دورية أكاديمية
أعرض في EDS

Transcriptomic responses of peripheral blood leukocytes to cardiac surgery after acute inflammation, and three months recovery.

التفاصيل البيبلوغرافية
العنوان: Transcriptomic responses of peripheral blood leukocytes to cardiac surgery after acute inflammation, and three months recovery.
المؤلفون: Lewallen EA; Department of Biological Sciences, Hampton University, Hampton, VA, USA. Electronic address: eric.lewallen@hamptonu.edu., Liu D; Department of Obstetrics and Gynecology, Shengjin Hospital of China Medical University, Shenyang, Peoples Republic of China. Electronic address: lid1@sj-hospital.org., Karwoski J; Department of Undergraduate Studies, Drexel University, Philadelphia, PA, USA. Electronic address: jk3686@drexel.edu., Szeto WY; Division of Cardiovascular Surgery, University of Pennsylvania, Philadelphia, PA, USA. Electronic address: wilson.szeto@pennmedicine.upenn.edu., van Wijnen AJ; Department of Biochemistry, University of Vermont, Burlington, VT, USA. Electronic address: andre.vanwijnen@uvm.edu., Laudanski K; Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, Rochester, MN, USA. Electronic address: laudanski.krzysztof@mayo.edu.
المصدر: Genomics [Genomics] 2024 Jun 06; Vol. 116 (5), pp. 110878. Date of Electronic Publication: 2024 Jun 06.
Publication Model: Ahead of Print
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: United States NLM ID: 8800135 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1089-8646 (Electronic) Linking ISSN: 08887543 NLM ISO Abbreviation: Genomics Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Diego : Academic Press, [c1987-
مستخلص: Traumatic perioperative conditions may trigger early systemic responses, activate leukocytes and reprogram the immune system. We hypothesize that leukocyte activation may not revert to pre-surgical states, and that protracted activation may emerge with increased risks of comorbidities. We tested this concept by examining the transcriptomes of monocytes and T cells in a representative observational cohort of patients (n = 13) admitted for elective cardiac surgery. Transcriptomes in T cells and monocytes were compared from before surgery (t 0 ), and monocytes were analyzed longitudinally after acute (t 24hr ), and convalescent (t 3m ) time points. Monocytes and T cells expressed distinct transcriptomes, reflected by statistically significant differential expression of 558 T cell related genes. Monocytes expressed genes related to protein degradation and presented atypical activation of surface markers and cytoplasmic functions over time. Additionally, monocytes exhibited limited transcriptomic heterogeneity prior to surgery, and long-term patterns of gene expression associated with atherosclerosis showed three temporally distinct signatures. These data establish that post-cardiac surgery transcriptomes of monocytes differ even at three months compared to baselines, which may reflect latent ('smoldering') inflammation and persistent progression of tissue degenerative processes that should inform clinical care.
Competing Interests: Declaration of competing interest None of the authors has a conflict of interests.
(Copyright © 2024. Published by Elsevier Inc.)
فهرسة مساهمة: Keywords: Bioinformatics; Cardiology; Monocyte; RNA-seq; Trauma
تواريخ الأحداث: Date Created: 20240608 Latest Revision: 20240618
رمز التحديث: 20240619
DOI: 10.1016/j.ygeno.2024.110878
PMID: 38851465
قاعدة البيانات: MEDLINE
الوصف
تدمد:1089-8646
DOI:10.1016/j.ygeno.2024.110878