دورية أكاديمية
Co-expression analysis reveals distinct alliances around two carbon fixation pathways in hydrothermal vent symbionts.
| العنوان: | Co-expression analysis reveals distinct alliances around two carbon fixation pathways in hydrothermal vent symbionts. |
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| المؤلفون: | Mitchell JH; Harvard University, Cambridge, MA, USA. jessicamitchell@fas.harvard.edu., Freedman AH; Harvard University, Cambridge, MA, USA., Delaney JA; Harvard University, Cambridge, MA, USA., Girguis PR; Harvard University, Cambridge, MA, USA. pgirguis@oeb.harvard.edu. |
| المصدر: | Nature microbiology [Nat Microbiol] 2024 Jun; Vol. 9 (6), pp. 1526-1539. Date of Electronic Publication: 2024 Jun 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101674869 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2058-5276 (Electronic) Linking ISSN: 20585276 NLM ISO Abbreviation: Nat Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : Nature Publishing Group, [2016]- |
| مواضيع طبية MeSH: | Hydrothermal Vents*/microbiology , Carbon Cycle* , Symbiosis* , Polychaeta*/metabolism, Animals ; Oxidation-Reduction ; Citric Acid Cycle ; Sulfides/metabolism ; Gene Expression Regulation, Bacterial ; Hydrogenase/metabolism ; Hydrogenase/genetics ; Chemoautotrophic Growth ; Gene Expression Profiling ; Nitrates/metabolism ; Photosynthesis ; Bacteria/metabolism ; Bacteria/genetics |
| مستخلص: | Most autotrophic organisms possess a single carbon fixation pathway. The chemoautotrophic symbionts of the hydrothermal vent tubeworm Riftia pachyptila, however, possess two functional pathways: the Calvin-Benson-Bassham (CBB) and the reductive tricarboxylic acid (rTCA) cycles. How these two pathways are coordinated is unknown. Here we measured net carbon fixation rates, transcriptional/metabolic responses and transcriptional co-expression patterns of Riftia pachyptila endosymbionts by incubating tubeworms collected from the East Pacific Rise at environmental pressures, temperature and geochemistry. Results showed that rTCA and CBB transcriptional patterns varied in response to different geochemical regimes and that each pathway is allied to specific metabolic processes; the rTCA is allied to hydrogenases and dissimilatory nitrate reduction, whereas the CBB is allied to sulfide oxidation and assimilatory nitrate reduction, suggesting distinctive yet complementary roles in metabolic function. Furthermore, our network analysis implicates the rTCA and a group 1e hydrogenase as key players in the physiological response to limitation of sulfide and oxygen. Net carbon fixation rates were also exemplary, and accordingly, we propose that co-activity of CBB and rTCA may be an adaptation for maintaining high carbon fixation rates, conferring a fitness advantage in dynamic vent environments. (© 2024. The Author(s).) |
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| معلومات مُعتمدة: | 9208 Gordon and Betty Moore Foundation (Gordon E. and Betty I. Moore Foundation); 1940100 National Science Foundation (NSF); 80NSSC19K1427 NASA | NASA Astrobiology Institute (NAI) |
| المشرفين على المادة: | 0 (Sulfides) EC 1.12.7.2 (Hydrogenase) 0 (Nitrates) |
| تواريخ الأحداث: | Date Created: 20240605 Date Completed: 20240605 Latest Revision: 20240605 |
| رمز التحديث: | 20240606 |
| DOI: | 10.1038/s41564-024-01704-y |
| PMID: | 38839975 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2058-5276 |
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| DOI: | 10.1038/s41564-024-01704-y |