دورية أكاديمية
Hybrid Quadrupole Mass Filter - Radial Ejection Linear Ion Trap and Intelligent Data Acquisition Enable Highly Multiplex Targeted Proteomics.
| العنوان: | Hybrid Quadrupole Mass Filter - Radial Ejection Linear Ion Trap and Intelligent Data Acquisition Enable Highly Multiplex Targeted Proteomics. |
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| المؤلفون: | Remes PM; Thermo Fisher Scientific, 355 River Oaks Parkway, San Jose, California 95134., Jacob CC; Thermo Fisher Scientific, 355 River Oaks Parkway, San Jose, California 95134., Heil LR; Thermo Fisher Scientific, 355 River Oaks Parkway, San Jose, California 95134., Shulman N; Department of Genome Sciences, University of Washington, 3720 15th St. NE, Seattle WA 98195., MacLean BX; Department of Genome Sciences, University of Washington, 3720 15th St. NE, Seattle WA 98195., MacCoss MJ; Department of Genome Sciences, University of Washington, 3720 15th St. NE, Seattle WA 98195. |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2024 Jun 01. Date of Electronic Publication: 2024 Jun 01. |
| نوع المنشور: | Journal Article; Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | Targeted mass spectrometry (MS) methods are powerful tools for selective and sensitive analysis of peptides identified by global discovery experiments. Selected reaction monitoring (SRM) is currently the most widely accepted MS method in the clinic, due to its reliability and analytical performance. However, due to limited throughput and the difficulty in setting up and analyzing large scale assays, SRM and parallel reaction monitoring (PRM) are typically used only for very refined assays of on the order of 100 targets or less. Here we introduce a new MS platform with a quadrupole mass filter, collision cell, linear ion trap architecture that has increased acquisition rates compared to the analogous hardware found in the Orbitrap ™ Tribrid ™ series instruments. The platform can target more analytes than existing SRM and PRM instruments - in the range of 5000 to 8000 peptides per hour. This capability for high multiplexing is enabled by acquisition rates of 70-100 Hz for peptide applications, and the incorporation of real-time chromatogram alignment that adjusts for retention time drift and enables narrow time scheduled acquisition windows. Finally, we describe a Skyline external software tool that implements the building of targeted methods based on data independent acquisition chromatogram libraries or unscheduled analysis of heavy labeled standards. We show that the platform delivers ~10x lower LOQs than traditional SRM analysis for a highly multiplex assay and also demonstrate how analytical figures of merit change while varying method duration with a constant number of analytes, or by keeping a constant time duration while varying the number of analytes. Competing Interests: COMPETING FINANCIAL INTERESTS The MacCoss Lab at the University of Washington has a sponsored research agreement with Thermo Fisher Scientific, the manufacturer of the mass spectrometry instrumentation used in this research. Additionally, Michael MacCoss is a paid consultant for Thermo Fisher Scientific. |
| معلومات مُعتمدة: | R24 GM141156 United States GM NIGMS NIH HHS; U01 DK137097 United States DK NIDDK NIH HHS; U19 AG065156 United States AG NIA NIH HHS |
| تواريخ الأحداث: | Date Created: 20240610 Latest Revision: 20240617 |
| رمز التحديث: | 20240617 |
| مُعرف محوري في PubMed: | PMC11160808 |
| DOI: | 10.1101/2024.05.31.596848 |
| PMID: | 38854069 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1101/2024.05.31.596848 |
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