دورية أكاديمية
Acemannan coated, cobalt-doped biphasic calcium phosphate nanoparticles for immunomodulation regulated bone regeneration.
| العنوان: | Acemannan coated, cobalt-doped biphasic calcium phosphate nanoparticles for immunomodulation regulated bone regeneration. |
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| المؤلفون: | Negi D; Department of Biomedical Engineering, Indian Institute of Technology Ropar, Rupnagar-140 001, Punjab, India. yash@iitrpr.ac.in., Bhavya K; Department of Biomedical Engineering, Indian Institute of Technology Ropar, Rupnagar-140 001, Punjab, India. yash@iitrpr.ac.in., Pal D; Department of Biomedical Engineering, Indian Institute of Technology Ropar, Rupnagar-140 001, Punjab, India. yash@iitrpr.ac.in., Singh Y; Department of Biomedical Engineering, Indian Institute of Technology Ropar, Rupnagar-140 001, Punjab, India. yash@iitrpr.ac.in.; Department of Chemistry, Indian Institute of Technology Ropar, Rupnagar-140 001, Punjab, India. |
| المصدر: | Biomaterials science [Biomater Sci] 2024 Jul 09; Vol. 12 (14), pp. 3672-3685. Date of Electronic Publication: 2024 Jul 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101593571 Publication Model: Electronic Cited Medium: Internet ISSN: 2047-4849 (Electronic) Linking ISSN: 20474830 NLM ISO Abbreviation: Biomater Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, UK : Royal Society of Chemistry |
| مواضيع طبية MeSH: | Bone Regeneration*/drug effects , Cobalt*/chemistry , Cobalt*/pharmacology , Mannans*/chemistry , Mannans*/pharmacology , Nanoparticles*/chemistry , Osteogenesis*/drug effects, Animals ; Mice ; Hydroxyapatites/chemistry ; Hydroxyapatites/pharmacology ; Cell Proliferation/drug effects ; Immunomodulation/drug effects ; Cell Differentiation/drug effects ; Cell Line ; Macrophages/drug effects ; Macrophages/metabolism ; Macrophages/immunology ; Cell Survival/drug effects ; Coated Materials, Biocompatible/chemistry ; Coated Materials, Biocompatible/pharmacology |
| مستخلص: | Biomaterials are used as scaffolds in bone regeneration to facilitate the restoration of bone tissues. The local immune microenvironment affects bone repair but the role of immune response in biomaterial-facilitated osteogenesis has been largely overlooked and it presents a major knowledge gap in the field. Nanomaterials that can modulate M1 to M2 macrophage polarization and, thus, promote bone repair are known. This study investigates a novel approach to accelerate bone healing by using acemannan coated, cobalt-doped biphasic calcium phosphate nanoparticles to promote osteogenesis and modulate macrophage polarization to provide a prohealing microenvironment for bone regeneration. Different concentrations of cobalt were doped in biphasic calcium phosphate nanoparticles, which were further coated with acemannan polymer and characterized. The nanoparticles showed >90% cell viability and enhanced cell proliferation along with osteogenic differentiation as demonstrated by the enhanced alkaline phosphatase activity and osteogenic calcium deposition. The morphology of MC3T3-E1 cells remained unchanged even after treatment with nanoparticles. Acemannan coated nanoparticles were also able to decrease the expression of M1 markers, iNOS, and CD68 and enhance the expression of M2 markers, CD206, CD163, and Arg-1 as indicated by RT-qPCR, flow cytometry, and ICC studies. The findings show that acemannan coated nanoparticles can create a supportive immune milieu by inducing and promoting the release of osteogenic markers, and by causing a reduction in inflammatory markers, thus helping in efficient bone regeneration. As per our knowledge, this is the first study showing the combined effect of acemannan and cobalt for bone regeneration using immunomodulation. The work presents a novel approach for enhancing osteogenesis and macrophage polarization, thus, offering a potent strategy for effective bone regeneration. |
| المشرفين على المادة: | 3G0H8C9362 (Cobalt) 0 (Mannans) 0 (hydroxyapatite-beta tricalcium phosphate) UZ29E6L2X8 (acemannan) 0 (Hydroxyapatites) 0 (Coated Materials, Biocompatible) |
| تواريخ الأحداث: | Date Created: 20240612 Date Completed: 20240709 Latest Revision: 20240709 |
| رمز التحديث: | 20240709 |
| DOI: | 10.1039/d4bm00482e |
| PMID: | 38864476 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2047-4849 |
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| DOI: | 10.1039/d4bm00482e |