دورية أكاديمية
أعرض في EDS

Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes.

التفاصيل البيبلوغرافية
العنوان: Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes.
المؤلفون: Moldovan JB; Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA., Kopera HC; Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA., Liu Y; Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA., Garcia-Canadas M; Department of Genomic Medicine, GENYO, Centre for Genomics and Oncological Research, Pfizer-University of Granada-Andalusian Regional Government, PTS Granada 18016, Spain., Catalina P; Andalusian Public Health System Biobank, Granada 18016, Spain., Leone PE; Genetics and Genomics Laboratory, SOLCA Hospital, Quito, Ecuador., Sanchez L; Department of Genomic Medicine, GENYO, Centre for Genomics and Oncological Research, Pfizer-University of Granada-Andalusian Regional Government, PTS Granada 18016, Spain., Kitzman JO; Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA.; Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI 48109, USA., Kidd JM; Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA.; Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI 48109, USA., Garcia-Perez JL; Department of Genomic Medicine, GENYO, Centre for Genomics and Oncological Research, Pfizer-University of Granada-Andalusian Regional Government, PTS Granada 18016, Spain., Moran JV; Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA.; Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA.
المصدر: Nucleic acids research [Nucleic Acids Res] 2024 Jul 22; Vol. 52 (13), pp. 7761-7779.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
مواضيع طبية MeSH: Alu Elements*/genetics , Long Interspersed Nucleotide Elements*/genetics, Humans ; HeLa Cells ; Short Interspersed Nucleotide Elements/genetics ; Animals ; Retroelements/genetics ; RNA/genetics ; RNA/metabolism ; RNA, Transfer/genetics ; RNA, Transfer/metabolism ; Zebrafish/genetics
مستخلص: Alu elements are non-autonomous Short INterspersed Elements (SINEs) derived from the 7SL RNA gene that are present at over one million copies in human genomic DNA. Alu mobilizes by a mechanism known as retrotransposition, which requires the Long INterspersed Element-1 (LINE-1) ORF2-encoded protein (ORF2p). Here, we demonstrate that HeLa strains differ in their capacity to support Alu retrotransposition. Human Alu elements retrotranspose efficiently in HeLa-HA and HeLa-CCL2 (Alu-permissive) strains, but not in HeLa-JVM or HeLa-H1 (Alu-nonpermissive) strains. A similar pattern of retrotransposition was observed for other 7SL RNA-derived SINEs and tRNA-derived SINEs. In contrast, mammalian LINE-1s, a zebrafish LINE, a human SINE-VNTR-Alu (SVA) element, and an L1 ORF1-containing mRNA can retrotranspose in all four HeLa strains. Using an in vitro reverse transcriptase-based assay, we show that Alu RNAs associate with ORF2p and are converted into cDNAs in both Alu-permissive and Alu-nonpermissive HeLa strains, suggesting that 7SL- and tRNA-derived SINEs use strategies to 'hijack' L1 ORF2p that are distinct from those used by SVA elements and ORF1-containing mRNAs. These data further suggest ORF2p associates with the Alu RNA poly(A) tract in both Alu-permissive and Alu-nonpermissive HeLa strains, but that Alu retrotransposition is blocked after this critical step in Alu-nonpermissive HeLa strains.
(© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.)
التعليقات: Update of: bioRxiv. 2024 May 03:2024.05.03.592410. doi: 10.1101/2024.05.03.592410. (PMID: 38746229)
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معلومات مُعتمدة: R01 GM060518 United States GM NIGMS NIH HHS; University of Michigan Rogel Cancer Center First and Goal Award; PAIDI P12-CTS-2256 Andalusian regional Government; SAF2017-89745-R Government of Spain; R21 CA219300 United States CA NCI NIH HHS; R01 GM140135 United States GM NIGMS NIH HHS; Ms. Francisca Serrano; R21 CA219300 United States NH NIH HHS; R21 CA219300 United States NH NIH HHS; ERC-STG-2012-309433 International ERC_ European Research Council
المشرفين على المادة: 0 (Retroelements)
63231-63-0 (RNA)
9014-25-9 (RNA, Transfer)
تواريخ الأحداث: Date Created: 20240608 Date Completed: 20240721 Latest Revision: 20240723
رمز التحديث: 20240723
مُعرف محوري في PubMed: PMC11260458
DOI: 10.1093/nar/gkae448
PMID: 38850156
قاعدة البيانات: MEDLINE
الوصف
تدمد:1362-4962
DOI:10.1093/nar/gkae448