دورية أكاديمية
Expression and field evaluation of new Mycobacterium bovis antigens.
| العنوان: | Expression and field evaluation of new Mycobacterium bovis antigens. |
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| المؤلفون: | Villafañe L; Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina; Instituto de Agrobiotecnología y Biología Molecular, (IABIMO) INTA-CONICET, Argentina., Rocha RV; Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina; Instituto de Agrobiotecnología y Biología Molecular, (IABIMO) INTA-CONICET, Argentina., Bigi MM; Universidad de Buenos Aires, Facultad de Medicina, Argentina., Klepp LI; Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina; Instituto de Agrobiotecnología y Biología Molecular, (IABIMO) INTA-CONICET, Argentina., Taboga OA; Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina; Instituto de Agrobiotecnología y Biología Molecular, (IABIMO) INTA-CONICET, Argentina., Forrellad MA; Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina; Instituto de Agrobiotecnología y Biología Molecular, (IABIMO) INTA-CONICET, Argentina., López MG; Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina; Instituto de Agrobiotecnología y Biología Molecular, (IABIMO) INTA-CONICET, Argentina., Bigi F; Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina; Instituto de Agrobiotecnología y Biología Molecular, (IABIMO) INTA-CONICET, Argentina. Electronic address: bigi.fabiana@inta.gob.ar. |
| المصدر: | Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2024 Jul; Vol. 273, pp. 110788. Date of Electronic Publication: 2024 May 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Scientific Country of Publication: Netherlands NLM ID: 8002006 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2534 (Electronic) Linking ISSN: 01652427 NLM ISO Abbreviation: Vet Immunol Immunopathol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam] Elsevier Scientific. |
| مواضيع طبية MeSH: | Mycobacterium bovis*/immunology , Antigens, Bacterial*/immunology , Tuberculosis, Bovine*/immunology, Animals ; Cattle ; Bacterial Proteins/immunology ; Bacterial Proteins/genetics ; Tuberculin Test/veterinary ; Recombinant Proteins/immunology ; Recombinant Proteins/genetics |
| مستخلص: | Bovine tuberculosis (bTB) represents a threat to livestock production. Mycobacterium bovis is the main causative agent of bTB and a pathogen capable of infecting wildlife and humans. Eradication programs based on surveillance in slaughterhouses with mandatory testing and culling of reactive cattle have failed to eradicate bTB in many regions worldwide. Therefore, developing effective tools to control this disease is crucial. Using a computational tool, we identified proteins in the M. bovis proteome that carry predictive binding peptides to BoLADRB3.2 and selected Mb0309, Mb1090, Mb1810 and Mb3810 from all the identified proteins. The expression of these proteins in a baculovirus-insect cell expression system was successful only for Mb0309 and Mb3810. In parallel, we expressed the ESAT-6 family proteins EsxG and EsxH in this system. Among the recombinant proteins, Mb0309 and EsxG exhibited moderate performance in distinguishing between cattle that test positive and negative to bTB using the official test, the intradermal tuberculin test (IDT), when used to stimulate interferon-gamma production in blood samples from cattle. However, when combined as a protein cocktail, Mb0309 and EsxG were reactive in 50 % of positive cattle. Further assessments in cattle that evade the IDT (false negative) and cattle infected with Mycobacterium avium paratuberculosis are necessary to determine the potential utility of this cocktail as an additional tool to assist the accurate diagnosis of bTB. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antigens; EsxG; IFN-γ assay; Mb0309; Mycobacterium bovis |
| المشرفين على المادة: | 0 (Antigens, Bacterial) 0 (Bacterial Proteins) 0 (Recombinant Proteins) |
| تواريخ الأحداث: | Date Created: 20240605 Date Completed: 20240614 Latest Revision: 20240614 |
| رمز التحديث: | 20240615 |
| DOI: | 10.1016/j.vetimm.2024.110788 |
| PMID: | 38838485 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2534 |
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| DOI: | 10.1016/j.vetimm.2024.110788 |