دورية أكاديمية
The toxicity of superparamagnetic iron oxide nanoparticles induced on the testicular cells: In vitro study.
| العنوان: | The toxicity of superparamagnetic iron oxide nanoparticles induced on the testicular cells: In vitro study. |
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| المؤلفون: | Dantas GPF; Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Ferraz FS; Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Coimbra JLP; Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Paniago RM; Department of Physics, ICEx, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Dantas MSS; Metallurgical and Materials Engineering Department, EE, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Lacerda SMSN; Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Procópio MS; Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Gonçalves MF; Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Furtado MH; Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil; Clínica MF Fertilidade Masculina, Belo Horizonte, MG, Brazil., Mendes BP; Clínica MF Fertilidade Masculina, Belo Horizonte, MG, Brazil., López JL; Center for Biological and Natural Sciences, Federal University of Acre, Rio Branco, Acre, Brazil., Krohling AC; Centro de Desenvolvimento da Tecnologia Nuclear, CDTN, 31270-901, Belo Horizonte, MG, Brazil., Martins EMN; Centro de Desenvolvimento da Tecnologia Nuclear, CDTN, 31270-901, Belo Horizonte, MG, Brazil., Andrade LM; Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil; Department of Physics, ICEx, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Ladeira LO; Metallurgical and Materials Engineering Department, EE, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Andrade ÂL; Department of Chemistry, ICEB, Federal University of Ouro Preto, Ouro Preto, MG, Brazil., Costa GMJ; Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: gmjc@ufmg.br. |
| المصدر: | NanoImpact [NanoImpact] 2024 Jun 06; Vol. 35, pp. 100517. Date of Electronic Publication: 2024 Jun 06. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101676795 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2452-0748 (Electronic) Linking ISSN: 24520748 NLM ISO Abbreviation: NanoImpact Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam] : Elsevier B.V., [2016]- |
| مستخلص: | Superparamagnetic iron oxide nanoparticles (SPIONs) have gained significant attention in biomedical research due to their potential applications. However, little is known about their impact and toxicity on testicular cells. To address this issue, we conducted an in vitro study using primary mouse testicular cells, testis fragments, and sperm to investigate the cytotoxic effects of sodium citrate-coated SPIONs (Cit_SPIONs). Herein, we synthesized and physiochemically characterized the Cit_SPIONs and observed that the sodium citrate diminished the size and improved the stability of nanoparticles in solution during the experimental time. The sodium citrate (measured by thermogravimetry) was biocompatible with testicular cells at the used concentration (3%). Despite these favorable physicochemical properties, the in vitro experiments demonstrated the cytotoxicity of Cit_SPIONs, particularly towards testicular somatic cells and sperm cells. Transmission electron microscopy analysis confirmed that Leydig cells preferentially internalized Cit_SPIONs in the organotypic culture system, which resulted in alterations in their cytoplasmic size. Additionally, we found that Cit_SPIONs exposure had detrimental effects on various parameters of sperm cells, including motility, viability, DNA integrity, mitochondrial activity, lipid peroxidation (LPO), and ROS production. Our findings suggest that testicular somatic cells and sperm cells are highly sensitive and vulnerable to Cit_SPIONs and induced oxidative stress. This study emphasizes the potential toxicity of SPIONs, indicating significant threats to the male reproductive system. Our findings highlight the need for detailed development of iron oxide nanoparticles to enhance reproductive nanosafety. Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest regarding the publication of this manuscript. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Nanotoxicity; Oxidative stress; SPIONs; Testicular cells; Ultrastructural alterations |
| تواريخ الأحداث: | Date Created: 20240607 Latest Revision: 20240616 |
| رمز التحديث: | 20240617 |
| DOI: | 10.1016/j.impact.2024.100517 |
| PMID: | 38848992 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2452-0748 |
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| DOI: | 10.1016/j.impact.2024.100517 |