دورية أكاديمية
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Single-cell transcriptome atlases of soybean root and mature nodule reveal new regulatory programs that control the nodulation process.

التفاصيل البيبلوغرافية
العنوان: Single-cell transcriptome atlases of soybean root and mature nodule reveal new regulatory programs that control the nodulation process.
المؤلفون: Cervantes-Pérez SA; Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68503, USA., Zogli P; Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68503, USA., Amini S; Division of Plant Science and Technology, College of Agriculture, Food, and Natural Resources, University of Missouri-Columbia, Columbia, MO 65211, USA; Interdisciplinary Plant Group of Missouri-Columbia, Columbia, MO 65211, USA., Thibivilliers S; Division of Plant Science and Technology, College of Agriculture, Food, and Natural Resources, University of Missouri-Columbia, Columbia, MO 65211, USA; Interdisciplinary Plant Group of Missouri-Columbia, Columbia, MO 65211, USA., Tennant S; Division of Plant Science and Technology, College of Agriculture, Food, and Natural Resources, University of Missouri-Columbia, Columbia, MO 65211, USA; Interdisciplinary Plant Group of Missouri-Columbia, Columbia, MO 65211, USA., Hossain MS; Division of Plant Science and Technology, College of Agriculture, Food, and Natural Resources, University of Missouri-Columbia, Columbia, MO 65211, USA; Interdisciplinary Plant Group of Missouri-Columbia, Columbia, MO 65211, USA., Xu H; Division of Plant Science and Technology, College of Agriculture, Food, and Natural Resources, University of Missouri-Columbia, Columbia, MO 65211, USA; Interdisciplinary Plant Group of Missouri-Columbia, Columbia, MO 65211, USA., Meyer I; Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68503, USA., Nooka A; Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68503, USA., Ma P; School of Computing, University of Nebraska-Lincoln, Lincoln, NE 68503, USA., Yao Q; School of Computing, University of Nebraska-Lincoln, Lincoln, NE 68503, USA., Naldrett MJ; Proteomics and Metabolomics Facility, Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE 68588, USA., Farmer A; National Center for Genome Resources, Santa Fe, NM 87505, USA., Martin O; INRAE, Université Paris-Saclay, Institut des Sciences des Plantes de Paris Saclay, IPS2, Batiment 630 Plateau du Moulon, Rue Noetzlin, 91192 Gif sur Yvette Cedex, France., Bhattacharya S; Resolve Biosciences, Monheim am Rhein, Germany., Kläver J; Resolve Biosciences, Monheim am Rhein, Germany., Libault M; Division of Plant Science and Technology, College of Agriculture, Food, and Natural Resources, University of Missouri-Columbia, Columbia, MO 65211, USA; Interdisciplinary Plant Group of Missouri-Columbia, Columbia, MO 65211, USA. Electronic address: libaultm@missouri.edu.
المصدر: Plant communications [Plant Commun] 2024 Aug 12; Vol. 5 (8), pp. 100984. Date of Electronic Publication: 2024 Jun 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Published by the Plant Communications Shanghai Office in association with Cell Press, an imprint of Elsevier Inc Country of Publication: China NLM ID: 101769147 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2590-3462 (Electronic) Linking ISSN: 25903462 NLM ISO Abbreviation: Plant Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Shanghai] : Published by the Plant Communications Shanghai Office in association with Cell Press, an imprint of Elsevier Inc., [2020]-
مواضيع طبية MeSH: Glycine max*/genetics , Glycine max*/microbiology , Transcriptome* , Plant Root Nodulation*/genetics , Root Nodules, Plant*/genetics , Root Nodules, Plant*/microbiology , Root Nodules, Plant*/metabolism, Plant Roots/genetics ; Plant Roots/microbiology ; Single-Cell Analysis ; Gene Expression Regulation, Plant ; Symbiosis/genetics ; Nitrogen Fixation/genetics ; Bradyrhizobium/genetics ; Bradyrhizobium/physiology
مستخلص: The soybean root system is complex. In addition to being composed of various cell types, the soybean root system includes the primary root, the lateral roots, and the nodule, an organ in which mutualistic symbiosis with N-fixing rhizobia occurs. A mature soybean root nodule is characterized by a central infection zone where atmospheric nitrogen is fixed and assimilated by the symbiont, resulting from the close cooperation between the plant cell and the bacteria. To date, the transcriptome of individual cells isolated from developing soybean nodules has been established, but the transcriptomic signatures of cells from the mature soybean nodule have not yet been characterized. Using single-nucleus RNA-seq and Molecular Cartography technologies, we precisely characterized the transcriptomic signature of soybean root and mature nodule cell types and revealed the co-existence of different sub-populations of B. diazoefficiens-infected cells in the mature soybean nodule, including those actively involved in nitrogen fixation and those engaged in senescence. Mining of the single-cell-resolution nodule transcriptome atlas and the associated gene co-expression network confirmed the role of known nodulation-related genes and identified new genes that control the nodulation process. For instance, we functionally characterized the role of GmFWL3, a plasma membrane microdomain-associated protein that controls rhizobial infection. Our study reveals the unique cellular complexity of the mature soybean nodule and helps redefine the concept of cell types when considering the infection zone of the soybean nodule.
(Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: nanodomains; nodule; root; single-cell RNA-seq; soybean
تواريخ الأحداث: Date Created: 20240607 Date Completed: 20240813 Latest Revision: 20240813
رمز التحديث: 20240814
DOI: 10.1016/j.xplc.2024.100984
PMID: 38845198
قاعدة البيانات: MEDLINE
الوصف
تدمد:2590-3462
DOI:10.1016/j.xplc.2024.100984