دورية أكاديمية
Mpox virus (MPXV) vertical transmission and fetal demise in a pregnant rhesus macaque model.
| العنوان: | Mpox virus (MPXV) vertical transmission and fetal demise in a pregnant rhesus macaque model. |
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| المؤلفون: | Krabbe NP, Mitzey AM, Bhattacharya S, Razo ER, Zeng X, Bekiares N, Moy A, Kamholz A, Karl JA, Daggett G, VanSleet G, Morgan T, Capuano SV, Simmons HA, Basu P, Weiler AM, O'Connor DH, Friedrich TC, Golos TG, Mohr EL |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2024 May 30. Date of Electronic Publication: 2024 May 30. |
| نوع المنشور: | Journal Article; Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | Infection with clade I Mpox virus (MPXV) results in adverse pregnancy outcomes, yet the potential for vertical transmission resulting in fetal harm with clade IIb MPXV, the clade that is currently circulating in the Western Hemisphere, remains unknown. We established a rhesus macaque model of vertical MPXV transmission with early gestation inoculation. Three pregnant rhesus macaques were inoculated intradermally with 1.5 × 10^5 plaque forming units (PFU) of clade IIb MPXV near gestational day (GD) 30 and animals were monitored for viremia and maternal and fetal well-being. Animals were euthanized to collect tissues at 5, 14, or 25 days post-inoculation (dpi). Tissues were evaluated for viral DNA (vDNA) loads, infectious virus titers, histopathology, MPXV mRNA and protein localization, as well as MPXV protein co-localization with placental cells including, Hofbauer cells, mesenchymal stromal cells, endothelial cells, and trophoblasts. vDNA was detected in maternal blood and skin lesions by 5 dpi. Lack of fetal heartbeat was observed at 14 or 25 dpi for two dams indicating fetal demise; the third dam developed significant vaginal bleeding at 5 dpi and was deemed an impending miscarriage. vDNA was detected in placental and fetal tissue in both fetal demise cases. MPXV localized to placental villi by ISH and IHC. Clade IIb MPXV infection in pregnant rhesus macaques results in vertical transmission to the fetus and adverse pregnancy outcomes, like clade I MPXV. Further studies are needed to determine whether antiviral therapy with tecovirimat will prevent vertical transmission and improve pregnancy outcomes. One Sentence Summary: Clade IIb Mpox virus infection of pregnant rhesus macaques results in vertical transmission from mother to fetus and adverse pregnancy outcomes. |
| تواريخ الأحداث: | Date Created: 20240610 Latest Revision: 20240610 |
| رمز التحديث: | 20240610 |
| مُعرف محوري في PubMed: | PMC11160791 |
| DOI: | 10.1101/2024.05.29.596240 |
| PMID: | 38853987 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1101/2024.05.29.596240 |
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