genes involved in amino acid metabolism, resulting in significant metabolic alterations. IAA treatment also increased resistance to toxic aromatic compounds through the induction of the AaeXAB pump, which also confers resistance to IAA. Furthermore, IAA promoted motility and severely inhibited biofilm formation; phenotypes that were associated with decreased c-di-GMP levels and capsule production. IAA increased capsule gene expression and enhanced bacterial sensitivity to a capsule-dependent phage. Additionally, IAA induced the expression of several genes involved in antibiotic resistance and led to changes in the susceptibility and responses to antibiotics with different mechanisms of action. Collectively, our study illustrates the complexity of IAA-mediated signaling in plant-associated bacteria.
Importance: Signal sensing plays an important role in bacterial adaptation to ecological niches and hosts. This communication appears to be particularly important in plant-associated bacteria since they possess a large number of signal transduction systems that respond to a wide diversity of chemical, physical, and biological stimuli. IAA is emerging as a key inter- and intra-kingdom signal molecule that regulates a variety of bacterial processes. However, despite the extensive knowledge of the IAA-mediated regulatory mechanisms in plants, IAA signaling in bacteria remains largely unknown. Here, we provide insight into the diversity of mechanisms by which IAA regulates primary and secondary metabolism, biofilm formation, motility, antibiotic susceptibility, and phage sensitivity in a biocontrol rhizobacterium. This work has important implications for our understanding of bacterial ecology in plant environments and for the biotechnological and clinical applications of IAA, as well as related molecules.
Competing Interests: The authors declare no conflict of interest.
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معلومات مُعتمدة: PID2020-112612GB-I00 Spanish Ministry for Science and Innovation; PID2019-103972GA-I00 Spanish Ministry for Science and Innovation; 2023AEP002 Consejo Superior de Investigaciones Científicas (CSIC); P18-FR-1621 Junta de Andalucia
فهرسة مساهمة: Keywords: Bacteria; antibiotic susceptibility; auxin; biofilm; c-di-GMP; indole-3-acetic acid; phage sensitivity; resistance to toxic metabolites; signaling; transcriptomics
المشرفين على المادة: 0 (Indoleacetic Acids)
61093-23-0 (bis(3',5')-cyclic diguanylic acid)
H2D2X058MU (Cyclic GMP)
6U1S09C61L (indoleacetic acid)
0 (Bacterial Proteins)
0 (Plant Growth Regulators)
تواريخ الأحداث: Date Created: 20240605 Date Completed: 20240723 Latest Revision: 20240725
رمز التحديث: 20240726
مُعرف محوري في PubMed: PMC11264596
DOI: 10.1128/msystems.00165-24
PMID: 38837409
قاعدة البيانات: MEDLINE
الوصف
تدمد:2379-5077
DOI:10.1128/msystems.00165-24