دورية أكاديمية
Plumieride as a novel anti-fungal and anti-inflammatory iridoid against superficial candidiasis in mice.
| العنوان: | Plumieride as a novel anti-fungal and anti-inflammatory iridoid against superficial candidiasis in mice. |
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| المؤلفون: | El-Shiekh RA; Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt. riham.adel@pharma.cu.edu.eg., Meselhy MR; Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt., Elshimy R; Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University, Giza, 12573, Egypt.; Department of Microbiology and Immunology, Egyptian Drug Authority, Cairo, 15301, Egypt., Ibrahim MA; Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt., Ali ME; Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt., Hassanen EI; Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt. |
| المصدر: | BMC complementary medicine and therapies [BMC Complement Med Ther] 2024 Jun 10; Vol. 24 (1), pp. 224. Date of Electronic Publication: 2024 Jun 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101761232 Publication Model: Electronic Cited Medium: Internet ISSN: 2662-7671 (Electronic) Linking ISSN: 26627671 NLM ISO Abbreviation: BMC Complement Med Ther Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : BioMed Central, [2020]- |
| مواضيع طبية MeSH: | Mice, Inbred BALB C* , Candida albicans*/drug effects , Candida albicans*/pathogenicity , Antifungal Agents*/pharmacology , Iridoids*/pharmacology , Anti-Inflammatory Agents*/pharmacology, Animals ; Mice ; Male ; Candidiasis/drug therapy ; Disease Models, Animal |
| مستخلص: | In the past few decades, there has been a notable rise in the occurrence of several types of candidiasis. Candida albicans is the most common cause of superficial fungal infections in humans. In this study, plumieride, one of the major iridoids from Plumeria obtusa L. leaves, was isolated and investigated for its potential against Candida albicans (CA)-induced dermatitis in mice. qRT-PCR was done to assess the impact of plumieride on the expression of the major virulence genes of CA. Five groups (n = 7) of adult male BALB/c mice were categorized into: group I: non-infected mice; group II: mice infected intradermally with 10 7 -10 8 CFU/mL of CA; group III: CA-infected mice treated with standard fluconazole (50 mg/kg bwt.); group IV and V: CA-infected mice treated with plumieride (25- and 50 mg/kg. bwt., respectively). All the treatments were subcutaneously injected once a day for 3 days. Skin samples were collected on the 4th day post-inoculation to perform pathological, microbial, and molecular studies. The results of the in vitro study proved that plumieride has better antifungal activity than fluconazole, manifested by a wider zone of inhibition and a lower MIC. Plumieride also downregulated the expression of CA virulence genes (ALS1, Plb1, and Hyr1). CA-infected mice showed extensive dermatitis, confirmed by strong iNOS, TNF-α, IL-1β, and NF-κB genes or immune expressions. Whereas the treatment of CA-infected mice with plumieride significantly reduced the microscopic skin lesions and modulated the expression of all measured proinflammatory cytokines and inflammatory markers in a dose-dependent manner. Plumieride interfered with the expression of C. albicans virulence factors and modulated the inflammatory response in the skin of mice infected with CA. (© 2024. The Author(s).) |
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| فهرسة مساهمة: | Keywords: Candida albicans; Gene expression; Inflammation; Pathology; Plumieride |
| المشرفين على المادة: | 0 (Antifungal Agents) 0 (Iridoids) 0 (Anti-Inflammatory Agents) |
| تواريخ الأحداث: | Date Created: 20240610 Date Completed: 20240611 Latest Revision: 20240612 |
| رمز التحديث: | 20240612 |
| مُعرف محوري في PubMed: | PMC11163697 |
| DOI: | 10.1186/s12906-024-04508-z |
| PMID: | 38858704 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2662-7671 |
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| DOI: | 10.1186/s12906-024-04508-z |