دورية أكاديمية
Deep analysis of total serum N-glycome suggests glyco-signatures for phospholipase A2 receptor 1-related idiopathic membranous nephropathy diagnosis.
| العنوان: | Deep analysis of total serum N-glycome suggests glyco-signatures for phospholipase A2 receptor 1-related idiopathic membranous nephropathy diagnosis. |
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| المؤلفون: | Cai Y; Shanghai Institute of Precision Medicine, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200125, PR China. Electronic address: caiyan0723@shsmu.edu.cn., Ren W; Department of Nephrology, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, PR China., Li S; Department of Nephrology, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, PR China., Liao R; Shanghai Institute of Precision Medicine, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200125, PR China. Electronic address: rjliao@shsmu.edu.cn., Bian Q; Department of Nephrology, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, PR China. Electronic address: angelbq@126.com. |
| المصدر: | Journal of proteomics [J Proteomics] 2024 Jul 15; Vol. 303, pp. 105223. Date of Electronic Publication: 2024 Jun 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101475056 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-7737 (Electronic) Linking ISSN: 18743919 NLM ISO Abbreviation: J Proteomics Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier |
| مواضيع طبية MeSH: | Glomerulonephritis, Membranous*/blood , Glomerulonephritis, Membranous*/diagnosis , Receptors, Phospholipase A2*/blood , Polysaccharides*/blood , Polysaccharides*/analysis, Humans ; Male ; Female ; Middle Aged ; Biomarkers/blood ; Adult ; Glycomics/methods |
| مستخلص: | Idiopathic membranous nephropathy (IMN) is an antibody-mediated and kidney-specific autoimmune disease, with the antigen phospholipase A2 receptor 1 (PLA2R1) accounting for approximately 70% of IMN cases. Although a variety of new podocyte target antigens and their autoantibodies have been identified, they are still of limited diagnostic and therapeutic value due to lack of high specificity and sensitivity. N-glycans play vital roles in renal system and their pathobiological relevance has become increasingly recognized in many kidney diseases, but not fully explored in IMN. To find possible glyco-signatures for PLA2R1-related IMN diagnosis, we herein established a comprehensive workflow for total serum N-glycome analysis based on our recently developed mass spectrometry (MS)-based N-glycan purification method, named Ultrafast Glycoprotein Immobilization for Glycan extraction (UltraGIG). A total of 191 N-glycans were identified from IMN patients, representing the largest N-glycome dataset in IMN. Compared to healthy controls, up-regulation of sialylation and core-fucosylation as well as down-regulation of galactosylation were observed in PLA2R1-positive IMN patients, and up-regulation of hyper-galactosylation was specific for PLA2R1-negative IMN patients. A six-glycan marker panel consisting of H4N3S1, H4N3F1, H6N4S2, H6H5F1S2, H6N5 and H6N6F1S1, was proposed to aid in the accurate diagnosis of PLA2R1-related IMN, which provided new insights into IMN biomarker study. SIGNIFICANCE: PLA2R1-related IMN is a kidney-specific autoimmune disease with a high risk of developing end-stage renal disease (ESRD) and even kidney failure. Current biomarkers are still of limited diagnostic and therapeutic value due to lack of high specificity and sensitivity. An in-depth MS analysis of total serum N-glycome of PLA2R1-related IMN patients was conducted for the first time. We generated the largest dataset of serum N-glycome for IMN to date, and proposed a novel six-glycan marker panel that may help the accurate diagnosis of PLA2R1-related IMN. Competing Interests: Declaration of competing interest The authors declare no competing financial interest. (Copyright © 2024. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Biomarker; MS-based UltraGIG; PLA2R1-related IMN; Total serum N-glycome |
| المشرفين على المادة: | 0 (Receptors, Phospholipase A2) 0 (PLA2R1 protein, human) 0 (Polysaccharides) 0 (Biomarkers) |
| تواريخ الأحداث: | Date Created: 20240611 Date Completed: 20240627 Latest Revision: 20240718 |
| رمز التحديث: | 20240718 |
| DOI: | 10.1016/j.jprot.2024.105223 |
| PMID: | 38862068 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1876-7737 |
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| DOI: | 10.1016/j.jprot.2024.105223 |