دورية أكاديمية
أعرض في EDS

Trypanosoma cruzi infection modulates secreted phospholipase A 2 expression in the salivary glands of Rhodnius prolixus.

التفاصيل البيبلوغرافية
العنوان: Trypanosoma cruzi infection modulates secreted phospholipase A 2 expression in the salivary glands of Rhodnius prolixus.
المؤلفون: de Araujo MFC; Laboratório de Bioquímica de Lipídios e Lipoproteínas, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Cardoso LS; Laboratório de Bioquímica de Lipídios e Lipoproteínas, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Pereira MH; Laboratório de Fisiologia de Insetos Hematófagos, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Pereira MG; Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Atella GC; Laboratório de Bioquímica de Lipídios e Lipoproteínas, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: atella@bioqmed.ufrj.br.
المصدر: Acta tropica [Acta Trop] 2024 Sep; Vol. 257, pp. 107281. Date of Electronic Publication: 2024 Jun 08.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6254 (Electronic) Linking ISSN: 0001706X NLM ISO Abbreviation: Acta Trop Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Basel.
مواضيع طبية MeSH: Rhodnius*/parasitology , Rhodnius*/enzymology , Rhodnius*/genetics , Salivary Glands*/parasitology , Salivary Glands*/enzymology , Salivary Glands*/metabolism , Trypanosoma cruzi*/genetics , Trypanosoma cruzi*/enzymology , Phospholipases A2*/metabolism , Phospholipases A2*/genetics, Animals ; Fatty Acids/metabolism ; Chagas Disease/parasitology ; Insect Vectors/parasitology ; Insect Vectors/enzymology
مستخلص: Phospholipases A 2 (PLA 2 ) comprise a superfamily of enzymes that specifically catalyze hydrolysis of the ester bond at the sn-2 position of glycerophospholipids, generating lysophospholipids and fatty acids. In Rhodnius prolixus, one of the main vectors of the Chagas's disease etiologic agent Trypanosoma cruzi, it was previously shown that lysophosphatidylcholine, a bioactive lipid, found in the insect's saliva, contributes to the inhibition of platelet aggregation, and increases the production of nitric oxide, an important vasodilator. Due to its role in potentially generating LPC, here we studied the PLA 2 present in the salivary glands of R. prolixus. PLA 2 activity is approximately 100 times greater in the epithelium than in the contents of salivary glands. Our study reveals the role of the RpPLA 2 XIIA gene in the insect feeding performance and in the fatty acids composition of phospholipids extracted from the salivary glands. Knockdown of RpPLA 2 XIIA significantly altered the relative amounts of palmitic, palmitoleic, oleic and linoleic acids. A short-term decrease in the expression of RpPLA 2 III and RpPLA 2 XIIA in the salivary glands of R. prolixus was evident on the third day after infection by T. cruzi. Taken together, our results contribute to the understanding of the role of PLA 2 in the salivary glands of hematophagous insects and show that the parasite is capable of modulating even tissues that are not colonized by it.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
فهرسة مساهمة: Keywords: Phospholipase A(2); Rhodnius prolixus; Salivary glands; Trypanosoma cruzi
المشرفين على المادة: EC 3.1.1.4 (Phospholipases A2)
0 (Fatty Acids)
تواريخ الأحداث: Date Created: 20240609 Date Completed: 20240729 Latest Revision: 20240729
رمز التحديث: 20240730
DOI: 10.1016/j.actatropica.2024.107281
PMID: 38852917
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-6254
DOI:10.1016/j.actatropica.2024.107281