دورية أكاديمية
Topographical and histological analysis of keratinized mucosal grafts removal techniques an ex-vivo study in porcine mandibles.
العنوان: | Topographical and histological analysis of keratinized mucosal grafts removal techniques an ex-vivo study in porcine mandibles. |
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المؤلفون: | Dos Santos KVR; School of Dentistry, Sao Paulo University, Av. Do Café-Subsetor Oeste-11, Ribeirão Preto, SP, 14040-904, Brazil. klebervinicius@usp.br.; School of Dentistry, Federal University of Goiás, Av. Universitária, s/n-Setor Leste Universitário, Goiânia, GO, 74605-020, Brazil. klebervinicius@usp.br., Leles JLR; School of Dentistry, Paulista University, Rodovia BR 153, Km 503, s/n Fazenda Botafogo, Goiânia, GO, 74215060, Brazil., Roriz VM; School of Dentistry, Federal University of Goiás, Av. Universitária, s/n-Setor Leste Universitário, Goiânia, GO, 74605-020, Brazil., Leles CR; School of Dentistry, Federal University of Goiás, Av. Universitária, s/n-Setor Leste Universitário, Goiânia, GO, 74605-020, Brazil. |
المصدر: | Scientific reports [Sci Rep] 2024 Jun 06; Vol. 14 (1), pp. 13066. Date of Electronic Publication: 2024 Jun 06. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
مواضيع طبية MeSH: | Mouth Mucosa*/transplantation , Mouth Mucosa*/cytology , Mandible*/surgery, Animals ; Swine ; Keratins/metabolism ; Microscopy, Electron, Scanning ; Tissue and Organ Harvesting/methods |
مستخلص: | The aim of this study was to assess the surface and tissue quality of keratinized mucosa grafts (KMG) obtained using the conventional scalpel and mucotome techniques. This was an experimental in vitro/ex vivo study involving six porcine hemi-mandibles. Specimens were harvested using both the mucotome and conventional scalpel techniques, with randomization determining the choice of technique for tissue removal. The specimens were prepared following predefined laboratory protocols and subsequently subjected to optical microscopy for evaluating epithelial and connective tissue and scanning electron microscopy for topographical and 3D profilometry analysis. Tissues harvested using the mucotome exhibited a linear base and uniform thickness, along with the presence of submucosa and fibrous connective tissue, all of which are ideal for graft success. Differences in the surface characteristics of specimens obtained through the two techniques were observed during a comparative analysis of images obtained through both microscopy types. KMG obtained using the mucotome technique displayed greater uniformity and reduced undesirable cell presence compared to the scalpel technique, thereby enhancing the likelihood of success in soft tissue graft surgical procedures. This study provides valuable insights to oral healthcare professionals and may contribute to future research aimed at achieving more successful surgeries, shorter postoperative recovery times, reduced discomfort, and an overall more positive patient experience. (© 2024. The Author(s).) |
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فهرسة مساهمة: | Keywords: Keratinized mucosa grafts; Mucotome; Scanning electron microscope; Swine jaw |
المشرفين على المادة: | 68238-35-7 (Keratins) |
تواريخ الأحداث: | Date Created: 20240606 Date Completed: 20240606 Latest Revision: 20240609 |
رمز التحديث: | 20240609 |
مُعرف محوري في PubMed: | PMC11156962 |
DOI: | 10.1038/s41598-024-58559-w |
PMID: | 38844764 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2045-2322 |
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DOI: | 10.1038/s41598-024-58559-w |