دورية أكاديمية
أعرض في EDS

Establishment of dual reverse transcriptase-polymerase chain reaction for detection system for Areca palm velarivirus 1.

التفاصيل البيبلوغرافية
العنوان: Establishment of dual reverse transcriptase-polymerase chain reaction for detection system for Areca palm velarivirus 1.
المؤلفون: Peng C; Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan, China.; College of Life Sciences, Chongqing Normal University, Chongqing, China., Fan B; Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan, China., Zhu H; Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan, China., Liu L; Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan, China., Zhao Z; College of Life Sciences, Chongqing Normal University, Chongqing, China., Huang L; Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan, China.
المصدر: PloS one [PLoS One] 2024 Jun 05; Vol. 19 (6), pp. e0303941. Date of Electronic Publication: 2024 Jun 05 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Reverse Transcriptase Polymerase Chain Reaction*/methods , Plant Diseases*/virology, Arecaceae/virology ; Sensitivity and Specificity ; DNA Primers/genetics ; RNA, Viral/genetics ; RNA, Viral/analysis
مستخلص: Areca palm velarivirus 1 (APV1) is one of the main pathogen causing yellow leaf disease, and leading to considerable losses in the Areca palm industry. The detection methods for APV1 are primarily based on phenotype determination and molecular techniques, such as polymerase chain reaction (PCR). However, a single PCR has limitations in accuracy and sensitivity. Therefore, in the present study, we established a dual RT-PCR APV1-detection system with enhanced accuracy and sensitivity using two pairs of specific primers, YLDV2-F/YLDV2-R and YLDV4-F/YLDV4-R. Moreover, two cDNA fragments covering different regions of the viral genome were simultaneously amplified, with PCR amplicon of 311 and 499 bp, respectively. The dual RT-PCR detection system successfully amplified the two target regions of the APV1, demonstrating high specificity and sensitivity and compensating for the limitations of single-primer detection methods. We tested 60 Areca palm samples from different geographical regions, highlighting its advantages in that the dual RT-PCR system efficiently and accurately detected APV1 in samples across diverse areas. The dual RT-PCR APV1 detection system provides a rapid, accurate, and sensitive method for detecting the virus and offers valuable technical support for research in preventing and managing yellow leaf diseases caused by APV1 in Areca palms. Moreover, the findings of this study can serve as a reference for establishing similar plants viral detection systems in the future.
Competing Interests: no competing interests exist.
(Copyright: © 2024 Peng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: 0 (DNA Primers)
0 (RNA, Viral)
تواريخ الأحداث: Date Created: 20240605 Date Completed: 20240605 Latest Revision: 20240607
رمز التحديث: 20240607
مُعرف محوري في PubMed: PMC11152278
DOI: 10.1371/journal.pone.0303941
PMID: 38838001
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0303941