دورية أكاديمية

Topographical and histological analysis of keratinized mucosal grafts removal techniques an ex-vivo study in porcine mandibles.

التفاصيل البيبلوغرافية
العنوان: Topographical and histological analysis of keratinized mucosal grafts removal techniques an ex-vivo study in porcine mandibles.
المؤلفون: Dos Santos KVR; School of Dentistry, Sao Paulo University, Av. Do Café-Subsetor Oeste-11, Ribeirão Preto, SP, 14040-904, Brazil. klebervinicius@usp.br.; School of Dentistry, Federal University of Goiás, Av. Universitária, s/n-Setor Leste Universitário, Goiânia, GO, 74605-020, Brazil. klebervinicius@usp.br., Leles JLR; School of Dentistry, Paulista University, Rodovia BR 153, Km 503, s/n Fazenda Botafogo, Goiânia, GO, 74215060, Brazil., Roriz VM; School of Dentistry, Federal University of Goiás, Av. Universitária, s/n-Setor Leste Universitário, Goiânia, GO, 74605-020, Brazil., Leles CR; School of Dentistry, Federal University of Goiás, Av. Universitária, s/n-Setor Leste Universitário, Goiânia, GO, 74605-020, Brazil.
المصدر: Scientific reports [Sci Rep] 2024 Jun 06; Vol. 14 (1), pp. 13066. Date of Electronic Publication: 2024 Jun 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Mouth Mucosa*/transplantation , Mouth Mucosa*/cytology , Mandible*/surgery, Animals ; Swine ; Keratins/metabolism ; Microscopy, Electron, Scanning ; Tissue and Organ Harvesting/methods
مستخلص: The aim of this study was to assess the surface and tissue quality of keratinized mucosa grafts (KMG) obtained using the conventional scalpel and mucotome techniques. This was an experimental in vitro/ex vivo study involving six porcine hemi-mandibles. Specimens were harvested using both the mucotome and conventional scalpel techniques, with randomization determining the choice of technique for tissue removal. The specimens were prepared following predefined laboratory protocols and subsequently subjected to optical microscopy for evaluating epithelial and connective tissue and scanning electron microscopy for topographical and 3D profilometry analysis. Tissues harvested using the mucotome exhibited a linear base and uniform thickness, along with the presence of submucosa and fibrous connective tissue, all of which are ideal for graft success. Differences in the surface characteristics of specimens obtained through the two techniques were observed during a comparative analysis of images obtained through both microscopy types. KMG obtained using the mucotome technique displayed greater uniformity and reduced undesirable cell presence compared to the scalpel technique, thereby enhancing the likelihood of success in soft tissue graft surgical procedures. This study provides valuable insights to oral healthcare professionals and may contribute to future research aimed at achieving more successful surgeries, shorter postoperative recovery times, reduced discomfort, and an overall more positive patient experience.
(© 2024. The Author(s).)
References: Periodontics. 1968 Aug;6(4):152-60. (PMID: 5243142)
J Prosthet Dent. 2000 Apr;83(4):402-11. (PMID: 10756289)
Clin Oral Investig. 2017 Jan;21(1):53-70. (PMID: 27873018)
Compend Suppl. 1994;(18):S699-705; quiz S714-7. (PMID: 8039208)
J Clin Periodontol. 2014 Apr;41 Suppl 15:S44-62. (PMID: 24641000)
Int J Periodontics Restorative Dent. 1994 Apr;14(2):126-37. (PMID: 7928129)
J Clin Periodontol. 2014 Jul;41(7):708-16. (PMID: 24708394)
J Periodontol. 1969 Mar;40(3):162-6. (PMID: 5253598)
J Clin Periodontol. 1977 Aug;4(3):191-9. (PMID: 330573)
J Periodontol. 2009 Jan;80(1):41-7. (PMID: 19228088)
J Periodontol. 1981 Feb;52(2):74-80. (PMID: 6164778)
Periodontal Clin Investig. 1998 Spring;20(1):12-20. (PMID: 9663118)
J Periodontol. 2020 Jan;91(1):9-16. (PMID: 31461778)
Dent Mater. 1999 Mar;15(2):79-86. (PMID: 10551099)
J Periodontol. 1985 Dec;56(12):715-20. (PMID: 3866056)
Clin Oral Investig. 2018 Jan;22(1):443-448. (PMID: 28551728)
Dent Med Probl. 2018 Apr-Jun;55(2):153-159. (PMID: 30152618)
Clin Oral Implants Res. 2009 Sep;20 Suppl 4:146-65. (PMID: 19663961)
J Periodontol. 1974 Feb;45(2):78-83. (PMID: 4520963)
J Periodontal Implant Sci. 2010 Aug;40(4):194-200. (PMID: 20827329)
J Periodontol. 2006 Aug;77(8):1410-20. (PMID: 16881810)
J Periodontol. 2000 Aug;71(8):1297-305. (PMID: 10972645)
J Periodontol. 2008 Mar;79(3):425-30. (PMID: 18315424)
J Clin Periodontol. 2012 Jul;39(7):674-80. (PMID: 22587305)
J Periodontol. 2005 Feb;76(2):178-86. (PMID: 15974840)
Head Neck. 2012 Dec;34(12):1777-81. (PMID: 22318761)
J Contemp Dent Pract. 2009 Jan 01;10(1):58-65. (PMID: 19142257)
J Periodontol. 1992 May;63(5):477-86. (PMID: 1527693)
SSO Schweiz Monatsschr Zahnheilkd. 1977 Aug;87(8):656-66. (PMID: 272041)
Clin Oral Implants Res. 2018 Mar;29 Suppl 15:32-49. (PMID: 29498129)
Quintessence Int. 2020;51(5):374-387. (PMID: 32159530)
Biomed Mater. 2011 Jun;6(3):035001. (PMID: 21487176)
J Periodontol. 2000 Nov;71(11):1729-36. (PMID: 11128921)
BMC Oral Health. 2019 Jul 15;19(1):148. (PMID: 31307447)
Int J Periodontics Restorative Dent. 2008 Oct;28(5):497-507. (PMID: 18991001)
Clin Oral Implants Res. 2008 Oct;19(10):991-6. (PMID: 18828814)
J Clin Periodontol. 1975 Apr;2(2):98-104. (PMID: 1055730)
J Prosthet Dent. 2015 May;113(5):383-90. (PMID: 25681355)
Int J Oral Implantol. 1987;4(2):49-58. (PMID: 3269837)
J Clin Periodontol. 2010 Aug 1;37(8):728-38. (PMID: 20590963)
J Clin Periodontol. 1979 Jun;6(3):158-64. (PMID: 288747)
فهرسة مساهمة: Keywords: Keratinized mucosa grafts; Mucotome; Scanning electron microscope; Swine jaw
المشرفين على المادة: 68238-35-7 (Keratins)
تواريخ الأحداث: Date Created: 20240606 Date Completed: 20240606 Latest Revision: 20240609
رمز التحديث: 20240609
مُعرف محوري في PubMed: PMC11156962
DOI: 10.1038/s41598-024-58559-w
PMID: 38844764
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-58559-w