دورية أكاديمية
Flowcytometric data of intermediate-large cell gastrointestinal lymphoma presenting a gross mass in 32 cats - "let them glow in the flow".
| العنوان: | Flowcytometric data of intermediate-large cell gastrointestinal lymphoma presenting a gross mass in 32 cats - "let them glow in the flow". |
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| المؤلفون: | Rütgen BC; Clinical Pathology, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Wolfesberger B; Clinical Unit of Internal Medicine Small Animals, Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Vienna, Austria., Baumgartner D; Clinical Pathology, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Hammer SE; Institute of Immunology, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Groiss S; Institute of Immunology, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Hittmair KM; Diagnostic Imaging, Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Vienna, Austria., Gradner G; Small Animal Hospital Clinic, Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Vienna, Austria., Fuchs-Baumgartinger A; Institute of Pathology, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Donovan TA; The Schwarzman Animal Medical Center, New York, NY, United States., Schwendenwein I; Clinical Pathology, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria. |
| المصدر: | Frontiers in veterinary science [Front Vet Sci] 2024 May 28; Vol. 11, pp. 1378826. Date of Electronic Publication: 2024 May 28 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101666658 Publication Model: eCollection Cited Medium: Print ISSN: 2297-1769 (Print) Linking ISSN: 22971769 NLM ISO Abbreviation: Front Vet Sci Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media S.A., [2014]- |
| مستخلص: | Gastrointestinal lymphoma is the most common form of lymphoma in domestic cats. Aggressive phenotypes are much less common but do bear and unfavorable prognosis. Immunophenotyping by flow cytometry (FCM) is not systematically performed in these patients, because of difficulties in the acquisition of suitable sample material from the gastrointestinal tract. A multimodal diagnostic approach is recommended to improve identification of subtypes targeting patient tailored therapeutic strategies. The aim of this prospective study was to present results of multicolor FCM immunophenotyping in surgically removed gastrointestinal mass and relate them with histopathology using the World Health Organization (WHO) classification and clonality PCR testing. Thirty-two patients were included. Eight cats (25%) had gastric, 23 (72%) had intestinal lymphoma and 1 (3%) had gastric/jejunal lymphoma. Intestinal lymphoma sites were represented by 18 small intestinal, 4 ileocaecal, 1 large intestinal. All gastric lymphomas were diffuse large B-cell lymphoma (DLBCL). Small intestinal lymphomas were 10 enteropathy associated T-cell lymphoma type I (EATL I), 2 enteropathy associated T-cell lymphoma type II (EATL II), 2 peripheral T-cell lymphoma (PTCL), 3 DLBCL and one DLBCL+EATL II. The most common small intestinal FCM T-cell phenotype was CD3 + CD21 - CD4 - CD8 - CD18 + CD5 - CD79 - in 7/10 EATL I and one EATL II. The most frequent FCM B-cell phenotype was CD3 - CD21 + CD4 - CD8 - CD18 + CD5 - CD79 + in 13/17 DLBCL and the DLBCL+EATL II. Clonality PCR results were positive in 87.5% (28/32) of all cases. No cross-lineage rearrangement was observed. IHC and FCM results agreed in 87.5% (28/32) of all cases. When all 3 methods were combined, consistent results were seen in 75% (24/32). This is the first demonstration of a multicolor FCM approach set in context to the gold standard histopathology and clonality testing results. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Rütgen, Wolfesberger, Baumgartner, Hammer, Groiss, Hittmair, Gradner, Fuchs-Baumgartinger, Donovan and Schwendenwein.) |
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| فهرسة مساهمة: | Keywords: WHO classification; feline lymphoma; flow cytometry; gastrointestinal; immunophenotyping |
| تواريخ الأحداث: | Date Created: 20240612 Latest Revision: 20240613 |
| رمز التحديث: | 20240613 |
| مُعرف محوري في PubMed: | PMC11166079 |
| DOI: | 10.3389/fvets.2024.1378826 |
| PMID: | 38863454 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2297-1769 |
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| DOI: | 10.3389/fvets.2024.1378826 |