دورية أكاديمية
ASXLs binding to the PHD2/3 fingers of MLL4 provides a mechanism for the recruitment of BAP1 to active enhancers.
العنوان: | ASXLs binding to the PHD2/3 fingers of MLL4 provides a mechanism for the recruitment of BAP1 to active enhancers. |
---|---|
المؤلفون: | Zhang Y; Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO, 80045, USA.; Department of Biochemistry, Case Western Reserve University, Cleveland, OH, 44106, USA., Xie G; Laboratory of Endocrinology and Receptor Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, 20892, USA., Lee JE; Laboratory of Endocrinology and Receptor Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, 20892, USA., Zandian M; Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO, 80045, USA., Sudarshan D; St-Patrick Research Group in Basic Oncology, Oncology Division of CHU de Québec-Université Laval Research, Laval University Cancer Research Center, Quebec City, QC, G1R 3S3, Canada., Estavoyer B; Maisonneuve-Rosemont Hospital Research Center, Montréal, QC, H1T 2M4, Canada., Benz C; Department of Chemistry, BMC, Uppsala University, Uppsala, 75237, Sweden., Viita T; St-Patrick Research Group in Basic Oncology, Oncology Division of CHU de Québec-Université Laval Research, Laval University Cancer Research Center, Quebec City, QC, G1R 3S3, Canada., Asgaritarghi G; St-Patrick Research Group in Basic Oncology, Oncology Division of CHU de Québec-Université Laval Research, Laval University Cancer Research Center, Quebec City, QC, G1R 3S3, Canada., Lachance C; St-Patrick Research Group in Basic Oncology, Oncology Division of CHU de Québec-Université Laval Research, Laval University Cancer Research Center, Quebec City, QC, G1R 3S3, Canada., Messmer C; Maisonneuve-Rosemont Hospital Research Center, Montréal, QC, H1T 2M4, Canada., Simonetti L; Department of Chemistry, BMC, Uppsala University, Uppsala, 75237, Sweden., Sinha VK; Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO, 80045, USA., Lambert JP; St-Patrick Research Group in Basic Oncology, Oncology Division of CHU de Québec-Université Laval Research, Laval University Cancer Research Center, Quebec City, QC, G1R 3S3, Canada., Chen YW; Institute of Biomedical Sciences, Academia Sinica, Taipei, 11529, Taiwan, ROC., Wang SP; Institute of Biomedical Sciences, Academia Sinica, Taipei, 11529, Taiwan, ROC., Ivarsson Y; Department of Chemistry, BMC, Uppsala University, Uppsala, 75237, Sweden., Affar EB; Maisonneuve-Rosemont Hospital Research Center, Montréal, QC, H1T 2M4, Canada.; Department of Medicine, University of Montréal, Montréal, QC, H3C 3J7, Canada., Côté J; St-Patrick Research Group in Basic Oncology, Oncology Division of CHU de Québec-Université Laval Research, Laval University Cancer Research Center, Quebec City, QC, G1R 3S3, Canada. jacques.cote@crchudequebec.ulaval.ca., Ge K; Laboratory of Endocrinology and Receptor Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, 20892, USA. kai.ge@nih.gov., Kutateladze TG; Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO, 80045, USA. tatiana.kutateladze@cuanschutz.edu. |
المصدر: | Nature communications [Nat Commun] 2024 Jun 07; Vol. 15 (1), pp. 4883. Date of Electronic Publication: 2024 Jun 07. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [London] : Nature Pub. Group |
مواضيع طبية MeSH: | Tumor Suppressor Proteins*/metabolism , Tumor Suppressor Proteins*/genetics , Histone-Lysine N-Methyltransferase*/metabolism , Histone-Lysine N-Methyltransferase*/genetics , Ubiquitin Thiolesterase*/metabolism , Ubiquitin Thiolesterase*/genetics , Repressor Proteins*/metabolism , Repressor Proteins*/genetics , Protein Binding* , DNA-Binding Proteins*/metabolism , DNA-Binding Proteins*/genetics, Humans ; Animals ; Mice ; Enhancer Elements, Genetic ; HEK293 Cells ; PHD Zinc Fingers ; Histones/metabolism |
