Protective Effect of Aloe vera (L.) on Diabetes-Induced Oxidative Stress Linked Spermiological Co-Morbidity in Human and Rat: An In-Vitro Analysis.

التفاصيل البيبلوغرافية
العنوان:	Protective Effect of Aloe vera (L.) on Diabetes-Induced Oxidative Stress Linked Spermiological Co-Morbidity in Human and Rat: An In-Vitro Analysis.
المؤلفون:	Pal D; Molecular Medicine, Nutrigenomics and Public Health Research Laboratory, Department of Bio-Medical Laboratory Science and Management, Vidyasagar University, Paschim Midnapore, Midnapore, West Bengal, India., Mitra D; Molecular Medicine, Nutrigenomics and Public Health Research Laboratory, Department of Bio-Medical Laboratory Science and Management, Vidyasagar University, Paschim Midnapore, Midnapore, West Bengal, India., Hazra S; Molecular Medicine, Nutrigenomics and Public Health Research Laboratory, Department of Bio-Medical Laboratory Science and Management, Vidyasagar University, Paschim Midnapore, Midnapore, West Bengal, India., Ghosh D; Molecular Medicine, Nutrigenomics and Public Health Research Laboratory, Department of Bio-Medical Laboratory Science and Management, Vidyasagar University, Paschim Midnapore, Midnapore, West Bengal, India. debidasghosh999@gmail.com.
المصدر:	Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2024 Sep; Vol. 31 (9), pp. 2685-2699. Date of Electronic Publication: 2024 Jun 06.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Springer Country of Publication: United States NLM ID: 101291249 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1933-7205 (Electronic) Linking ISSN: 19337191 NLM ISO Abbreviation: Reprod Sci Subsets: MEDLINE
أسماء مطبوعة:	Publication: 2020- : [New York] : Springer Original Publication: Thousand Oaks, Calif. : Sage
مواضيع طبية MeSH:	Oxidative Stress/drug effects , Spermatozoa/drug effects , Spermatozoa/metabolism , Rats, Wistar , Plant Extracts/pharmacology , Aloe/chemistry , Diabetes Mellitus, Experimental/metabolism , Sperm Motility/drug effects, Male ; Animals ; Humans ; Rats ; Apoptosis/drug effects ; Antioxidants/pharmacology ; Comorbidity ; DNA Fragmentation/drug effects ; Cell Survival/drug effects
مستخلص:	Diabetes linked reproductive complications are rising problems nowadays. The study focused on the protective efficacy of Aloe vera (L.) on sperm cell damage in an oxidative stress milieu encumbered by a chronic diabetes in human and streptozotocin treated Wistar rat (Rattus norvegicus). Spermatozoa from rat's epididymal washing, and human semen after 3-4 days of abstinence of mating or masturbation were collected from control and diabetes groups. Spermatozoa of human and rat were incubated for 1 or 2 h at 37 ⁰ C in an in-vitro medium separately and considered as normo-glycemic control and diabetes sub-groups. Dose of 1 or, 2 or, 4 mg/ml of Aloe vera (L.) hydro-ethanolic (40:60) extract (AVHE) was given to diabetes samples, considered as sub-sub-group for assessing its protective effect on spermiological and oxidative stress parameters. The motility, viability, plasma membrane integrity, nuclear chromatin decondensation for DNA fragmentation, acrosome cap status, and antioxidative status of human and rat spermatozoa were decreased whereas spermatozoal apoptosis was elevated significantly (p < 0.05), noted by TUNEL assay in diabetes samples compared to the duration-matched control group. Exposure of AVHE to diabetes samples resulted significant rectification (p < 0.05) in the said parameters than the unexposed diabetes group. In control group, AVHE exposure has significant protective effect from spermiological deterioration compared to unexposed control group. Identification of major phytomolecules in AVHE was done by LC-MS study. Diabetes-induced oxidative stress-mediated spermatozoal injuries can be protected by AVHE extract, raise the possibility for potentiating sperm of human for increasing the success rate of in-vitro fertilization-blastocyst implantation. (© 2024. The Author(s), under exclusive licence to Society for Reproductive Investigation.)
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معلومات مُعتمدة:	DST/INSPIRE Fellowship/2018/IF180939 Department of Science and Technology, Govt. of India
فهرسة مساهمة:	Keywords: Aloe vera (L.); In-vitro study; Diabetes; Oxidative stress; Spermiological disorders
المشرفين على المادة:	0 (Plant Extracts) 0 (Antioxidants)
تواريخ الأحداث:	Date Created: 20240606 Date Completed: 20240913 Latest Revision: 20240925
رمز التحديث:	20240925
DOI:	10.1007/s43032-024-01605-9
PMID:	38844725
قاعدة البيانات:	MEDLINE

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تدمد:	1933-7205
DOI:	10.1007/s43032-024-01605-9