مستخلص: | The human methyltransferase and transcriptional coactivator MLL4 and its paralog MLL3 are frequently mutated in cancer. MLL4 and MLL3 monomethylate histone H3K4 and contain a set of uncharacterized PHD fingers. Here, we report a novel function of the PHD2 and PHD3 (PHD2/3) fingers of MLL4 and MLL3 that bind to ASXL2, a component of the Polycomb repressive H2AK119 deubiquitinase (PR-DUB) complex. The structure of MLL4 PHD2/3 in complex with the MLL-binding helix (MBH) of ASXL2 and mutational analyses reveal the molecular mechanism which is conserved in homologous ASXL1 and ASXL3. The native interaction of the Trithorax MLL3/4 complexes with the PR-DUB complex in vivo depends solely on MBH of ASXL1/2, coupling the two histone modifying activities. ChIP-seq analysis in embryonic stem cells demonstrates that MBH of ASXL1/2 is required for the deubiquitinase BAP1 recruitment to MLL4-bound active enhancers. Our findings suggest an ASXL1/2-dependent functional link between the MLL3/4 and PR-DUB complexes. (© 2024. The Author(s).) |
References: | Oncogene. 2017 Sep 28;36(39):5497-5511. (PMID: 28534508) Nat Rev Cancer. 2013 Mar;13(3):153-9. (PMID: 23550303) Biochem Soc Trans. 2021 Jun 30;49(3):1041-1054. (PMID: 34156443) Cell Rep. 2015 Oct 20;13(3):621-633. (PMID: 26456817) Gene. 2017 Sep 5;627:337-342. (PMID: 28669924) Mol Cell Biol. 2010 Nov;30(21):5071-85. (PMID: 20805357) J Biol Chem. 2011 Jan 14;286(2):1354-63. (PMID: 21047783) Nat Commun. 2016 Jan 07;7:10292. (PMID: 26739236) Nature. 2006 Jul 6;442(7098):91-5. (PMID: 16728978) Methods Mol Biol. 2020;2141:529-552. (PMID: 32696376) Nature. 2010 May 13;465(7295):243-7. (PMID: 20436459) Nature. 2006 Jul 6;442(7098):86-90. (PMID: 16728976) Mol Cell Biol. 2013 Dec;33(23):4745-54. (PMID: 24081332) Front Cell Dev Biol. 2021 Sep 16;9:729338. (PMID: 34604228) Nature. 2007 Aug 9;448(7154):714-7. (PMID: 17687327) Nucleic Acids Res. 2017 Jun 20;45(11):6388-6403. (PMID: 28398509) Nature. 2017 Aug 17;548(7667):297-303. (PMID: 28783718) Nat Commun. 2019 Jan 3;10(1):36. (PMID: 30604749) Nat Commun. 2019 May 24;10(1):2314. (PMID: 31127101) Proc Natl Acad Sci U S A. 2016 Oct 18;113(42):11871-11876. (PMID: 27698142) Nat Genet. 2012 Jan 08;44(2):148-56. (PMID: 22231485) Cell. 2009 Jul 23;138(2):389-403. (PMID: 19615732) Methods Enzymol. 2016;573:303-18. (PMID: 27372759) EMBO J. 2011 Jan 19;30(2):249-62. (PMID: 21131905) J Biol Chem. 2015 Nov 27;290(48):28643-63. (PMID: 26416890) Nature. 2006 Jul 6;442(7098):96-9. (PMID: 16728974) Nat Rev Cancer. 2015 Jun;15(6):334-46. (PMID: 25998713) Nat Genet. 2023 Apr;55(4):693-705. (PMID: 37012455) Mol Cell Proteomics. 2022 Jul;21(7):100253. (PMID: 35636729) Nature. 2006 Jul 6;442(7098):100-3. (PMID: 16728977) Mol Syst Biol. 2022 Jan;18(1):e10584. (PMID: 35044719) Cell Rep. 2013 Jul 11;4(1):148-58. (PMID: 23831028) Cancer Cell. 2012 Aug 14;22(2):180-93. (PMID: 22897849) Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286) Biochim Biophys Acta. 2014 Feb;1843(2):366-71. (PMID: 24291127) Nat Commun. 2018 Oct 22;9(1):4385. (PMID: 30349006) Nat Commun. 2019 Jan 21;10(1):348. (PMID: 30664650) Annu Rev Biochem. 2020 Jun 20;89:235-253. (PMID: 31928411) Nature. 2007 Aug 9;448(7154):718-22. (PMID: 17687328) Nat Biotechnol. 2011 Jan;29(1):24-6. (PMID: 21221095) Cell Death Differ. 2021 Feb;28(2):606-625. (PMID: 33462414) Nature. 2013 Oct 17;502(7471):333-339. (PMID: 24132290) Nat Med. 2018 Jun;24(6):758-769. (PMID: 29785026) Elife. 2013 Dec 24;2:e01503. (PMID: 24368734) Nat Commun. 2023 Feb 8;14(1):697. (PMID: 36754959) J Biol Chem. 2007 Jul 13;282(28):20395-406. (PMID: 17500065) |
معلومات مُعتمدة: | K99 CA241301 United States CA NCI NIH HHS; R00 CA241301 United States CA NCI NIH HHS; AG067664 U.S. Department of Health & Human Services | NIH | National Institute on Aging (U.S. National Institute on Aging) |
المشرفين على المادة: | 0 (Tumor Suppressor Proteins) EC 2.1.1.43 (Histone-Lysine N-Methyltransferase) EC 3.4.19.12 (Ubiquitin Thiolesterase) 0 (BAP1 protein, human) EC 2.1.1.43 (MLL4 protein, human) 0 (Repressor Proteins) 0 (DNA-Binding Proteins) 0 (KMT2C protein, human) 0 (ASXL2 protein, human) 0 (ASXL1 protein, human) 0 (Histones) |
تواريخ الأحداث: | Date Created: 20240607 Date Completed: 20240607 Latest Revision: 20240617 |
رمز التحديث: | 20240617 |
مُعرف محوري في PubMed: | PMC11161652 |
DOI: | 10.1038/s41467-024-49391-x |
PMID: | 38849395 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2041-1723 |
---|---|
DOI: | 10.1038/s41467-024-49391-x |